3,042 research outputs found
Switching cell morphologies by extracellular matrix proteins: fibronectin enhances dermal papilla cell aggregation by increasing cell-substratum adhesivity without compromising cell motility
No full textTo aggregate or not: fibronectin enhances dermal papilla cell aggregation by increasing cell-substratum adhesivity without abating cell motility
No full textProducing scalable inductive mesenchymal microtissues for hair follicle regeneration: effects of substratum and surface modification
No full textGenetic polymorphisms in CYP1A1 and GSTM1 predispose humans to PCBs/PCDFs-induced skin lesions.
No full text
High-pressure metamorphism in Taiwan: from oceanic subduction to arc-continent collision?
No full textFast initialization of level set method and an improvement to chan-vese model
No full textThe initialization is an important step in the level set method. However it is a computational time-consuming step. In order to speed up the level set evolution procedures, we first introduce a new initialization algorithm based on the vector distance transform, which propagates a vector with a position of the nearest object pixel instead of the scalar distance. A new sign map labeling method, based on the flood fill, is proposed to distinguish the inside and outside of the 2D closed active contour The active contour model proposed by Chan and Vese [1] can detect object whose boundaries are not necessarily defined by gradient. Our additional contribution to this paper is to present a further improvement to C-V model by replacing delta(phi) with \delphi\ to gain the global optimization. Finally, we illustrate the efficiency and performance of the proposed model by experimental results
Consciousness of language
No full textpublished_or_final_versionabstractChineseMasterMaster of Philosoph
Reverse phase protein array identifies novel anti-invasion mechanisms of YC-1
No full textYC-1 has recently been demonstrated to have potent anti-invasion and anti-metastatic activity in several cancer models, in addition to its anti-proliferation activity. However, the mechanism underlying its anti-invasion/anti-metastatic activity is largely unknown. Nasopharyngeal carcinoma (NPC) is a highly metastatic head and neck cancer in Southeast Asia. Here, we demonstrated that YC-1 inhibited invasiveness and proliferation of NPC cells, with the latter being accompanied by PARP cleavage, S-phase arrest and activation of Chk1/Chk2. We aimed at identifying novel anti-invasion mechanisms of YC-1 in NPC by a functional proteomic platform, the reverse phase protein array (RPPA). Our study revealed for the first time that multiple invasion-related signaling proteins (β-catenin, caveolin, Src and EGFR), as well as several growth-related proteins (AMPKα, phospho-acetyl-CoA carboxylase (p-ACC), HER-2 and mTOR), which were previously un-described signaling proteins altered by YC-1, were found to be down-modulated by YC-1 in NPC cells. We hypothesized that YC-1-mediated downregulation of these invasion proteins contributed to its anti-invasion activity in NPC cells. Overexpression of EGFR, activated Src or caveolin, but not β-catenin reversed the inhibitory effects of YC-1 on NPC cell invasion, with EGFR and activated Src having additional effects on rescuing NPC cells from YC-1-mediated growth inhibition. In summary, we have identified several novel anti-invasion mechanisms of YC-1 that could impact NPC, and possibly other cancers as well. © 2009.link_to_subscribed_fulltex
Involvement of YC-1 in extracellular signal-regulated kinase action in rat cremasteric muscle
No full textAbstractObjectivesThe nitric oxide (NO)–soluble guanylate cyclase (sGC) signalling pathway is attributed to the prevention of ischaemia–reperfusion (I/R)-induced leucocyte–endothelium adhesive interactions. YC-1 (3-(5′-hydroxymethyl-2′-furyl)-1-benzylindazole), a NO-independent sGC activator, has been shown to exert cardiovascular benefits, but its action on leucocyte–endothelium interactions remains unknown. In this study, the direct effect and the underlying mechanism of the anti-adhesive action of YC-1 have been examined in cremasteric microcirculation.MethodsRat cremaster muscle was subjected to 4 h pudic-epigastric artery ischaemia followed by 2 h reperfusion and intravital microscopy was used to observe leucocyte–endothelium interaction and to quantify functional capillaries in rat cremaster muscle flaps.Key findingsThe values for leucocyte rolling, adhering and transmigrating were 5.5-, 6.9- and 8.8-fold greater, respectively, in I/R than in sham-control animals. YC-1 treatment rescued functional capillary density and reduced leucocyte rolling, adhering and transmigrating in I/R injured cremaster muscles to levels observed in sham-controls. Interestingly, these effects were completely blocked by the MEK (extracellular signal-regulated kinase (ERK) kinase) inhibitor (PD98059) but not by sGC or protein kinase C inhibitors. Cotreatment of PD98059 with YC-1 caused a 3.3-, 7.5- and 8.3-fold increase in the values for leucocyte rolling, adhering and transmigrating, respectively, in postcapillary venules of I/R-injured cremaster muscle.ConclusionsThis study has indicated that the anti-adhesive and functional capillary density rescue properties of YC-1 were mediated predominantly by the activation of ERK but not sGC, although YC-1 was identified to be a sGC activator. A better understanding of the action of YC-1 on the microvasculature may help shed light on its therapeutic potential for cardiovascular disease.</jats:sec
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