40,929 research outputs found
Association of Proline-Directed Protein Kinase F-a with Tumorigenesis, Invasion, and Poor Prognosis of Human Colon Carcinoma
No full textBACKGROUND. Initial studies revealed that the multisubstrate proline- directed protein kinase F-A (PDPK F-A) is overexpressed in various types of human carcinomas relative to normal controls. Suppression of overexpressed PDPK F-A inhibits the growth of cancer cells, suggesting a role of this PDPK in human malignancy. In this study, we combine immunohistologic, molecular, cellular, animal, and clinicopathologic studies to demonstrate an essential and critical role of PDPK F-A in progression and poor prognosis of human colon carcinoma. METHODS. The stable antisense clones of human colon carcinoma cells with specific suppression of PDPK F-A were established for tumorigenesis and invasion studies. In immunohistologic and clinicopathologic studies, the expression and localization of PDPK F-A were analyzed by immunohistochemical staining of the specimens obtained from human colon carcinoma patients with Dukes Stage B/C. RESULTS. Initial molecular and cellular studies revealed that the antisense clone of colon carcinoma cells (COLO-205) with specific suppression of PDPK F-A dramatically lost capabilities of adhesion, chemotaxis, and invasion when compared with the parental or control- transfected colon carcinoma cells. This is the first indication of an association of overexpressed PDPK F-A with colon carcinoma progression. In agreement with this notion, the in vivo study also revealed that the mice injected with the antisense clone with low-level PDPK F-A only developed very small tumors ( 5 cm(3)) under identical conditions. Pathologic evaluation revealed invasion to the muscle layer in all tumors formed by the parental cells. In contrast, there was no sign of invasion in mice injected with the antisense clone, confirming an essential role of PDPK F-A in colon carcinoma progression. Clinicopathologic study also revealed that PDPK F-A is preferentially overexpressed in the invasive area of colon carcinomatous tissues and overexpression of PDPK F-A is statistically and closely correlated with venous/lymphatic infiltration, lymph node metastasis, and poor prognosis of colon carcinoma patients with Dukes Stage B/C. CONCLUSIONS. The results demonstrate an essential and critical role of overexpressed PDPK F-A in progression and poor prognosis of colon carcinoma patients. Suppression of overexpressed PDPK F-A may provide a new powerful adjuvant approach to prevent human colon carcinoma progression and poor prognosis after surgery and chemotherapy
Proliferative Activity, Apoptosis and Expression of Oestrogen Receptor and Bcl-2 Oncoprotein in Canine Mammary Gland Tumours
No full textSamples of 39 canine mammary gland tumours (MGTs) were examined immunohistochemically for oestrogen receptor (ER- alpha), Bcl-2 protein and Ki67 antigen, and by TUNEL assay for apoptosis. ER-alpha was expressed by 80% (31/39) of the tumours, including all of the 15 benign tumours and 67% (16/ 42) of the malignant tumours. ER-alpha expression was greater in the benign than in the malignant tumours (P < 0. 01). Bcl-2 protein was detected in 62% (24/39) of the MGTs, of which 67% (10/15) were benign and 58% (14/24) malignant. No significant difference in Bcl-2 expression between benign and malignant tumours was detected. The Ki67 and TUNEL indices were greater in malignant than in benign tumours (P < 0.01). Correlation analysis suggested that ER-a and Bcl-2 expression were related , but this observation lacked statistical significance. The levels of cell proliferation and apoptosis did not appear to be significantly correlated with the expression of Bcl-2. A positive relationship was apparent between cell proliferation and apoptosis, whilst a negative correlation between ER-a and cell proliferation was demonstrated. In conclusion, the suggestion of a positive correlation between ER-alpha and Bcl-2 in canine MGTs indicates that ER may be the regulator of Bcl-2 protein, as in human breast cancer. In contrast to cell proliferation and apoptosis, ER-a and Bcl-2 expression were greater in benign MGTs than in their malignant counterparts. (c) 2005 Elsevier Ltd. All rights reserved
Calcium Concentration in Rat Liver Mitochondria during Anoxic Incubation
No full textBackground and Purpose: The impairment of mitochondrial respiratory function is an important problem in anoxia- reoxygenation injury in animal cells. This study investigated the role of mitochondrial calcium ions (Ca 2+) in a model of anoxic incubation in isolated rat liver mitochondria. Methods: Mitochondria isolated from Wistar rat liver were subjected to anoxic incubation for 5 to 60 minutes. Changes in oxygen consumption by anoxic mitochondria were recorded using polarography. Changes in NAD(P)H fluorescence were recorded simultaneously. Intramitochondrial matrix free calcium concentration ([Ca2+] ) was measured using the fluo-3/AM loading method. Respiratory function in anoxic mitochondria was measured polarographically from oxygen consumption. Results: Mitochondrial state 3 respiration decreased progressively with increased duration of anoxic incubation, while state 4 respiration increased progressively, The decrease in the ADP /O ratio with the duration of anoxic incubation indicated that the efficiency of oxidative phosphorylation decreased with increased duration of anoxia. As the duration of anoxic incubation increased, the amplitude and rapidity of the oxido-reduction change in NAD (P)H fluorescence decreased. The respiratory control ratio showed that the functional integrity of mitochondria was linearly correlated with the free [Ca2+] in mitochondrial matrix (r(2) = 0.977) and the total calcium concentration (r(2)= 0.934). The free (Ca2+] in mitochondrial matrix significantly decreased with increasing anoxic duration. Total mitochondrial calcium concentration also decreased with increasing anoxic duration (p < 0.01). Conclusions The supply of NADH was reduced during anoxic incubation Of isolated rat hepatocyte mitochondria, which resulted in impairment of mitochondrial respiratory function. The decrease in free (Ca2+] in the mitochondrial matrix with increased duration of anoxic incubation implies that calcium ions may be released into the cytosol during anoxia
In Situ Detection of Epstein-Barr Virus in Breast Cancer
No full textEpstein-Barr virus (EBV) is strongly associated with nasopharyngeal carcinoma and some lymphoepithelioma-like carcinomas from other anatomic sites. This study investigates the presence of (E) over dot BV in breast cancer. Immunohistochemistry for EBV proteins (EBV nuclear antigen-2 and latent membrane protein-1) and in situ hybridization for EBV-encoded small nuclear RNAs (EBER-1 and -2) were performed in 60 invasive breast cancers . None of the 60 breast cancer samples showed detectable EBV. These results suggest that EBV may not play a significant role in the etioloy of breast cancers in Taiwan. Published by Elsevier Science Ireland Ltd
Silicone Migration from Silicone-Injected Breasts: Magnetic Resonance Images
No full textInjection of liquid silicone into the breast was performed illicitly in the 1950s to 1960s and was subsequently prohibited. Many complications arise from silicone injection , and liquid silicone migration is a complication that has not been widely reported. The authors present magnetic resonance images of a patient with liquid silicone migration from the breast to the upper chest and lower neck. Breast ultrasonographic and mammographic findings are also presented for correlation
Investigation and Analysis of Surgical Manpower after Implementation of National Health Insurance.
No full textDr Robert King author
No full textDr. Robert King is pictured at the medical office on display at the Bishop Museum. King was the author of "A history of the practice of medicine in Manatee County, Florida", published in 1985. He was also a past president of the Manatee Historical Society
- …
