23 research outputs found
Ataxin-2 Dysregulation Triggers a Compensatory Fragile X Mental Retardation Protein Decrease in Drosophila C4da Neurons
No full textDendrites require precise and timely delivery of protein substrates to distal areas to ensure the correct morphology and function of neurons. Many of these protein substrates are supplied in the form of ribonucleoprotein (RNP) complex consisting of RNA-binding proteins (RBPs) and mRNAs, which are subsequently translated in distal dendritic areas. It remains elusive, however, whether key RBPs supply mRNA according to local demands individually or in a coordinated manner. In this study, we investigated how Drosophila sensory neurons respond to the dysregulation of a disease-associated RBP, Ataxin-2 (ATX2), which leads to dendritic defects. We found that ATX2 plays a crucial role in spacing dendritic branches for the optimal dendritic receptive fields in Drosophila class IV dendritic arborization (C4da) neurons, where both expression level and subcellular location of ATX2 contribute significantly to this effect. We showed that translational upregulation through the expression of eukaryotic translation initiation factor 4E (eIF4E) further enhanced the ATX2-induced dendritic phenotypes. Additionally, we found that the expression level of another disease-associated RBP, fragile X mental retardation protein (FMRP), decreased in both cell bodies and dendrites when neurons were faced with aberrant upregulation of ATX2. Finally, we revealed that the PAM2 motif of ATX2, which mediates its interaction with poly(A)-binding protein (PABP), is potentially necessary for the decrease of FMRP in certain neuronal stress conditions. Collectively, our data suggest that dysregulation of RBPs triggers a compensatory regulation of other functionally-overlapping RBPs to minimize RBP dysregulation-associated aberrations that hinder neuronal homeostasis in dendrites. © The Korean Society for Molecular and Cellular Biology. All rights reserved.1
31.2 A 0.9V 28MHz Dual-RC Frequency Reference with 5pJ/Cycle and ±200 ppm Inaccuracy from -40°C to 85°C
No full textWireless sensor nodes in battery-powered internet-of-things (loT) applications require a stable on-chip frequency reference with low energy (<10 pJ / cycle) and high frequency stability (below ±300ppm). CMOS RC frequency references are promising due to their low-cost integration and high energy efficiency [1] –[5]. Conventional RC references, however, achieve only moderate accuracy (a few %) due to the large temperature coefficient (TC) of on-chip resistors [3]. First-order TC compensation can be achieved by combining resistors with complementary TCs [1], [2]. Although this is energy efficient (<6 pJ / cycle), it only partially compensates for the resistors’ high-order TCs, limiting the resulting accuracy to about ±500 ppm. Better accuracy (±100 ppm [4]) can be achieved by using the output of a digital temperature sensor (TS) to perform a polynomial correction of the phase-shift (μp,T) of an RC filter (Fig. 31.2.1). Alternatively, the phase-shifts (μp. and μN) of two RC filters with complementary TCs can be linearized (Tp. and T N ) and combined in the digital domain. Such dual-RC frequency references can also achieve good accuracy (±200 ppm [5]). However, both architectures employ an analog phase-domain ΔΣ modulator (Φ−ΔΣM) for each RC filter, which consumes significant energy (25pJ/cycle [4] and 107pJ/ cycle [5]) and area (0.3mm2[4]. and 1.65mm2[5]).Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.MicroelectronicsElectronic Instrumentatio
The Study on a Cooperative Education System for Logistics: the Case Study of International Program in Logistics Management Systems in Technology University of Eindhoven
No full textKorea needs to build world-class infrastructures that will shape the country into the logistics hub of the Northeast Asia. To achieve the goal, highly skilled and internationalized experts in the area of logistics are required. In Korea, however, there are no education programs that train qualified experts who have not only knowledge in logistics but also industrial and international experiences. This paper introduces a case study on education programs which cultivate such experts. An in-depth analysis was conducted on the global logistics program of Technical University of Eindhoven (TU/e) in the Netherlands. The global logistics program of TU/e is an international post master program in which several universities as well as famous companies such as Philips, Nokia, etc. are tightly involved. We analyzed the success factors of this program by investigating the roles of university, social community, and industry. Based on these success factors, some suggestions are made for establishing competitive logistics education programs in Korea.clos
A 0.9-V 28-MHz Highly Digital CMOS Dual-RC Frequency Reference With ±200 ppm Inaccuracy From -40 °C to 85 °C
