439 research outputs found
Tactile Storytelling Using a Transmedia Approach
Get PDFThis project aims to gain an understanding of storytelling using a transmedia approach. Throughout the research, I explored how knowledge is generated using a multi-stage process that moves from poetry writing to tapestry weaving, and then to audio recording. My primary objective has been to use a transmedia approach, considering how different media formats interact, to open up and explore how my personal story is interpreted between myself and the audience. By using a transmedia approach, this research project allows me to create an immersive sensory experience for the audience in a way that provides an example for future design research. By designing, encoding, and studying of artifacts created using the TC-2 Digital Jacquard Loom, this research aims to investigate how woven tapestry can help people visualize and experience emotion. As a result of this research, I have created the media formats of tapestry, poetry and audio using a creative transmedia storytelling process, as well as “Hands-on weaving” workshops to provide opportunities for everyone from non-designers to experienced weavers to conduct personal material exploration. What I have found is that it is possible for emotions to be elicited through weaving by employing a transmedia approach causing a synergistic understanding between the storyteller and audience
Abstract 2190: Theranostical nanoscale metal-organic frameworks-mediated rapid multiplexed microRNA detection and highly therapeutic efficacy in living oral cancer cells
No full textAbstract
Oral cancer represents the sixth most frequent cancer in the world, characterized by high poor prognosis and low survival rate due to local relapse and metastasis. MicroRNAs (miRNAs) have been discovered as diagnostic biomarkers and therapeutic targets for oral cancer. However, one of the major challenges of miRNAs-based theranostical approach is to achieve efficient and safe delivery. Thus, a reliable sensing strategy that can detect miRNAs expression levels in living cancer cells is urgently needed. Herein, a novel sensing platform based on nanoscale metal-organic framework (NMOF) conjugated with the fluorophores-labeled single-stranded DNA probe (p-DNA) was fabricated to monitor and inhibit multiplexed miRNAs expression in living oral cancer cells. The flow cytometry and confocal laser scanning microscopy (CLSM) were carried out to evaluate the expression levels of miRNAs in oral cancer cells. The miRNA inhibition efficiency was examined by RT-PCR. The effects of the complexes on tumor cell invasion and migration were assessed by transwell assay and wound healing. The flow cytometry, colony formation assay and CCK8 were used to assess cell apoptosis and proliferation. The results showed that NMOF works as fluorescence quencher of the labeled p-DNA. In presence of the target miRNA (including miR-7, miR-21, and miR-155), p-DNA was able to rapidly hybridize and release from the NMOF leading to the recovery of fluorescence in living cells, which can be detected in real-time. Moreover, RT-PCR results demonstrated that the multiplexed miRNAs could be effectively downregulated after treatment of p-DNA@NMOF, resulting in the simultaneous suppression of oral cancer cell proliferation, anti-apoptosis, migration and invasion in vitro. In summary, our study suggests that p-DNA@NMOF possess great potential for combining early diagnosis and gene therapy of oral cancer.
Note: This abstract was not presented at the meeting.
