1,721,740 research outputs found
Establishing micropropagation protocols for new strawberry (Fragaria × ananassa) breeding lines
No full textMicropropagation remains an important tool for strawberry nursery production aimed at improving plant sanitary and genetic quality. To have this result without the risk to induce genetic and phenotypic variability efficient micropropagation, protocols for each genotype must be developed, mainly based on the use of proliferation substrates with low cytokinin content and a controlled number of subcultures. In addition, the in vivo bud sterilization is one of the critical steps for the establishment of high-quality proliferative explants, as well as acclimatization conditions for the efficient in vivo transfer of the micropropagated plants. Micropropagation technology is of high support for the fast development of new breeding material with high interest for the market. The aim of this study was to verify the different response to the in vitro establishment and proliferation of different strawberry (Fragaria × ananassa) genotypes derived from the breeding program developed at Ancona, University (Italy). The response to a standard sterilization protocol was tested on lateral and terminal buds of runners harvested from in vivo strawberry plants, in two different periods (June and September), and the genotype effect on in vitro explant proliferation at low-cytokinin concentration, was studied. The goal was to obtain healthy mother plants for starting in vivo propagation of new breeding selections
Investigating the composition and the anti-proliferative mechanism of a strawberry (Fragaria × ananassa) methanolic extract on in vitro murine breast cancer cells
No full textThe aim of this study was the evaluation of the effects of strawberry extracts treatment on two in vitro models of murine breast cancer cell line, trying to detect a specific pathway (AMPK) through which strawberries exert their anticancer activity. Firstly, a characterization of the sensorial and nutritional quality of 'Alba' strawberry cultivar was performed. Then, the anticancer activity of methanolic extract from 'Alba' on two murine cancer cell lines, N202/1A (with high levels of HER2/neu oncogene) and N202/1E (with low levels of HER2/neu oncogene) was evaluated after 48 h of treatment through the expression of several proteins related to apoptosis, autophagy, metastasis, oxidative status, mitochondrial functionality, oncogene expression and AMPK pathway. This study demonstrated that a well characterized strawberry extract possessed an antiproliferative effect on cancer cells, through the induction of apoptosis and oxidative stress. At the same time, a relation between the AMPK pathway and the anticancer effects of strawberries was demonstrated
Variation of polyphenol and vitamin C fruit content induced by strawberry breeding
No full textThe modification of the lifestyle in modern society has underlined new crucial aspects from the nutritional point of view. Among fruits, strawberry received in the last years an increasing attention, and a growing number of scientific evidence demonstrated how a short-or a long-term consumption of strawberries could be beneficial for the consumers. The nutritional properties of strawberry depend on the amount and the profile of the bioactive and antioxidant compounds it contains (e.g., polyphenols and vitamins). The recent aim of different breeding programs is to select new strawberry cultivars having fruit with high concentrations of antioxidant compounds. In this study, 14 commercial cultivars and 5 advanced selections from the UNIVPM-D3A breeding program have been deeply evaluated for their nutritional quality for three consecutive years, from 2016 to 2018. The concentration of the main bioactive compounds, such as anthocyanins, phenolic acids, and vitamin C, were measured through HPLC-UV. Results demonstrate the high variability in bioactive compounds composition among cultivars and breeding materials and the possibility to generate new cultivars producing fruit with high contents of polyphenols and vitamins, stable at the different cultivation cycles, to be labelled with a compositional claim
Yield and fruit quality of strawberry cultivars grown in organic farming in the mid-Adriatic area
No full textOrganic fruit and vegetable productions in Marche Region, the main Italian Region of the mid-Adriatic area, are still rising. The interest for the production of organic strawberries is also increasing and brings about the need to identify strawberry cultivars with higher adaptability to open field and protected organic cultivation in this area. With this aim, thanks to a collaboration started between D3AUNIVPM and the Regional Extension Service Agency (ASSAM), a two years experimental trial was set, comparing sixteen cultivars of strawberries; thirteen of these cultivars were short day cultivars ('Alba', 'Aprica', 'Asia', 'Brilla', 'Clery', 'Cristina', 'Garda', 'Joly', 'Madeleine', 'Premy', 'Romina', 'Sibilla', and 'Syria'), while three were day neutral ('Albion', 'Malga' and 'Monterey'). Among these cultivars, only four were already recommended for strawberry integrated crop production in the Marche Region, while all the other were identified from different national and international programs. The experimental trial, set in 2014, was prepared following a randomized design with three blocks of sixteen plots, of twenty plants each, for both cultivation conditions (open field and protected-Veronese tunnel). At harvest time, data on plant and fruit susceptibility to fungal and pest diseases were detected and vegetative (vigor of the plant, number of crowns), productive (total plant production, total discard for rot and deformed fruit and fruit average weight) and quality (texture, color, content sugars and titratable acidity) parameters were measured. The results showed a significant difference in adaptability, yield and satisfactory quality of some cultivars. Of greatest interest resulted 'Romina', 'Sibilla' and 'Cristina', as short day, and 'Monterey' as day neutral. The 'Veronese tunnel' resulted as a flexible and easy way to manage protected cultivation system that helped to increase yields of different cultivars, highlighting how this technique is essential for the success of strawberry organic production in this area
Morphological, nutraceutical and chemical characterization of globe artichoke (cynara cardunculus l. var. scolymus (l.) fiori) landraces typically cultivated in marche area
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