121 research outputs found
A comprehensive study on phytochemical characterization of Haplophyllum myrtifolium Boiss. endemic to Turkey and its inhibitory potential against key enzymes involved in Alzheimer, skin diseases and type II diabetes
No full text0000-0002-4435-8405WOS: 000338612500031The interest of medicinal plants as therapeutic agents against diseases such as Alzheimer, skin disorders and diabetes mellitus is growing. In order to investigate the influence of Haplophyllum myrufolium, four solvent extracts (petroleum ether, ethyl acetate, methanol and water) were screened for antioxidant potentials, anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glycosidase activity. Antioxidant effects were elucidated by different assays including free radical scavenging (ABTS, DPPH and NO assay), reducing power (FRAP and CUPRAC), phosphomolybdenum, beta-carotene/linoleic acid system and metal chelating. Total phenolic, flavonoid, flavanol, tannin and saponin contents in these extracts were also calculated. Generally, ethyl acetate extract showed the strongest antioxidant activity in these extracts. This activity may be related to a good total phenolic content. The total phenolic contents of these extracts ranged from 32.32 to 52.50 mg GAEs/g extract. Except for water extract, all extracts showed good inhibitory activities on cholinesterase, tyrosinase, amylase and glycosidase. Apolar extracts (petroleum ether and ethyl acetate) were more potent as enzyme inhibitors in comparison to polar extracts (methanol and water). These findings reveal that H. myrtifolium could be used a source of natural compounds for the management of oxidative damage, Alzheimer, skin disorders and diabetes mellitus. (C) 2014 Elsevier B.V. All rights reserved
Micromeria myrtifolia: Essential Oil Composition and Biological Activity
No full textWOS: 000475765700020Detailed chemical composition of the essential oil of Micromeria myrtifolia Boiss. & Hohen., as well as its antioxidant and enzymatic activity, was analyzed. The most abundant constituents of the oil were sesquiterpene hydrocarbons, beta-caryophyllene (40.8%) and alpha-copaene (17.9%), followed by oxygenated sesquiterpene, caryophyllene oxide (13.5 %). Antioxidant activity of the oil was tested via 8 different methods based on reduction of both radicals and stable metal cations. The oil of M. myrtifolia showed weaker, but still comparable activity with the known antioxidants, butylated hydroxyanisole and disodium edetate, that are used as positive controls. In addition, the essential oil of this aromatic plant species revealed strong inhibitory effects against tyrosinase and alpha-amylase. Present results suggest the use of the essential oil of endemic M. myrtifolia in a wide variety of applications in food and pharmacological industries.Ministry of Agriculture, Czech RepublicMinistry of Agriculture, Czech RepublicThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by project No. RO0418 (sustainable systems and technologies, improving crop production for higher quality of production of food, feed, and raw materials, under conditions of changing climate) funded by the Ministry of Agriculture, Czech Republic
Onosma ambigens: Phytochemical composition, antioxidant and enzyme inhibitory activity
No full text0000-0002-4435-8405WOS: 000554526900038Due to their medical importance, the number of studies on the enlightenment of the biological activities of Onosma species has been increasing over the years. In this study, besides the phytochemical composition of Onosma ambigens Lacaita (Boraginaceae), in vitro antioxidant, tyrosinase and alpha-amylase inhibitory activities of extracts obtained by using different solvents (ethyl acetate, methanol and water) were investigated. As a result of the spectrophotometric analysis, it was determined that methanol extract was rich in both phenolics (51.19 mg GAEs/g extract) and flavonoids (45.39 mg QEs/g extract). According to the results of liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) analysis, the major components of methanol extract were determined as rosmarinic acid (37670.55 mu g/g), luteolin 7-glucoside (15756.40 mu g/g), apigenin 7-glucoside (15080.34 mu g/g), luteolin (2289.77 mu g/g), apigenin (2240.38 mu g/g), chlorogenic acid (2121.92 mu g/g), vanillic acid (1179.66 mu g/g), hesperidin (1038.38 mu g/g), 4-hydroxybenzoic acid (961.97 mu g/g) and pinoresinol (631.61 mu g/g). It was found that methanol extract showed remarkable activity in almost all antioxidant activity tests (except ferrous ion chelating test). The activity of this extract in the phosphomolybdenum, reducing power [cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP)] and radical scavenging [on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)] assays were measured as 1.65, 0.95, 0.52, 1.86 and 1.45 mg/mL, respectively. Phenolic and flavonoid contents, especially chlorogenic acid, vanillic acid, luteolin 7-glucoside, hesperidin and rosmarinic acid, are thought to contribute significantly to the antioxidant activity of the extracts. In tyrosinase and a-amylase inhibitory activity tests, methanol (2.81 mg/mL) and ethyl acetate (2.64 mg/mL) extracts exhibited the best activity among the others, respectively. While it is thought that chlorogenic acid and rosmarinic acid may contribute to the tyrosinase inhibitory activity of methanol extract, it has been concluded that detailed chromatographic analyzes should be performed for detection of the compounds responsible for alpha-amylase inhibitory activity
Onosma pulchra: Phytochemical composition, antioxidant, skin-whitening and anti-diabetic activity
No full text0000-0002-4435-8405WOS: 000554526900026Researchers have discovered in recent years that some Onosma species have interesting biological/pharmacological activities. This study was conducted to investigate the antioxidant, tyrosinase and alpha-amylase inhibitor activity of ethyl acetate (EtOAc), methanol (MeOH) and water extracts of Onosma pulchra Riedl., an endemic plant species to the flora of Turkey. In the present study, the total phenolic and flavonoid contents of the extracts were also analyzed and the amounts of thirty-one different phytochemicals in the extracts were determined by using liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) technique. It was determined that the water extract shows the highest activity in 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), cupric reducing antioxidant capacity (CUPRAC), and ferrous ion chelating tests (3.90, 2.55, 2.20, and 1.23 mg/mL, respectively). Phosphomolybdenum and FRAP assays have resulted in the superiority of MeOH extract (1.98 and 1.02 mg/mL, respectively). In the tyrosinase inhibitory activity test, MeOH extract ranked first with an IC(50 )value of 2.47 mg/mL. In alpha-amylase inhibitory activity test, the activity of the EtOAc extract (IC50: 2.40 mg/mL) was found to be very close to acarbose (IC50: 1.02 mg/mL). As a result of total phenolic and flavonoid analysis, it was understood that MeOH extract is the richest extract in terms of both compound groups (26.93 mg GAEs/g extracts and 16.39 mg QEs/g extracts, respectively). It was followed by the water and EtOAc extracts, respectively. LC-ESI-MS/MS analysis showed that chlorogenic acid, luteolin 7-glucoside, hesperidin, hyperoside, rosmarinic acid, apigenin 7-glucoside, pinoresinol, luteolin and apigenin are found in high amounts in the extracts. It has been concluded that MeOH and water extracts exhibit considerable antioxidant activity and the reason for this is probably the phytochemicals with polar structure
Phenolic profile, antioxidant and enzyme inhibitory activity of the ethyl acetate, methanol and water extracts of Capparis spinosa L.
