179,419 research outputs found
Forcipomyia piroskyi Cavalieri 1961
No full textpiroskyi Cavalieri, 1961b: 172 (female). Type locality: Argentina, Tierra del Fuego, Ushuaia, El Tristén, 54°43′59.24″S, 67°54′48.08″W. HT F (MLPA). Distr.: Argentina (Tierra del Fuego: type locality). Refs.: Cavalieri, 1961b: 172; Marino & Spinelli, 2001a: 105.Published as part of Spinelli, Gustavo R., Ronderos, María M., Ayala, Mahia M. & Díaz, Florentina, 2023, Catalog of the biting midges of Argentina (Diptera: Ceratopogonidae), pp. 1-83 in Zootaxa 5261 (1) on page 23, DOI: 10.11646/zootaxa.5261.1.1, http://zenodo.org/record/779505
Forcipomyia wygodzinskyi Cavalieri 1961
No full textwygodzinskyi Cavalieri, 1961a: 17 (female). Type locality: Argentina, Tierra del Fuego, Ushuaia, El Tristén, 54°43′59.24″S, 67°54′48.08″W. HT F (MLPA). Distr.: Chile, Argentina (Tierra del Fuego: type locality; PN Tierra del Fuego, Lapataia, 54°49′56.50″S, 68°33′45.20″W). Refs.: Cavalieri, 1961a: 17; Marino & Spinelli, 2001a: 106. delpontei Cavalieri, 1961b: 169. Type locality: Argentina, Tierra del Fuego, El Tristén, 54°43′59.24″S, 67°54′48.08″W. HT F (MLPA). New records: Tierra del Fuego: PN Tierra del Fuego, unnamed stream, 54º51′23.6′′S, 68º35′37.3′′W, 31 m; PN Tierra del Fuego, Sendero de la Baliza, unnamed stream at Bahía Lapataia, 54º51′36.4′′S, 68º33′43.7′′W, 8 m.Published as part of Spinelli, Gustavo R., Ronderos, María M., Ayala, Mahia M. & Díaz, Florentina, 2023, Catalog of the biting midges of Argentina (Diptera: Ceratopogonidae), pp. 1-83 in Zootaxa 5261 (1) on page 23, DOI: 10.11646/zootaxa.5261.1.1, http://zenodo.org/record/779505
Germogli di legalità
No full textNegli ultimi anni, la dirigenza comunista cinese ha optato per un rafforzamento del fazhi, ossia del governo della legge. La legalità socialista cinese costituisce la principale delle riforme sociopolitiche in atto, ma è anche foriera di profonde contraddizioni
Localized expression of Strim1, a novel member of the TRIM-containing family, guides the skeletal morphogenetic program of the sea urchin embryo
No full textThe building of the skeleton in the indirect
developing sea urchin embryo is a complex
morphogenetic process that is executed by the
Primary Mesenchyme Cells or PMCs (Ettensohn et
al, 1997; Wilt 2002). It is well known that the
PMCs acquire most of the positional and temporal
information from the overlying ectoderm for
skeletal initiation and growth (Armstrong 1993;
Cavalieri et al, 2003; Röttinger et al, 2008). In this
study, we analyze the function of a novel gene,
encoding for a tripartite motif-containing (TRIM)
protein denoted strim1, that adds up to the list of
genes constituting the epithelial-mesenchymal
signaling network.
We show that strim1 is expressed in ectoderm
regions adjacent to the bilateral clusters of PMCs.
Strim1 misexpression causes the number of PMCs
to double and leads to skeletal abnormalities. By
micromere transplantations, we establish that
skeletal defects depend upon strim1 misexpression
in ectoderm cells. Reciprocally, knock-down of
strim1 function abrogates PMC migration and
blocks skeletogenesis. Identical phenotype is
shown by chimeras in which strim1 function is
blocked selectively in the ectoderm. We also show
that clonal expression of strim1 into ectoderm cells
from knocked-down embryos restores the correct
skeletogenic program. Finally, we report that
strim1 triggers the expression of the ectodermspecific
gene pax2/5/8, and the PMC marker sm30
(an ectoderm signaling dependent gene).
We conclude that strim1 function is able to elicit
specific gene expression both in ectoderm cells and
PMCs to guide the biomineralization during
morphogenesis.
References
Armstrong, N, Hardin, J and McClay, DR (1993). Cell-cell
interactions regulate skeleton formation in the sea urchin embryo.
Development 119, 833-40.
Cavalieri, V, Spinelli, G and Di Bernardo, M (2003). Impairing Otp
homeodomain function in oral ectoderm cells affects skeletogenesis
in sea urchin embryos. Dev Biol 262, 107-18.
Ettensohn, CA, Guss, KA, Hodor, PG, and Malinda, KM (1997).
The morphogenesis of the skeletal system of the sea urchin embryo,
in: Collier JR (Ed.), Reproductive Biology of Invertebrates, vol. VII:
Progress in Developmental Biology, Oxford & IBH publishing Co.
Pvt. Ltd. New Delhi, Calcutta. 225-265.
Röttinger, E, Saudemont, A, Duboc, V, Besnardeau, L, McClay, D
and Lepage, T (2008). FGF signals guide migration of mesenchymal
cells, control skeletal morphogenesis and regulate gastrulation
during sea urchin development. Development 135, 353-65.
Wilt, FH (2002). Biomineralization of the spicules of sea urchin
embryos. Zoolog Sci 19, 253-61
Identification of a putative regulator of early-histone gene expression of Paracentrotus lividus
No full textDrosophila GAGA factor (GAF) is a nuclear protein, conserved along evolution, with multiple roles in gene
regulation, chromatin remodelling, Polycomb-dependent silencing, and insulator functions (1). GAF
recognizes and specifically binds GAGAG consensus DNA motif by its C2H2-type zinc-finger domain, and
interacts with other regulatory factors by its BTB/POZ domain. We have identified plGaga, a cDNA coding
for a putative GAF of the sea urchin P. lividus, which showed significant sequence similarity with
Drosophila and vertebrate GAFs. Real time RT-PCR revealed that the plGaga RNA is always present during
embryo development decreasing rapidly in abundance at the larval pluteus stage. We raised a specific
antibody against the sea urchin GAF and used it in whole-mount immunofluorescence assays, showing that
the protein was distributed predominantly to the nuclei of the whole embryo. By Chromatin
Immunoprecipitation experiments, we showed that the sea urchin GAF not only binds the sns5 insulator,
which is essential for the regulation of the sea urchin early-histone genes (2,3), but also to other GAGA
containing sequences in the early-histone gene cluster.
1 Matharu NK et al. 2010 JMB 400 (3): 434–447
2 Melfi R et Al. 2001 JMB (1); 304:753-63
3 Cavalieri V et al. 2013 Nucleic Acids Res 37: 7407-741
P. Franchi de Cavalieri et J. Lietzmann, Specimina codicorum grœcorum Vaticanorum.
No full textBousquet R. P. Franchi de Cavalieri et J. Lietzmann, Specimina codicorum grœcorum Vaticanorum. . In: Échos d'Orient, tome 14, n°86, 1911. pp. 61-62
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