10,674 research outputs found

    Analysis by high-resolution two-dimensional electrophoresis of differentiation-dependent alterations in cytosolic protein pattern of HL-60 leukemic cells

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    HL-60 leukemic cells were differentiated along the neutrophilic pathway with retinoic acid (RA) or along the monocytic pathway with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). Using a high-resolution two-dimensional electrophoresis technique and subsequent silver staining, differentiation-dependent changes in cytosolic protein pattern of HL-60 cells were analysed and were compared with the cytosolic protein pattern of human neutrophils. The amount of 64 and 50 out of a total of 632 proteins studied was increased or decreased in RA- and 1,25(OH)2D3-differentiated HL-60 cells, respectively, in comparison to undifferentiated HL-60 cells. Thirty-three of these proteins were similarly altered in RA- and 1,25(OH)2D3-differentiated HL-60 cells. Twenty-two and 25 of the proteins altered in amount in RA- or 1,25(OH)2D3-differentiated HL-60 cells versus undifferentiated HL-60 cells were similarly altered in human neutrophils in comparison to undifferentiated HL-60 cells. Seven and 10 of the proteins altered in amount in RA- or 1,25(OH)2D3-differentiated HL-60 cells had specific equivalents in neutrophil cytosol. Our results show (i) that neutrophilic and monocytic differentiation is associated with decreases and increases in amount of cytosolic proteins; (ii) that both differentiation processes share a common set of alterations; and (iii) are associated with specific alterations in protein amount

    Stegana (Steganina) oligochaeta Chen & Chen, 2012, sp. nov.

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    Stegana (Steganina) oligochaeta sp. nov. (Figs 28–34) Diagnosis. This species is related to S. protuberans sp. nov., but clearly distinguishable from the latter by the epandrium and aedeagus (in S. protuberans: epandrium not broadened on posterior margins, approximately twice as high as wide; aedeagus lacking sclerotized, round, minute processes basoventrally). Description. Male terminalia: Epandrium broadened on posterior margins, approximately 3 / 5 as high as wide, protruded ventrally (Fig. 28). Cercus with sporadic pubescence (Fig. 28). Surstylus with 8 prensisetae submedially (Fig. 29). Tenth sternite sclerotized and deeply separated basally, protruded apically (Fig. 30). Paramere with ca. 2 sensilla (Figs 31, 32). Aedeagus with scalelike, minute processes dorsally and several sclerotized, round, minute processes basoventrally, slightly semilunar on opening in ventral view (Figs 31, 32). Dorsomedial projection of gonopods with serrated, minute processes distally (Fig. 34). Measurements. BL = 2.78 mm in holotype (13 paratype 2.89 mm), ThL = 1.40 mm (1.36 mm), WL = 2.94 mm (3.09 mm), WW = 1.26 mm (1.38 mm), arb = 9 / 6 (9 / 5), avd = 0.80 (0.91), adf = 1.43 (1.38), flw = 1.86 (1.63), FW/HW = 0.48 (0.49), ch/o = 0.21 (0.24), prorb = damaged (damaged), rcorb = 0.85 (damaged), vb = 0.33 (0.40), dcl = 0.48 (0.48), presctl = 0.44 (0.45), sctl = damaged (damaged), sterno = 0.88 (0.86), orbito = 2.00 (2.25), dcp = 0.19 (0.17), sctlp = 1.78 (1.90), C = 1.97 (2.00), 4 c = 1.17 (1.06), 4 v = 1.90 (1.76), 5 x = 1.45 (1.33), ac = 11.67 (12.00), M = 0.53 (0.47), C 3 F = 0.68 (0.66). Type material. Holotype 3 (SCAU, No. 120768), CHINA: Mt. Jizu, Binchuan, Yunnan, 1900 m, 23.vii. 2006, HL Cao. Paratype: CHINA: 13 (SCAU, No. 120769), Mt. Wuliang, Nanjian, Yunnan, 25.vii. 2006, HL Cao. Distribution. China (Yunnan). Etymology. A combination of the Greek words: oligos (= few) and chaite (= bristle, seta), referring to the cercus with sporadic pubescence.Published as part of Chen, Xi-Peng & Chen, Hong-Wei, 2012, Ten new species of the Stegana (Steganina) shirozui species group (Diptera, Drosophilidae) from China, pp. 24-37 in Zootaxa 3333 on pages 29-30, DOI: 10.5281/zenodo.21543

    Stegana (Steganina) montana Chen & Chen, 2012, sp. nov.

