1,721,018 research outputs found
Effects of Er:YAG laser and ultrasonic treatment on fibroblast attachment to root surfaces: an in vitro study
No full textBACKGROUND:
The aim of this study was to analyze the effects of erbium-doped:yttrium, aluminum, and garnet (Er:YAG) laser and ultrasonic treatment on fibroblast attachment to periodontally diseased root surfaces.
METHODS:
Thirty single-rooted human periodontally involved teeth were included in this study. A total of 60 specimens were obtained from all selected teeth and were randomly assigned to the following three groups: group A, untreated control group; group B, ultrasonic group; and group C, Er:YAG laser at 160 mJ/pulse at 10 Hz group. All of the specimens were incubated in petri dishes with fibroblast suspension and observed by scanning electron microscopy.
RESULTS:
Laser-treated specimens showed a significantly higher cell density number, with a mean+/-SD of 3,720+/-316 cells/mm2. The ultrasonically treated group showed a lower cell density number, with a mean+/-SD of 658+/-140 cells/mm2. The untreated control group showed the lowest cell density number, with a mean+/-SD of 130+/-80 cells/mm2. Differences between all groups were significant (P<0.0001).
CONCLUSION:
The results of the study indicate that untreated control surfaces and ultrasonically treated surfaces exhibited a significantly lower number of attached cells compared to laser-treated specimens, which showed a significantly higher cell density number
Controlling the lateral distribution and alignment of human gingival fibroblasts by micropatterned polysaccharide surfaces
No full text
Effects of molecular weight and surface functionalization on surface composition and cell adhesion to hyaluronian coated titanium.
No full textEffects of molecular weight and surface functionalization on surface composition and cell adhesion to Hyaluronan coated titanium
No full textThis paper describes the effect of surface functionalization on surface composition and cell adhesion to titanium samples by high and low molecular weight Hyaluronan (HA). HA was covalently linked to aminated Ti surfaces obtained by two different surface functionalization techniques, that is polyethyleneimine (PEI) adsorption and deposition from allylamine plasma. The two approaches yield very different surface densities of available amino groups, affecting this way the number and frequency of surface-HA bonds and the configurational freedom of the latter. Results of cell adhesion test are dependent on the surface functionalization approach adopted, low molecular weight HA coupled to PEI functionalized Ti does not yield the same degree of resistance to cell adhesion found on other samples. These results indicate that the details of the surface functionalization step are crucial for surface engineering of implant devices by biological molecule
Plasma of argon accelerates murine fibroblast adhesion in early stages of titanium disk colonization
No full textPurpose: This study was conducted to analyze how a cleaning treatment using plasma of argon would
affect fibroblast growth on titanium disks at different time points to determine whether this treatment could
enhance soft tissue healing around titanium dental implant abutments. Materials and Methods: Sixty sterile
disks made of machined grade 5 titanium were divided into two groups; 30 disks were left untreated (control)
and 30 were cleaned using plasma of argon (test). To simulate clinical conditions during soft tissue healing
around titanium abutments, both groups were immersed in a culture of murine fibroblasts (L929) for 2, 8,
or 48 hours. After preparation, they were stained using 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI)
to label the cellular nuclei and fluorescent phalloidin to label the cellular bodies. The nuclei were counted,
and cellular bodies were analyzed with fluorescent microscopy and imaging analysis software. Analysis was
performed at the three different time points. Results: Fibroblast adhesion for the test group was statistically
significantly greater versus the control group at 2 and 8 hours but not at 48 hours. At 2 and 8 hours, the
cellular bodies in the test group appeared flatter and more spread out, revealing more advanced cellular
adhesion, compared to the cells observed in the control group. At 48 hours, the test and control specimens
were nearly indistinguishable. Conclusion: The removal of organic and inorganic contaminants from the
surfaces of titanium disks using plasma of argon accelerated fibroblast adhesion in the early stages of
colonization (2 to 8 hours). This effect disappeared after 48 hours as a result of saturation. Clinically,
abutment cleaning using plasma of argon might positively affect soft tissue healing in early stage
SURFACE FUNCTIONALIZATION OF 3D GLASS-CERAMIC POROUS SCAFFOLDS FOR ENHANCED MINERALIZATION IN VITRO
Full text linkBone reconstruction after tissue loosening due to traumatic, pathological or surgical causes is in increasing demand. 3D scaffolds are a widely studied solution for supporting new bone growth. Bioactive glass-ceramic porous materials can offer a three-dimensional structure that is able to chemically bond to bone. The ability to surface modify these devices by grafting biologically active molecules represents a challenge, with the aim of stimulating physiological bone regeneration with both inorganic and organic signals. In this research work glass ceramic scaffolds with very high mechanical properties and moderate bioactivity have been functionalized with the enzyme alkaline phosphatase (ALP). The material surface was activated in order to expose hydroxyl groups. The activated surface was further grafted with ALP both via silanization and also via direct grafting to the surface active hydroxyl groups. Enzymatic activity of grafted samples were measured by means of UV-vis spectroscopy before and after ultrasonic washing in TRIS-HCl buffer solution. In vitro inorganic bioactivity was investigated by soaking the scaffolds after the different steps of functionalization in a simulated body fluid (SBF). SEM observations allowed the monitoring of the scaffold morphology and surface chemical composition after soaking in SBF. The presence of ALP enhanced the in vitro inorganic bioactivity of the tested materia
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