40 research outputs found
Prebiotic and Antiproliferation Effects of Arabinogalactan Isolated from Green Gram (Vigna radiata) and Its Hydrolysates.
Special Article: Legumes
Prebiotic and Antiproliferation Effects of Arabinogalactan
Isolated from Green Gram (Vigna radiata) and Its
Hydrolysates
Kiranmayi Ketha; Muralikrishna Gudipati*
Department of Biochemistry, CSIR- Central Food
Technological Research Institute, Mysuru, Karnataka,
India
*Corresponding author: G Muralikrishna
FAFST (I), FACCT(I) MNASc, Formerly Chief Scientist
(Scientist-G) & Formerly HOD, Professor AcSIR, Depart-
ment of Biochemistry, CSIR-Central Food Technological
Research Institute, Mysuru 570020, Karnataka, India.
Tel: +91-821-2516531 (R) Mobile (0) +91-9480382199
Email: [email protected]
Received: May 30, 2024
Accepted: June 25, 2024
Published: July 02, 2024
Annals of Agricultural & Crop Sciences
Volume 9, Issue 4 (2024)
www.austinpublishinggroup.com
Gudipati M © All rights are reserved
Citation: Ketha K, Gudipati M. Prebiotic and Antiproliferation Effects of Arabinogalactan
Isolated from Green Gram (Vigna radiata) and Its Hydrolysates. Ann Agric Crop Sci. 2024;
9(4): 1160.
Annals of Agricultural & Crop Sciences
Open Access
Abstract
An Arabinogalactan (AGP) derived from green gram and its hy-
drolysates (AGO, Arabinogalacto oligosaccharides) were screened
for their prebiotic potential and antiproliferation effects on co-
lon cancer cells (in vitro). Three lactobacilli strains namely L. aci-
dophilus, L. delbrucki and L. fermentum showed relatively better
response towards AGP and AGO as compared with other tested
strains with respect to defined prebiotic characteristics. The ac-
tivities of α-L-arabinofuranosidase (39 to 89.5 mU/mL) and β-D-
galactopyranosidase (19 to 49.5 mU/mL) in the culture broth in-
dicated the breakdown of AGP and AGO into constituent sugars
and their subsequent utilization for the growth of bacteria. AGP
and AGO exhibited dose dependent cytotoxic and anti-proliferative
effects on (p <0.001) Caco-2 cells with IC50 values of 150 and 500
μg/mL, respectively as estimated by MTT assay. The present study
demonstrated the prebiotic properties of AGP and AGO as well as
their ability to provide natural defence against colon cancer
Resistant Starch Degrading Enzyme from Lactobacilli Bacteria.
This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Isolation, purification and characterization of exopolysaccharide from Lactobacillus brevis NCDC01 grown on xylo-oligosaccharides.
This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Characterization of an Amylase from Native Finger Millet (Eleusine Coracana)
This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
A Study on the effect of Ph and Temperature on Xylanase Treatment with respect to the release of Xylo-Oligosaccharides from Wheat Bran
This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
A study on the effects of different salts on the growth of Lactobacillus brevis grown on wheat bran xylo-oligosaccharides.
This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
“Isolation of xylo-oligosaccharides from maize bran and determination of their antioxidant activity
This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Isolation of Xylooligosaccharides from Rice Bran and determination of their Antioxidant activity
This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Resistant starch degrading enzyme from Bifidogenic bacteria.
This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