No full textThis article presents an energy-efficient dual- RC frequency reference intended for wireless sensor nodes. It consists of a digital frequency-locked loop (FLL) in which the frequency of a digitally controlled oscillator (DCO) is locked to a temperature-independent phase shift derived from two different RC poly-phase filters (PPFs). Phase shifts with complementary temperature coefficients (TCs) are generated by using PPFs made from different resistor types (p-poly and silicided p-poly). The phase shift of each filter is determined by a zero-crossing (ZC) detector and then digitized by a digital phase-domain ΔΣ modulator ( Φ - ΔΣM ). The results are then combined in the digital domain via fixed polynomials to produce a temperature-independent phase shift. This highly digital architecture enables the use of a sub-1-V supply voltage and enhances energy and area efficiency. The 28-MHz frequency reference occupies 0.06 mm2 in a 65-nm CMOS process. It achieves a period jitter of 7 ps ( 1σ ) and draws 142 μW from a 0.9-V supply, which corresponds to an energy consumption of 5 pJ/cycle. Furthermore, it achieves ±200 ppm inaccuracy from −40∘C to 85 ∘C after a two-point trim.</p
Politics and Other Mistakes piece on a recent Superior Court preliminary injun
No full textPolitics and Other Mistakes piece on a recent Superior Court preliminary injunction blocking the section of Maine\u27s Clean Election Act that doubled the registration fees paid by lobbyists. The injunction came as a result of a lawsuit filed by the Maine Civil Liberties Union and the Maine Campground Owners Association. The piece also focuses on a Bates College study of Gov. Angus King\u27s political party allegiances, and U.S. senator Susan Collins Jan. 8 report to her constituents, which the author calls dull
Indian possession and playing: an American tradition from Tom Sawyer to today
No full textToni Morrison's deconstructionist analysis of the Africanist presence in nineteenth century texts is complemented by analysis of Nativist presence in the same time period and beyond. While the Africanist presence, or lack thereof, helped white authors express the venture for a democratic freedom, the Nativist presence has helped—and continues to help—white authors articulate an American identity which is romantic and distinctly their own, separate from Europe. A number of texts published in the nineteenth and twentieth centuries portray Native Americans in a paradoxical way: the figure is simultaneously the quintessential villain, savage and untrustworthy, and a romantic object of play, resistant to civilization and therefore a figure to be possessed and emulated. At the core of this paradoxical representation is Mark Twain's The Adventures of Tom Sawyer (1876). The novel's villain, Injun Joe, is the epitome of evil, yet the Native American is still the object of Tom's
imagination and infatuation. Even while Injun Joe is conveniently left to starve and die in an isolated setting, literally blocked from the rest of the civilization. Tom continues to "play Indian." Twain's novel appears at the transitional period between the nineteenth and twentieth centuries and it captures the ideologies of playing Indian and more specifically, the American ideologies within children's literature. This perpetuation of playing Indian lessons in children's literature is one which should be challenged and critiqued. The project will begin with an interrogation of the literary-historical roots of this cultural tradition, as found in Moby-Dick, The Last of the Mohicans, and Hohomok Then, Tom Sawyer will be employed as the transitional piece between the nineteenth and twentieth centuries, linking the
literary-historical accounts with more contemporary novels and films that exacerbate this trope, including Little House on the Prairie. The Catcher in the Rye, The Indian in the Cupboard, The Bean Trees, Disney's Pocahontas films, and Twentieth Century Fox's Night at the Museum. Rounding out the study is The Absolutely True Diary of a Part-Time Indian, which proves that the American fantasy continually permeates American children's literature and culture.M.A.Includes bibliographical referencesby Christine M. Pierucc
Relevance theory and the effect of literature on beliefs: the example of Injun Joe in Twain’s Adventures of Tom Sawyer
No full textTo what extent does literature affect our beliefs about the real world? Relevance theory offers new ways of exploring that old question. That is partly because relevance theory embraces the whole communications circuit: it tracks the communication of meaning from author via text to reader, rather than focusing on just one of those phases. It can also describe how unintended meaning can be inferred by readers. The question of the effect of literature upon beliefs is explored through one case study (Adventures of Tom Sawyer) and through various notions drawn from relevance theory: cognitive environments; contextual assumptions; implicatures; internal and external relevance; epistemic vigilance. It is argued that the evanescence or durability of any effects that literature may have upon readers’ beliefs can be investigated by combining those relevance-theoretic notions with ones drawn from certain other cognitive or literary-critical approaches: immersion; kinesis; perceptual simulation; tagging
