86,552 research outputs found
Urban assemblage, repair, ragionevolezza
No full textThe contribution is conceived as a conversation between Filippo Cattapan and Angelo Lunati, respectively in the guise of theorist and architect, on the possibility of a rational architecture that is not based solely on technical and material issues, but also and above all on disciplinary ones, of formal, urban and compositional nature.
A critical and theoretical reflection will be accompanied by a timely exchange on a series of projects by onsitestudio, Milanese practice of which Angelo is partner together with Giancarlo Floridi. In particular, the following case studies will be discussed: the Sassuolo football centre as an example of construction linked to the building tradition of its territory, the Garibaldi block in Milano as a prototype of urban transformation and strategic prefabrication, the restoration of the former Ansaldo factories as an adaptive reuse of industrial space, the design of the new BEIC library as public monument and passive energy device.
If the contemporary condition of scarcity undoubtedly calls for a substantial technical rationalisation of building practices, this must not become, in our opinion, the first horizon of an architecture. The current tendency to direct design discourses on sustainability appears needed as much as risky, as it seems to lead towards a new disciplinary hegemony of technique. This reductive form of technical rationalism is exactly what post-war architettura razionale has struggled to escape.
In opposition to a mere aesthetics of sustainability, that is often rendered with an appearance of bricolage, we will propose to revisit once again a broader tradition of thought, a bricoleur rationality based on the notions of context, urbanity and continuity. It is the tradition of Adolf Behne and James M. Richards, but also of Aldo Rossi and Oswald Mathias Ungers, of Colin Rowe and thus of Isaiah Berlin and Karl Popper. It is also the natural field of a certain Milanese rationalism, we might perhaps say pragmatism, on which onsitestudio has reflected at length and in whose trajectory they have tried to place their work
Estrazione del DNA per ricerca OGM in barrette energetiche
No full textL'estrazione del DNA dai campioni di barrette energetiche è stata ottenuta utilizzando una metodica specifica per la metrice, alternativa a quella del metodo convenzionale CTAB. Questa metodica specifica prevede la lisi del campione mediante un tampone <tris-HCl contenente proteinasi K e il recupero del DNA per precipitazione utilizzando allo scopo sodio acetato e ispopropanolo. Il DNA estratto è stato poi sottoposto a due Nested PCR, una per amplificare il gene della lecitina specifico per la solia e l'altra per amplificare il gene eterologo della soia transgenica Roundup Ready (TM). Il DNA amplificato è stato infine sottoposto ad elettroforesi su gel di agarosio. Il risultato ha messo in evidenza come il metodo di analisi proposto sia da considerare un'alternativa utile nei casi in cui si dubiti che il metodo convenzionale possa fornire risultati attendibili.The paper proposes a specific method for GMO detection in energetic bar. The extraction method is based upon the use of a buffer, TRIS-HCl, containing Proteinase K. The DNA extracted is subjected to two Nested PCR: the first one to amplify the Soy specific lectin gene; the second one to amplify the heterologous gene of transgenic soy. The proposed method results particularly efficient in case of food preparation with complex matrices
Innovazioni in PCR per l'identificazione di OGM in lecitine di soia
No full textIl lavoro svolto su una larga serie di campioni commerciali di lecitina di soia ha consentito di dedurre considerazioni tali da proporre per le verifiche OGM di questa matrice un opportuno accoppiamento di tecniche fino ad oggi non considerate in coordinazione. La proposta deriva dalla combinazione razionale di lavori di A. Zimmerman et al., E. Koppler et al., R. Meyer
International design competition for the redevelopment of St. Amandus Church into a library and liturgical space in Strombeek-Bever (Belgium)
No full textPiù che un progetto tradizionalmente concepito, la proposta delinea un processo di ricerca sotto forma di riflessione progettuale.
Tale processo inizia con una domanda chiave: come possiamo progettare, in occasione della trasformazione di una chiesa in una biblioteca pubblica, uno spazio che sia accogliente per tutti, ma che allo stesso tempo si inserisca nella storia materiale della città e dei suoi monumenti?
Attraverso la ricerca connessa all’idea di “design by research”, il progetto ha cercato di capire quali funzioni dovessero essere collocate all'esterno della chiesa (neutrali) e quali all'interno (non neutrali). Questo principio si è a sua volta sovrapposto a una questione tecnica fondamentale: quali stanze dovrebbero essere regolarmente riscaldate e quali no?
La biblioteca fornisce una nuova facciata urbana alla piazza. Non vuole essere una semplice estensione della chiesa, ma una nuova interfaccia con lo spazio pubblico
Surgery for Residual Inferior Left-to-Right Atrial Shunt
No full textWe report the case of three female patients who were scheduled for surgical correction of residual left-to-right shunt after initial repair of sinus venosus atrial septal defect (SV-ASD) during childhood. After excluding the possibility of an hemodynamic intervention, all three patients underwent a successful surgical closure through a right mini sub-axillary approach by using total peripheral cannulation for cardiopulmonary bypass and leaving the inferior vena cava completely un-snared allowing for an optimal visualization of the residual atrial septal communication and avoiding extensive dissection of mediastinal structures
Preoperative Transcatheter Diagnosis of Right Atrial Hemangioma
No full textA 34-year-old man with unremarkable past medical history presents with night sweats and a recent diagnosis of intracardiac mass. The initial diagnostic workup did not provide a definitive diagnosis, so a cardiac biopsy under intracardiac echocardiography guidance was performed, revealing a hemangioma, which was then successfully resected. (Level of Difficulty: Advanced.
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Spermine-mediated Casein Kinase II-uptake By Rat-liver Mitochondria
No full textSpermine, ubiquitous intracellular polyamine, is able to promote the transmembrane translocation of casein kinase CKII through the outer membrane of rat liver mitochondria and its binding to more internal mitochondrial structures. These findings suggest that spermine may play a critical role in regulating the subcellular distribution of casein kinase CKII
[Newspaper Clipping: Author Claims Evidence of Second JFK Assassin #1]
No full textNewspaper article titled "Author Claims Evidence of Second JFK Assassin." The article states that author Richard J. Whalen concluded "that there is circumstantial evidence to support the theory of a second assassin in the shooting of President John F. Kennedy.
- …