Citation Format: Pei Yu, Zehang Zhuang, Guihua Qiu, Haolin Chen, Yuying Zhao, Jinxiang Chen, Xiqiang Liu. Theranostical nanoscale metal-organic frameworks-mediated rapid multiplexed microRNA detection and highly therapeutic efficacy in living oral cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 2190. doi:10.1158/1538-7445.AM2017-2190</jats:p
Revealing the nanodomain structure of silicon oxycarbide via preferential etching and pore analysis
No full textPreferentially etching either carbon or silica from silicon oxycarbide (SiOC) created a porous network as an inverse image of the removed phase. The porous structure was analyzed by gas adsorption, and the experimental results verified the nanodomain structure of SiOC. This work demonstrated a novel approach for analyzing materials containing nanocomposite structures.Guangdong Innovation Team Project [202013N080]; Shenzhen Science and Technology Research Grant [JCYJ20150518092933435, JCYJ20150626110958181]SCI(E)[email protected]; [email protected]
Development of graphene oxide-wrapped gold nanorods as robust nanoplatform for ultrafast near-infrared SERS bioimaging
No full textXuejun Qiu,1 Xinru You,2 Xing Chen,2 Haolin Chen,1 Arvind Dhinakar,3 Songhao Liu,1 Zhouyi Guo,1 Jun Wu,2,4 Zhiming Liu1 1SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology, College of Biophotonics, South China Normal University, 2Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Engineering, Sun Yat-Sen University, Guangzhou, Guangdong, People’s Republic of China; 3Faculty of Engineering, University of Waterloo, Waterloo, ON, Canada; 4Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, Guangzhou, Guangdong, People’s Republic of China Abstract: The rapid development of near-infrared surface-enhanced Raman scattering (NIR SERS) imaging technology has attracted strong interest from scientists and clinicians due to its narrow spectral bandwidth, low background interference, and deep imaging depth. In this report, the graphene oxide (GO)-wrapped gold nanorods (GO@GNRs) were developed as a smart and robust nanoplatform for ultrafast NIR SERS bioimaging. The fabricated GO@GNRs could efficiently load various NIR probes, and the in vitro evaluation indicated that the nanoplatform could exhibit a higher NIR SERS activity in comparison with traditional gold nanostructures. The GOs were prepared by directly pyrolyzing citric acid for greater convenience, and GO@GNRs were fabricated via a facile synthesis strategy. Higher NIR SERS activity, facile synthesis method, excellent biocompatibility, and superb stability make the GO@GNRs/probe complex promising nanoprobes for NIR SERS-based bioimaging applications. Keywords: gold nanorods, graphene oxide, near-infrared bioimaging, surface-enhanced Raman scatterin
Characterizing coastal wind speed gradients using Scanning LiDAR data and mesoscale modeling
No full textThis research attempts to investigate the coastal flow structure horizontally. The flow cases with different flow directions, including perpendicular and parallel to the shoreline directions, are selected by analyzing the New Europe Wind Atlas (NEWA) reanalysis data. After the cases are determined, high-resolution Weather Research and Forecasting Model (WRF) are performed under Yonsei University (YSU) Planetary Boundary Layer (PBL) scheme with ERA5 forcing data. The results are validated against the measurements from long-range wind scanner WindCube 400S. The validated data is analyzed for quantifying the orthogonal wind speed gradient over the study domain. As a result, the WRF, being a mesoscale modelling tool, it captures the orthogonal coastal wind speed gradients reasonably accurate within a 6km range compared with observations. Generally, WRF performs better in the alongshore flow cases, especially in the flow case 2018-10-08 when the stable condition occurs most frequently during the case. The Root Mean Square Error (RMSE) for this case is 0.9m/s. However, the simulation results lost correlations with the observational data in some epochs when the fluctuation of wind speed occurs frequently. To be concrete, during flow case 2018-12-10 when the flow direction is around 330◦ advecting onshore, the WRF simulation can predict the fluctuations correct in magnitude but with shifting in time. For the coastal wind speed gradient, WRF predicts an absolute wind speed difference of 0.5 m/s daily averaged at the typical hub-height over a 1.4km distance for the alongshore flow cases. This difference can enlarge to 1m/s or higher while the low-level jets take place. The long-range wind scanning LiDAR provides a great possibility for observing the flow condition over a wind farm sized domain. Especially for the nearshore wind farm, it compensates for lack of observations on the water.Geoscience and Remote Sensin
Comparison of electroacupuncture in restrained and unrestrained rat models
Get PDFAcupuncture and electroacupuncture (EA) are widely used to treat a variety of diseases including pain. In preclinical research, EA is usually applied by inserting acupuncture needles into the hindlimbs of rats restrained in small tubes or bags. This restrained model of EA not only causes stress-like behaviors but also is limited in stimulating locations and intensities. In 2004, a novel, unrestrained model of EA was introduced. However, these two EA methods have never been directly compared regarding their analgesic effects and other features such as stress. In the present study, we reported similar analgesic effects between restrained and unrestrained EA in rats of acute inflammatory pain induced by intraplantar injection of CFA. In addition, rats receiving unrestrained EA showed less significant stress-like behaviors and tolerated higher current intensity. These advantages suggest that this unrestrained EA method can replace the traditional restrained procedure with similar analgesic effects and allow for more choices of stimulating intensities and locations. © 2013 Haolin Zhang et al.link_to_subscribed_fulltex
Coddingtonia chenyufengi Lin & Li 2024, sp. nov.