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    Stegana (Steganina) montana sp. nov. (Figs 21–27) Diagnosis. This species is related to S. dianensis sp. nov., but clearly distinguishable from the latter by the scutellum and tenth sternite, in this species: scutellum yellow medially, black laterally; tenth sternite expanded distally (Fig. 23). Description. Male terminalia: Epandrium broadened on posterior margins, wider than 3 / 5 high, pointedly protruded ventrally (Fig. 21). Cercus lacking pubescence (Fig. 21). Surstylus with 6 prensisetae submedially (Fig. 22). Tenth sternite furcated basally (Fig. 23). Paramere with ca. 3 sensilla (Figs 24, 25). Aedeagal opening slightly infundibular in ventral view (Figs 24, 25). Gonopods acute apically, with broadened, medially depressed projection dorsomedially (Fig. 27). Measurements. BL = 3.04 mm in holotype (range in 43 and 5 Ƥ paratypes: 2.80–3.20 mm in 3, 2.90–3.25 mm in Ƥ), ThL = 1.85 mm (1.83–1.88 mm in 3, 1.60–1.80 mm in Ƥ), WL = 3.35 mm (3.20–3.50 mm in 3, 3.28– 3.65 mm in Ƥ), WW = 1.50 mm (1.45–1.50 mm in 3, 1.44–1.45 mm in Ƥ), arb = 9 / 5 (7–9 / 4–5), avd = 0.75 (0.68– 0.83), adf = 1.78 (1.45–1.64), flw = 1.89 (1.71–1.83), FW/HW = 0.41 (0.40–0.43), ch/o = 0.16 (0.15–0.16), prorb = 1.25 (1.18–1.19), rcorb = 0.75 (0.75–0.88), vb = 0.47 (0.33–0.47), dcl = 0.46 (0.41–0.52), sctl = 1.35 (1.30–1.45), sterno = 0.83 (0.82–0.86), orbito = 1.60 (1.60–1.80), dcp = 0.23 (0.22–0.23), sctlp = 2.11 (1.78 –2.00), C = 2.00 (1.77–2.09), 4 c = 1.03 (1.06–1.10), 4 v = 1.60 (1.50–1.67), 5 x = 1.60 (1.45–1.67), ac = 12.00 (10.60 –12.00), M = 0.46 (0.43–0.52), C 3 F = 0.70 (0.71–0.78). Type material. Holotype 3 (SCAU, No. 120745), CHINA: Mt. Wuliang, Nanjian, Yunnan, 2450 m, 25.vii. 2006, HL Cao. Paratypes: CHINA: 1238 Ƥ (333 Ƥ in KIZ; 935 Ƥ in SCAU, Nos 120746 - 59), same data as holotype except 25–28.vii. 2006, HL Cao, HZ Cao, T Li; 632 Ƥ (SCAU, Nos 120760 - 67), Mt. Wuliang, Jingdong, Yunnan, 4.viii. 2006, HL Cao, HZ Cao, T Li; 532 Ƥ (SCAU, No. 121081 - 87), Menghai, Xishuangbanna, Yunnan, 21 ° 50 ' N, 100 °08' E, 1940 m, 8.iv. 2011, JM Lu, ZF Shao, YR Su, SJ Yan. Distribution. China (Yunnan). Etymology. From the Latin word “montanus” (= montaneous), referring to the specimens collected in mountains.Published as part of Chen, Xi-Peng & Chen, Hong-Wei, 2012, Ten new species of the Stegana (Steganina) shirozui species group (Diptera, Drosophilidae) from China, pp. 24-37 in Zootaxa 3333 on pages 28-29, DOI: 10.5281/zenodo.21543

