68,514 research outputs found

    Measuring and analyzing German and Spanish customer satisfaction of using the iPhone 4S Mobile Cloud service

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    This paper presents the customer satisfaction analysis for measuring popularity in the Mobile Cloud, which is an emerging area in the Cloud and Big Data Computing. Organizational Sustainability Modeling (OSM) is the proposed method used in this research. The twelve-month of German and Spanish consumer data are used for the analysis to investigate the return and risk status associated with the ratings of customer satisfaction in the iPhone 4S Mobile Cloud services. Results show that there is a decline in the satisfaction ratings in Germany and Spain due to economic downturn and competitions in the market, which support our hypothesis. Key outputs have been explained and they confirm that all analysis and interpretations fulfill the criteria for OSM. The use of statistical and visualization method proposed by OSM can expose unexploited data and allows the stakeholders to understand the status of return and risk of their Cloud strategies easier than the use of other data analysis

    Diversity-Oriented Fluorescence Library Approach (DOFLA) for Discovery of Cell-Permeable Probes for Applications in Live Cell Imaging

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    Fluorescent small-molecule probes can enter living cells and interact with molecules of interest to monitor specific organelle functions. The ideal imaging probes should be able to penetrate cellular membranes and should exhibit minimal background fluorescence signal in order to allow monitoring specific cellular functions without disrupting cell physiology or viability. In this chapter, we will introduce a powerful strategy, namely the diversity-oriented fluorescence library approach (DOFLA), which involves synthesizing a large number of fluorescent molecules using combinatorial chemistry. We present the concepts of DOFLA, DOFL construction techniques, and focus on the applications of cell-based DOFLA screening in probe discovery and subcellular behaviors analysis of small-molecule probes in living cells. This chapter highlights the advantages of DOFLA and provides a series of case studies of discovery of cell-permeable specific probes. ? 2021, Springer Science+Business Media, LLC, part of Springer Nature.11Nscopu
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