13,299 research outputs found
ACTIVITY OF CHLORAMPHENICOL ACETYLTRANSFERASE OVERPRODUCED IN ESCHERICHIA-COLI WITH WILD-TYPE AND MUTANT GROEL
No full textThe homo-oligomeric protein chloramphenicol acetyltransferase (CAT) has previously been shown to interact with a chaperone GroEL in vitro, suggesting a possible involvement of GroEL in CAT assembly. CAT was overproduced to various levels in the presence and absence of GroEL overproduction, and in groEL mutants. CAT was accumulated to 9-45% of total cellular protein in a fully soluble form, without formation of inclusion bodies. In all cases even with groEL mutants, CAT specific activity was shown proportional to the amount of protein produced, indicating the formation of active trimer CAT structure does not need GroEL in Escherichia coli
Strong suppression of chloramphenicol acetyltransferase expression by inducing T7 RNA polymerase
No full textSuppression of gene expression can be used as a way to study gene regulation. Expression of chloramphenicol acetyltransferase (CAT) under E. coli promoter was inhibited by 92 % by induction of T7 RNA polymerase which transcribes from a T7 promoter opposing E. coli promoter
Disease candidate gene approach using both expression quantitative trait locus and biological pathway analices
No full textEffects of Mutations in TATA Element of Phage T7 and SP6 Promoters on Transcription Initiation Efficiency
No full text
In Vitro Transcription Termination Efficiencies of the T7 Terminator T* for T7 and SP6 RNA Polymerases
No full text- …
