8,600 research outputs found
Kinetic Study of Organic Acid Formations and Growth of Anaerobiospirillum succiniciproducens During Continuous Cultures
No full textSuccinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically grown in glucose-fed continuous cultures using glucose as a carbon source, and the metabolic flexibility of A. succiniciproducens in response to varying glucose concentrations and dilution rates was examined. Both succinic acid (SA) and acetic acid (AA) formation was growth-associated, and their growth-rate-related coefficients (K(SA/X), K(AA/X)) and nongrowth-rate-related coefficients (K'(SA/X), K'(AA/X)) were slightly influenced by glucose concentrations. A high glucose concentration (38 g/l) and high growth rate (0.63 h(-1)) did not induce by-product formation.This work was supported by a grant from Research
Promotion Program funded by Gyeonggi Province and by
Priority Research Centers Program through the National
Research Foundation of Korea (NRF) funded by the Ministry
of Education, Science and Technology (20090093826) (PC
Lee) and by the Genome based Integrated Bioprocess
Development Project from the Ministry of Education,
Science and Technology (SY Lee and HN Chang)
Cell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens using an internal membrane filtration system
No full textCell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically carried out using an internal membrane filter module in order to examine the physiological response of A. succiniciproducens to a high-cell-density environment. The optimal growth of A. succiniciproducens and its enhanced succinic acid productivity were observed under CO2-rich conditions, established by adding NaHCO3 and Na2CO3, in the cell recycled system. A. succiniciproducens grew up to 6.50 g-DCW/l, the highest cell concentration obtained so far, in cell recycled cultures. The cells did not change their morphology, which is known to be easily changed in unfavorable or stress environments. The maximum productivity of succinic acid was about 3.3 g/l/h, which is 3-3 times higher than those obtained in batch cultures. These results can serve as a guide for designing highly efficient cell recycled systems for succinic acid at a commercial level.This work was supported by the Genome-based Integrated
Bioprocess Development Project of the Ministry of Science
and Technology through the Korea Science and Engineering
Foundation. Further support by the LG Chem Chair
Professorship (SYL) is appreciated
Succinic Acid Production by Anaerobiospirillum succiniciproducens ATCC 29305 Growing on Galactose, Galactose/Glucose, and Galactose/Lactose
No full textSuccinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically grown on galactose, galactose/glucose, or galactose/lactose in order to study its galactose fermentation. Unlike a previous report, A. succiniciproducens was found to efficiently metabolize galactose as the sole carbon source at a rate of 2.4 g/g-DCW/h and produced succinic acid with as high a yield of 87% as with using glucose. When glucose and galactose were present, A. succiniciproducens metabolized both sugars simultaneously. Furthermore, when lactose and galactose coexisted, lactose did not inhibit the galactose fermentation of A. succiniciproducens. Therefore, co-utilization of galactose and other sugars can improve the productivity and economy of bio-based succinic acid processes.This work was supported by grants from Ajou University
to PCL and by the Genome-based Integrated Bioprocess
Development Project of the Ministry of Science and
Technology through the Korea Science and Engineering
Foundation. Further support by the LG Chem Chair
Professorship (SYL) is appreciated
A PC parallel port button box provides millisecond response time accuracy under Linux
Full text linkFor psychologists, it is sometimes necessary to measure people's reaction times to the nearest millisecond. This article describes how to use the PC parallel port to receive signals from a button box to achieve millisecond response time accuracy. The workings of the parallel port, the corresponding port addresses, and a simple Linux program for controlling the port are described. A test of the speed and reliability of button box signal detection is reported. If the reader is moderately familiar with Linux, this article should provide sufficient instruction for him or her to build and test his or her own parallel port button box. This article also describes how the parallel port could be used to control an external apparatus
Occupational health services in medium- and large-sized factories and industrial zones of Taiwan
No full text
Genetic variation of viral protein 1 gene of field strains of waterfowl parvoviruses and their attenuated derivatives
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