723 research outputs found
The Escherichia coli motA flagellar gene as a potential integration site for large synthetic DNA
No full textEscherichia coli is used as a chassis for many synthetic biology applications. However, the limitations of maintaining recombinant plasmids extra-chromosomally include increased metabolic burden to the host, constant selective pressure, variable plasmid copy number and plasmid instability that leads to curing. Hence, to overcome these limitations, DNA constructs are integrated into the bacterial chromosome to allow stable control of copy number and to reduce the metabolic burden towards the surrogate host. Non-essential E. coli flagellar genes have been proposed as potential chromosomal insertion target sites. In this study, we validated and compared the efficiency of two loci, namely motA and flgG, as target sites for synthetic biology applications. To enable this comparison, a dual reporter strain (DRS) that utilises two reporter proteins, EforRED and Venus, was developed as a test case. Initially, a yellow reporter plasmid k14.1_Venus was constructed and subsequently used as the plasmid backbone for the generation of two other plasmids, k14.1_eforRED and pcat_Venus, required to build the dual reporter strain. In the DRS, the eforRED gene was inserted into flgG whereas motA was disrupted by Venus. This mutant strain was defective in motility (p<0.001) but growth rate was unaffected. The fluorescence emitted by Venus was higher (p<0.05) compared to EforRED, suggesting that motA is the better chromosomal target locus compared to flgG. Hence, this study proposes the use of E. coli motA as the site for chromosomal insertion for future synthetic biology applications
Consumer behaviour of mobile phone users : a demographic and psychographic study / Yip Phoi Chee.
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Shear strength and deformability of rock joints
No full textThesis (M.S.)--Massachusetts Institute of Technology, Dept. of Civil Engineering, 1979.MICROFICHE COPY AVAILABLE IN ARCHIVES AND ENGINEERING.Bibliography: leaves 174-178.by Chee-Kuen Yip.M.S
sj-docx-1-aph-10.1177_10105395231211978 – Supplemental material for Breastfeeding Prevalence and Factors Affecting Breastfeeding Duration Among Urban Multi-ethnic Malaysian Mothers: A Single-Center University Hospital Study
No full textSupplemental material, sj-docx-1-aph-10.1177_10105395231211978 for Breastfeeding Prevalence and Factors Affecting Breastfeeding Duration Among Urban Multi-ethnic Malaysian Mothers: A Single-Center University Hospital Study by Kai Voon Heng, Lucy Chai See Lum, Emer Breen, Chuan Way Lye, Wai Chee Kuan, Saw Huan Goh, Ling Hang Tan and Kar Mun Yip in Asia Pacific Journal of Public Health</p
University of California Press eScholarship editions in process
No full textIn this collection of passionately argued essays, the internationally acclaimed poet and critic Wai-lim Yip calls Western scholarship to account for its treacherous representation of non-Western literature. Yip moves from Plato to Hans-Georg Gadamer, from Chuang-tzu to Mao Tse-tung, from John Donne to Robert Creeley, as he attempts to create a double consciousness that includes the state of mind of the original author and the expressive potentials of the target language. He aims, first, to expose the types of distortions that have occurred in the process of translation from one language to another and, second, to propose guidelines that will prevent this kind of linguistic violence in the future
Chi-Square and F Approximations of Hetelling's Generalized T0^2
No full textTitle: Chi-Square and F Approximations of Hetelling's Generalized T0^2, Author: Davin Y.N. Yip. Location: ThodeChi-square approximation of Retelling's generalized T0^2 have
been investigated by Professor Tiku. In this project, further results
on the approximation are presented and examined. Also, an F approximation
for the distribution of Retelling's generalized T0^2 is proposed. The
results are compared with other approximations and discussed.ThesisMaster of Science (MSc
Common polymorphisms in TLR4 gene associated with susceptibility to pulmonary tuberculosis in the sudanese
No full textAuthor name used in this publication: Leung, K.H.Author name used in this publication: Yip, S.P.2012-2013 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishedGreen (AAM
Development of a codon optimization strategy using the efor RED reporter gene as a test case
No full textFei xian xing jie zhi he wai jia jing tai ci chang dui biao mian deng li zi se san guan xi de ying xiang
No full textLi, Ming Yip = 非線性介質和外加靜態磁場對表面等離子色散關係的影響 / 李銘業.Thesis M.Phil. Chinese University of Hong Kong 2014.Includes bibliographical references (leaves 88-91).Abstracts also in Chinese.Title from PDF title page (viewed on 01, November, 2016).Li, Ming Yip = Fei xian xing jie zhi he wai jia jing tai ci chang dui biao mian deng li zi se san guan xi de ying xiang / Li Mingye
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