1,721,073 research outputs found
Influence of granulosa cells and of different somatic cell types on mammalian oocyte development in vitro
No full textMouse antral oocytes regulate preantral granulosa cell ability to stimulate oocyte growth in vitro
No full textIn this study we evaluated whether mouse oocytes derived from early antral or
preovulatory follicles could affect the ability of preantral granulosa cells to
sustain oocyte growth in vitro. We found that early antral oocytes with a
diameter > or =75 microm did not grow any further during 3 days of culture on
preantral granulosa cell monolayers in vitro, while most of the oocytes with a
smaller diameter increased significantly in size. Similarly, about 65% of growing
oocytes isolated from preantral follicles grew when cultured on preantral
granulosa cells. By coculturing with growing oocytes fully grown early antral or
preovulatory oocytes, a small proportion (about 10%) of growing oocytes increased
in diameter, and changes in granulosa cell morphology were observed. Such effects
occurred as a function of the fully grown oocyte number seeded and were not
associated with a decrease in coupling index values. By avoiding physical contact
between antral oocytes and granulosa cells, the proportion of growing oocytes
undergoing a significant increase in diameter was about 36%. These results
indicate that fully grown mouse oocytes can control preantral granulosa cell
growth-promoting activity through the production of a soluble factor(s) and the
maintenance of functional communications with surrounding granulosa cells
“In vitro culture of ovarian follicles: A model for the study of regulative mechanisms of folliculogenesis and oogenesis”. (INVITED LECTURE)
No full textThe phosphorylation of a 21 kda polypeptide is differentially regulated in mouse oocytes during antral follicle development
No full textRole of antral follicle development and cumulus cells on in vitro fertilization of mouse oocytes
No full text
Stage-dependent modifications of amino acid uptake by antral and metaphase II mouse oocytes.
No full textModifications of leucine transport system of mouse oocytes have been studied
throughout Graafian follicle development and oocyte maturation. In contrast to
sheep oocytes (Moor and Smith, 1979), in the mouse kinetic constants and efflux
rate of leucine transport system did not vary in diestrus, proestrus, and
metaphase II (met II) oocytes. However, kinetics of leucine equilibration in
proestrus and met II oocytes was significantly slower than that found in diestrus
cells, and this may reflect a decreased availability of internal amino acids for
exchange
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