1,721,054 research outputs found
Automated flow cytochemistry in measuring chronic lymphocytic leukemia- and healthy adult cells
No full textLymphocyte size measured by automated flow cytochemistry mayhave prognostic implication in chronic B-lymphocytic leukemia
Comparative results of the mitogenic effects of polyclonal B-cell activators in different stages of chronic lymphocytic leukemia
No full textIn order to correlate the mitogenic response pattern of cultured lymphocytes with different polyclonal activators (PMA, LPS, SA, PHA-M) and clincal stage of the disease, the expression of cytoplasmic Ig, secr tion of Ig, modal cell volume, la antlgen, acid phosphatase activity and mitotic ndex were measured following culture twenty three consecutive patients wlth B-cell.
The results showed that patients with advanced Ral clinical stages (III and IV) presented a more heterogenous stimulatory pattern, depending on the severity of their disease and mitoyen employed. Furthermore, whlle PHA-M appeared to be the most efflcient stimulator in the initial Rai stages of CLL, showing a close correlatîon with the high percentage of blood T celle, LPS mitogen was able to induce an optimal dlfferential pattern in terminal Rai stages only. However, in spite of the differences observed in the patients at different stages of the disaease, a consistent number of stlmuiated celle was found wlth all tested mitogens. Thls report suggests that a global evaluation of the attivation of the neoplastlc B•cell clone in B-CLL, should be achieved by the use of multiple polyclonal B-celt activators
Potential clinical applications of rhGM-CSF in acute myeloid leukemia based on its biological activity and receptor modulation
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Cytogenetic aspects of B- cell chronic lymphocytic leukemia: their correlation with clinical stage and different polyclonal mitogens
No full textBlood lymphocytes from 23 patients with B-cell chronic lymphocytic leukemia were stimulated with different mitogens (lipopolysaccharide from E coli, phorbol myristate acetate ester, Staphylococcus aureus, and phytohemagglutinin M form). Functional properties of stimulated cells (blastic transformation, mitotic index, cIg production, Ig secretion, and acid phosphatase positivity) were evaluated and correlated with the stage of disease and chromosomal findings. Early stages of the disease are characterized by a relatively homogeneous response to polyclonal B-cell and activators and by a restricted number of chromosomal aberrations. Advanced stages show a more heterogeneous pattern of response and a higher incidence of abnormal karyotypes suggesting an involvement of various subclonal lines
Significato dell'analisi citogenetica e citogenetico-molecolare nella diagnosi e nella prognosi delle neoplasie ematologiche. Cytogenetics and molecular cytogenetics in diagnosis and prognosis of haematological malignancies
No full textConventional cyogenetics analysis along with molecular cytogenetics techniques (e. g. fluorescence in situ hybridization, FISH) gratly contributed to the identification of recurrent chromosomal abnormalilities in hematologic malignancies, often related to clinical and pathological features. Thus, cytogentic analysis could be considered an essential tool to correctly define the diagnosis and the prognostic risk in many tum ors. The knowledge of chromosomal and molecular abnormalities led to the elucidation of ethiopathogernetic mechanism which cause a loss of control in self-renewal, apoptosis and proliferation and gave the basis for the development of gene-targeted therapy
Report on the fifth International Workshop on Human Leukocyte Differentiation Antigens
No full textThis paper represents a detailed description of the results and basic studies presented at the 5th International Workshop
on Leukocyte Differentiation Antigen held in Boston on November 3-7, 1993. More than 500 institutions worldwide participated in the
Workshop study groups, and approximately 1450 antibodies were investigated, allowing the identification of 48 new CD clusters and the
redelineation of 14 previously clustered molecules . All the monoclonal antibodies (mAbs) were analyzed by flow cytometry and the data recorded in a Leukocyte Differentiation Antigen Database (LDAD), which represents the source of the information found in this workshop
report. A more extensive account of this Workshop will be published by Oxford University Press before the end of 1994.
The workshop was divided into 11 sections and the workshop organizers were the following
scientists: Stuart Schlossman, Chairman of the Meeting, Laurence Boumsell (T-cell section),
Wally Gilks (Statistical analysis), John Harlan (Endothelial cells), Tadamitsu Kishimoto
(Cytokine receptors), Chikao Morimoto (Activation antigens), Jerome Ritz (NK antigens), Stephen Shaw (Cross-lineage blind panel), Timothy Springer (Adhesion section), Roy Silverstein (Platelets), Thomas Tedder (B cells), Robert Todd (Myeloid cells)
Does a relationship exist between neutrophil myeloperoxidase deficiency and the occurence of neoplasms ?
No full text36 unrelated individuals with neutrophil MPO deficiency, (10 totally MPO deficient) were found on screening a population of 148,000 subjects. A further 2 subjects with total and 22 with partial MPO deficiency were identified through family studies. The assessment of neutrophil function, i.e., peroxidase activity, superoxide anion generation, microbicidal activity towards fungi and bacteria, and locomotor behaviour, was carried out in 10 subjects with total and 4 with partial MPO deficiency. We found that the enzyme defect is associated with a marked impairment in the killing of both S. aureus and C. albicans, without affecting microbicidal activity against S. faecalis. There appears to be a high incidence of malignancy in patients with complete MPO deficiency, suggesting a relationship between a defective MPO system and neutrophil-mediated tumor cell cytotoxicity
Caratterizzazione degli epitopi del CD34 ed analisi dei recettori per fattori di crescita nelle cellule staminali emopoietiche
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