No full text<i>Coddingtonia chenyufengi</i> Lin & Li, sp. nov. (Figs 76, 80A) <p>Etymology. The specific name is dedicated to Mr. Yufeng Chen (Beijing, China), who has helped us greatly with this research; noun (name) in genitive case.</p> <p> Diagnosis. The female of the new species is similar to <i>C. erhuan</i> Feng & Lin, 2019 by the copulatory ducts coiling fewer than 4 times around the spermathecae. However, it can be distinguished by the copulatory ducts being wavy (<i>vs.</i> no obvious modifications).</p> <p>Description. Female (holotype). Total length 1.56, carapace 0.55 long, 0.57 wide, opisthosoma 1.06 long, 0.79 wide. Eye sizes and interdistances: AME 0.04, ALE 0.04, PME 0.04, PLE 0.04, AME–AME 0.01, AME–ALE 0.01, PME–PME 0.02, PME–PLE 0.02, AME–PME 0.03, ALE–PLE 0. Chelicerae with 4 promarginal teeth. Leg measurements: I 2.12 (0.66, 0.70, 0.41, 0.35), II 1.86 (0.55, 0.64, 0.36, 0.31), III 1.39 (0.38, 0.46, 0.28, 0.27), IV 1.73 (0.51, 0.63, 0.32, 0.27).</p> <p>Coloration (Fig. 80A). Carapace pear shaped, dark brown, darker medially, edge black, without pattern. Chelicerae, labium dark brown. Sternum tan. Legs dark brown, with dense setae. Opisthosoma conical, dark brown, surface with sparse long setae, venter darker than dorsum.</p> <p>Epigyne (Fig. 76). Epigynal plate nearly rectangular. Spermathecae barely visible through integument. Lateral wings of spermathecae kidney shaped. Copulatory ducts wavy, forming a semicircle ventrolaterally on wings, followed by 3 complete loops around the spermathecae. Spermathecae globose, almost adjacent. Fertilization duct directed at 10:30- o’clock-position from spermatheca.</p> <p>Male. Unknown.</p> <p>Material examined. Holotype ♀ (IZCAS-Ar44904), Vietnam: Hai Phong, Cat Ba National Park, Natural Forest, Sieving, 16.VII.2008, Dinh-Sac Pham leg.</p> <p>Distribution. Known only from the type locality.</p>Published as part of <i>Lin, Yejie, Li, Shuqiang, Mo, Haolin & Wang, Xihao, 2024, Thirty-eight spider species (Arachnida: Araneae) from China, Indonesia, Japan and Vietnam, pp. 4-98 in Zoological Systematics 49 (1)</i> on pages 89-92, DOI: 10.11865/zs.2024101, <a href="http://zenodo.org/record/10941018">http://zenodo.org/record/10941018</a>
Gushangzao pelorosus Lin & Li & Mo & Wang 2024, comb. nov.