    Experimental Check of Deceleration of Neutral Beam-Injected Energetic Ions in the HL-2A Tokamak

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    Short pulses of a deuterium neutral beam (NB) with a duration of less than ? 5 ms were co-injected into magnetohydrodynamic (MHD)-quiescent ohmic deuterium plasmas of the HL-2A tokamak to study the variation of the slowing-down time in two different electron temperature environments. Analyses were made for the decay rate of D-D neutrons produced by beam-plasma interaction following NB turn-off, i.e., experimentally observed neutron decay rates were compared with those predicted by a classical slowing-down model. The results suggest the beam ions decelerate without significant loss in the HL-2A tokamak in round terms. When the critical energy for beam ions is higher than the beam injection energy, it seems that a small fraction of the beam ions is lost.journal articl

    Impact of the Interlayer Distance between Graphene and MoS2 on Raman Enhancement

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    Scattering; substrate; functionalization; nanoparticles; spectroscopy; sensitivity; monolayer; platfor

    Stegana (Steganina) protuberans Chen & Chen, 2012, sp. nov.

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    Stegana (Steganina) protuberans sp. nov. (Figs 51–57) Description. Male terminalia: Epandrium strongly protruded ventrally, not broadened on posterior margins, approximately twice as high as wide (Fig. 51). Cercus with sparse pubescence (Fig. 51). Surstylus with 8 prensisetae submedially (Fig. 52). Tenth sternite sclerotized and deeply separated basally, protruded apically (Fig. 53). Paramere with ca. 3 sensilla (Figs 54, 55). Aedeagus with scalelike, minute processes dorsally, slightly semilunar on opening in ventral view (Figs 54, 55). Dorsomedial projection of gonopods with serrated, minute processes distally (Fig. 57). Measurements. BL = 2.75 mm in holotype (range in 3 Ƥ paratypes: 2.22–2.80 mm), ThL = 1.40 mm (1.70– 1.84 mm), WL = 2.84 mm (3.55–3.95 mm), WW = 1.24 mm (1.48–1.54 mm), arb = 7 / 5 (7 / 5–9 / 5), avd = 0.82 (0.75–0.87), adf = 1.57 (1.50 – 1.50), flw = 1.71 (1.63–1.75), FW/HW = 0.48 (0.46–0.48), ch/o = 0.27 (0.21–0.28), prorb = 1.25 (1.25–1.26), rcorb = 0.75 (0.67–0.73), vb = 0.36 (0.40–0.47), dcl = 0.47 (0.46–0.49), presctl = damaged (0.54–0.59), sctl = 1.39 (1.40), sterno = 0.80 (0.83 –1.00), orbito = 2.00 (1.80 –2.00), dcp = 0.21 (0.18– 0.22), sctlp = 1.70 (1.69 –2.00), C = 2.00 (1.98–2.23), 4 c = 1.17 (1.00– 1.07), 4 v = 1.57 (1.70–1.80), 5 x = 1.45 (1.20–1.38), ac = 11.67 (11.00–13.00), M = 0.53 (0.45–0.47), C 3 F = 0.57 (0.64–0.69). Type material. Holotype 3 (SCAU, No. 120778, CHINA: Mt. Wulian, Nanjian, Yunnan, 2200 m, 25– 27.vii. 2006, HL Cao. Paratypes: CHINA: 3 Ƥ (SCAU, Nos 120779 - 81), same data as holotype. Distribution. China (Yunnan). Etymology. From the Latin word: protuberans (= protruding), referring to the gonopods with 1 projection dorsomedially.Published as part of Chen, Xi-Peng & Chen, Hong-Wei, 2012, Ten new species of the Stegana (Steganina) shirozui species group (Diptera, Drosophilidae) from China, pp. 24-37 in Zootaxa 3333 on pages 32-33, DOI: 10.5281/zenodo.21543

    Patient characteristics at the time of HL diagnosis among HL-NSCLC and HL-SCLC.

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    Patient characteristics at the time of HL diagnosis among HL-NSCLC and HL-SCLC.</p
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