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    Correlation between the direct IgG MAR test (Mixed Antiglobulin Reaction test) and seminal analysis in men from infertile couples”

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    The direct IgG mixed antiglobulin reaction (IgG MAR) test was carried out in 395 male subjects from infertile couples, selected on the basis of their seminal characteristics (sperm concentration greater than 10 X 10(6)/ml, sperm motility greater than 10%); attempts were made to correlate results with the seminologic aspects of the ejaculate. A significant correlation was observed between a positive MAR test and spontaneous sperm autogglutination. No correlation was observed, however, between test positivity and concentration, motility and sperm morphologic features, the macroscopic aspects studied (viscosity, fluidification), or increased concentration of leukocytes

    “Sperm antibodies in infertile women: a comparison of 3 methods for handling the cervical mucus for extraction of immunoglobulins”.

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    ee procedures commonly employed in the extraction of soluble proteins from cervical mucus, for the application of the technique for the detection of sperm antibodies also in this biological fluid, were compared in order to establish which provided the highest yield. Studies were therefore carried out on 125 women with primary and secondary sterility characterized by good rheologic qualities of their mid-cycle cervical mucus and in presence of repeatedly poor PCTs, not justified by the seminal characteristics of their partners. The laboratory results and the statistical analysis have shown that as far as concerns IgG, liquefaction with bromelin and solubilization with Baker's buffer provide a better yield than ultracentrifugation; while bromelin alone has the best yield with IgA
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