No full text<i>Gushangzao pelorosus</i> (Zhu, 1998) comb. nov. (Figs 64A, 65A, 66A, 74A–B) <p> <i>Dipoena pelorosa</i> Zhu, 1998: 243, fig. 160 (♂ ♀).</p> <p> <i>Dipoena pelorosa</i>: Song, Zhu & Chen, 1999: 112, figs 55I, J, O (♂ ♀).</p> <p> <i>Dipoena pelorosa</i>: Yin <i>et al.</i>, 2012: 322, figs 125a–c (♀).</p> <p> Diagnosis. See diagnosis of the species <i>Gushangzao goemon</i> Lin & Li, <b>sp. nov.</b></p> <p>Description. See Zhu (1998).</p> <p>Types. Holotype ♀ and paratypes 5♂ 4♀, China: Shaanxi, Baoji City, Mei Co., Taibai Mt, 12.VII.1991 (MHBU, not examined).</p> <p>Material examined. 4♂ 4♀, China: Sichuan, Guangyuan City, Qingchuan Co., Tangjiahe National Nature Reserve, Motianling (32.6315°N, 104.8403°E, elev. 1807 m), 2.X.2019, Yejie Lin and Tongyao Jiang leg.</p> <p>Distribution. China (Shaanxi, Sichuan, Hunan).</p> <p> Comments. The membranous spermathecae and fertilization ducts attached by a Y-shaped sclerotized structure indicate that this species belongs to <i>Gushangzao</i> Lin & Li, <b>gen. nov.</b> Thus, we transfer it from <i>Dipoena</i> Thorell, 1869 to <i>Gushangzao</i> Lin & Li, <b>gen. nov.</b> and as a new combination.</p> <p> Habitat. Living on the web of <i>Psechrus</i> sp. (Psechridae Simon, 1890)</p>Published as part of <i>Lin, Yejie, Li, Shuqiang, Mo, Haolin & Wang, Xihao, 2024, Thirty-eight spider species (Arachnida: Araneae) from China, Indonesia, Japan and Vietnam, pp. 4-98 in Zoological Systematics 49 (1)</i> on page 75, DOI: 10.11865/zs.2024101, <a href="http://zenodo.org/record/10941018">http://zenodo.org/record/10941018</a>
Mallinella zhoushengboi Lin & Li 2024, sp. nov.
No full text<i>Mallinella zhoushengboi</i> Lin & Li, sp. nov. (Figs 81–83) <p>Etymology. The specific name is dedicated to Mr. Shengbo Zhou (Shenyang, China), who has helped us greatly with this research; noun (name) in genitive case.</p> <p> Diagnosis. <i>Mallinella zhoushengboi</i> Lin & Li, <b>sp. nov.</b> can be distinguished from all other Chinese species by the curved, hook-shaped tip of the tegular apophysis and the epigynal atrium posterior edge with a scape.</p> <p>Description. Male (holotype). Total length 5.54, carapace 2.47 long, 1.96 wide, opisthosoma 2.98 long, 2.11 wide. Eye sizes and interdistances: AME 0.20, ALE 0.17, PME 0.16, PLE 0.17, AME–AME 0.05, AME–ALE 0.08, PME–PME 0.10, PME–PLE 0.21, AME–PME 0.12, ALE–PLE 0.05. Leg measurements: I 7.38 (1.97, 2.22, 1.80, 1.39), II 7.07 (1.84, 2.14, 1.76, 1.33), III 6.64 (1.46, 1.91, 2.04, 1.23), IV 9.19 (2.05, 2.61, 2.87, 1.66).</p> <p>Coloration (Fig. 83A). Entire prosoma, including legs, reddish brown. Dorsum of opisthosoma sepia. Opisthosoma oval, dark brown, dorsum with 3 pairs of pale oblong spots, anterior one largest, followed by hourglass-shaped spots. Spinnerets yellow-brown.</p> <p>Palp (Fig. 81). Femur three times longer than wide. Retrolateral tibial apophysis digitiform, tip blunt. Tegular apophysis rostrated; mesal ridge tip blunt; baso-prolateral tooth triangular; with broad base. Embolus relatively broad, bifurcated subterminally, mesal ramus longer than lateral ramus.