No full textIn this study, it was aimed to determine the phytochemical compositions and biological activities of ethyl acetate (EtOAc), methanol (MeOH) and water extracts obtained from the aerial parts of Capparis spinosa L. As a result of spectrophotometric analyzes, MeOH extract was found to be richer in terms of both phenolics and flavonoids compared to other extracts [81.45 mg GAEs (gallic acid equivalent)/g and 36.57 mg RE (rutin equivalent)s/g, respectively], while chromatographic analyzes showed that the extract in question contains a significant amount of hepseridin (72927.48 μg/g), quercetin (1335.88 μg/g), hyperoside (1227.73 μg/g), and 4-hydroxybenzoic acid (924.08 μg/g). Phosphomolybdenum, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, Cupric Reducing Antioxidant Power (CUPRAC) and Ferric Reducing Antioxidant Power (FRAP) reducing and ferrous ion chelating activity tests resulted in superiority of MeOH extract [371.0, 44.93, 56.46, 91.77, 52.61 mg TEs (trolox equivalent)/g and 14.85 mg EDTAEs/g, respectively]. On the other hand, EtOAc extract exhibited higher activity than other extracts in acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-amylase, and α-glucosidase inhibitory activity tests [3.29, 2.12 mg GALAEs (galanthamine equivalent)/g, 541.01 and 1584.20 mg ACEs (acarbose equivalent)/g, respectively]. The tyrosinase inhibitory activity test resulted in the superiority of MeOH extract [41.90 mg KAEs (kojic acid equivalent)/g]. A strong correlation was determined between the phenolic and flavonoid contents of the extracts and their antioxidant activities. © 2021 International Journal of Secondary Metabolit
The Antibacterial Activities of Piper nigrum L. Against Mastitis Pathogens and its Antioxidant Activities
No full text3rd Mediterranean Symposium on Medicinal and Aromatic Plants (MESMAP) - APR 13-16, 2017 - Girne, CYPRUSARSLAN, Ali/0000-0002-8722-591X; ceylan, olcay/0000-0002-4435-8405WOS: 000405924600006Objective / Purpose: Bacteria causes one of the most common types of chronic mastitis. The most common causative organisms of mastitis include: Staphylococci, Streptococci and coliforms. The scope of this work was to research the antibacterial effects of Piper nigrum extracts against mastitis pathogens, and its antioxidant capacity. Materials and Methods: In our study, 2 Staphylococcus aureus and 5 Coagulase Negative Staphylococcus were used for experiments. Additionally, Piper nigrum were collected from Mugla herbalists in Turkey. The plant extracts were tested by disc diffusion assay for antibacterial activity. The antioxidant activities of plant extracts were also determined by ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] method. Results: The methanol extract of plant showed maximum inhibition zone against 2 bacteria. MIC values of extracts are 6500 mu g/mL. The value found in ABTS method is highly effective (75.7%). Discussion/Conclusion: As a result, the in vitro studies indicate that the extracts of Piper nigrum have significant antibacterial and antioxidant activities. In addition to, the plant extracts could be used in treating mastitis caused by the test bacteria
Stachys germanica subsp. heldreichii (Boiss.) Hayek: Phytochemical analysis, antioxidant and enzyme inhibitory activities
No full textStachys species have traditionally been used by the people for thousands of years to treat various diseases. In this study, the chemical composition and enzyme inhibitory activities of the various extracts of Stachys germanica subsp. heldreichii (Boiss.) Hayek (Lamiaceae), which were isolated using solvents with different polarities, were investigated. Antioxidant activities of the extracts were analyzed by phosphomolybdenum, radical scavenging [on DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzthiazo-line-6-sulfonic acid)], reducing power [CUPRAC (cupric-reducing antioxidant capacity) and FRAP (ferric reducing antioxidant power)] and ferrous ion chelating tests, while tyrosinase and alpha-amylase were used for enzyme inhibitory activity assays. RACI (relative antioxidant capacity index) analysis showed that the ranking of the extracts was MeOH (methanol) > Water > EtOAc (ethyl acetate). In ABTS radical scavenging (2.26 mg/mL), phosphomolybdenum (1.68 mg/mL), CUPRAC (1.35 mg/mL) and FRAP tests (0.75 mg/mL), the MeOH extract showed the highest activity. However, the water extract exhibited the highest activity in DPPH radical scavenging and ferrous ion chelating tests. Tyrosinase inhibition potential of the MeOH extract was significant (IC50: 2.90 mg/mL). On the other hand, in a-amylase inhibition assay, EtOAc extract showed the highest activity (2.24 mg/mL). Amount of phenolics was higher in all extracts than flavonoids. MeOH extract was the richest one in phenolics and flavonoids. The major compounds of the extracts were chlorogenic acid and verbascoside. Pearson correlation analysis showed that these compounds were responsible especially for radical scavenging activity. Correlation between chemical composition and activity findings showed that verbascoside and apigenin contributed to tyrosinase and alpha-amylase inhibition, respectively. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved
Ziziphora taurica subsp. taurica: Analytical Characterization and Biological Activities
No full textThe Lamiaceae family comprises many flowering plants classified into about 236 genera. The genus Ziziphora is one of the well-known genera of this family and its species are important in different fields of pharmaceutical, chemical, traditional, and folk medicines. The phytochemicals present in Ziziphora include monoterpenic essential oils, triterpenes, and phenolic substances. The aim of this paper was to study the phytochemical profile of Ziziphora taurica subsp. taurica and compare and evaluate the biological activities of its ethyl acetate (ZTT-EtOAc), methanolic (ZTT-MeOH), and aqueous (ZTT-W) extracts based on their enzyme inhibition and antioxidant capacities. Determination of total phenolic (TPC) and total flavonoid (TFC) contents as well as biological activities were determined using spectrophotometric procedures. Subsequently, the individual phenolic compounds were detected by liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS/MS). In total, twenty-two different phenolic compounds were identified, including apigenin, ferulic acid, and luteolin which were the most common. ZTT-MeOH extract showed the best antioxidant activity, whereas ZTT-EtOAc extract was the most effective against tyrosinase and α-amylase. Ziziphora taurica subsp. taurica represents a potential source of natural compounds with positive effects on human health
Metabolite profiling and health benefits of Stachys cretica subsp. mersinaea as a medicinal food
No full textSarikurkcu, Cengiz/0000-0001-5094-2520; ceylan, olcay/0000-0002-4435-8405; Bahadori, Mir Babak/0000-0003-2556-4024WOS: 000460991700011The genus Stachys (Woundwort) is a widely used aromatic medicinal and functional food plant. Woundworts are consumed as vegetable, spice, and herbal decoction. Herein, different solvents and water extracts of Stachys cretica subsp. mersinaea were investigated for phytochemical contents, antioxidant and enzyme inhibition properties, and quantification of individual phenolic ingredients. Promising radical scavenging ability was recorded against DPPH (176 mg trolox equivalents/g extract) and ABTS (292 mg TEs/g extract) radicals. Also, high reducing power was observed in CUPRAC (256 mg TEs/g extract) and FRAP (236 mg TEs/g extract) assays. Extracts exhibited strong a-amylase (396 mg acarbose equivalents/g extract) and a-glucosidase (734 mg ACEs/g extract) inhibitory activity together with moderate inhibition of cholinesterases and tyrosinase. Reverse phase high performance liquid chromatography analysis indicated that hesperidin (25,770 mu g/g extract), quercetin (7740 mu g/g extract), kaempferol (5280 mu g/g extract), and benzoic acid (3080 mu g/g extract) were the most abundant phenolic compounds. The results showed that S. cretica subsp. mersinaea extracts have great capability for some applications as functional food, cosmetic, and pharmaceuticals
Chemical composition and biological activities of the essential oils of two endemic Nepeta species
No full textCavar Zeljkovic, Sanja/0000-0002-8534-3146; Sarikurkcu, Cengiz/0000-0001-5094-2520; ceylan, olcay/0000-0002-4435-8405WOS: 000448095400002The chemical composition, antioxidant activity and several enzyme inhibitions of the essential oils of two endemic Nepeta species, N. nuda subsp. glandulifera and N. cadmea were investigated for the first time. The major compounds of N. nuda subsp. glandulifera essential oil were geijerene (61.02%) and neointermedeol (6.07%). On contrary, essential oil of N. cadmea contained 70.94% of 4a beta,7 alpha,74 beta-nepetalactone. The oil of N. nuda subsp. glandulifera revealed better activity than the oil of N. cadmea against both reducing metal ions and radicals. Moreover, both oils have relatively weak but still noticeable activity against acetylcholinesterase and butyrylcholinesterase; weak activity against alpha-glucosidase, but quite high activity against alpha-amylase. On the other hand, both essential oils showed significant activity against tyrosinase. Presented results suggest that these two endemic species have strong potential to be used in food and pharmacological industries, and therefore they should to be investigated further
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