</p> <p>Female (one paratype Ar44923). Total length 6.80, carapace 3.55 long, 2.48 wide, opisthosoma 3.56 long, 2.60 wide. Eye sizes and interdistances: AME 0.18, ALE 0.20, PME 0.19, PLE 0.22, AME–AME 0.07, AME–ALE 0.09, PME–PME 0.08, PME–PLE 0.24, AME–PME 0.12, ALE–PLE 0.03. Leg measurements: I 6.48 (1.87, 2.04, 1.33, 1.24), II 5.98 (1.63, 1.87, 1.28, 1.20), III 6.11 (1.45, 1.85, 1.65, 1.16), IV 7.75 (1.74, 2.29, 2.33, 1.39).</p> <p>Coloration (Fig. 83). Similar to that of male except 4 pairs of spots and white spots posteriorly, without scutum.</p> <p>Epigyne (Fig. 81). Atrium broad, anterior edge slightly curved and posterior edge with scape. Lateral border of epigynal plate tip blunt. Spermathecae enlarged, rounded apically, separated by about their diameter</p> <p>Material examined. Holotype ♂ (IZCAS-Ar44922), China: Chongqing, Chengkou Co., 20.VIII.2023, Yuyang Zhou leg. Paratype 1♀ (IZCAS-Ar44923), same data as holotype.</p> <p>Distribution. Known only from the type locality.</p> <p> <b>Funding</b> This research was funded by the Science & Technology Fundamental Resources Investigation Program of China (2023FY100200).</p> <p> <b>Acknowledgements</b> The manuscript benifitted greatly from comments by Yuri M. Marusik (Magadan, Russia), Alireza Zamani (Turku, Finland) and Quanyu Ji (Hebei, China). Theo Blick (WSC) checked etymology. Sarah Crews checked the English of the final version. Can Zhang (Yunnan), Danyang Zhou (Zhejiang), Fan Gao (Jiangsu), Fengyuan Li (Beijing), Guo Zheng (Liaoning), Haolin Mo (Guangxi), Huihang Cai (Zhejiang), Jiang Chang (Zhejiang), Jie Liu (Hubei), Jincheng Liu (Beijing), Linghui Lin, Qi Cao, Qianle Lu (Guangdong), Shanmi Zheng (Sichuan), Tongyao Jiang (Beijing), Xiaoxiao Wang, Xin Xie (Guangdong), Yafei Wang, Yanfeng Tong (Liaoning), Yiduo Wang (Guangdong), Yucheng Lin (Sichuan), Yuyang Zhou (Chongqing), Zhe Zhao (Beijing), Zhigang Chen (Yunnan), Zhiyuan Yao (Liaoning) and Zixian Fang (Zhejiang) from China and Dinh-Sac Pham (Hanoi, Vietnam) helped with field work. Lan Yang (Beijing) helped with measurements. Danyang Zhou (Zhejiang, China), Fan Gao (Jiangsu), Haolin Mo (Guangxi), Jiang Chang (Zhejiang), Li He (Sichuan), Qianle Lu (Guangdong), Xin Xie (Guangdong), Yanbin Yao (Fujian) and Zehao Ma (Yunnan) from China took the photographs.</p>Published as part of <i>Lin, Yejie, Li, Shuqiang, Mo, Haolin & Wang, Xihao, 2024, Thirty-eight spider species (Arachnida: Araneae) from China, Indonesia, Japan and Vietnam, pp. 4-98 in Zoological Systematics 49 (1)</i> on pages 95-96, DOI: 10.11865/zs.2024101, <a href="http://zenodo.org/record/10941018">http://zenodo.org/record/10941018</a>
Face recognition using color information of image
No full textFace recognition is a computer application that able to identify the human face. Computer will study and transformer each of individual face into certain computer format and store in data base with individual labelling. New face will compare to face data base, finishing recognition and generate result. Nowadays, face recognition technology is widely using in our daily life especially in security systems.Bachelor of Engineerin
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