63,340 research outputs found
Structure-activity relationship on fungal laccase from Rigidoporus lignosus: a Fourier-transform infrared spectroscopic study
The structure and thermal stability of a laccase from Rigidoporus lignosus (Rl) was analysed by Fourier-transform infrared (FT-IR) spectroscopy. The enzyme was depleted of copper atoms, then part of the apoenzyme was re-metalled and these two forms of the protein were analysed as well. The enzymatic activity, lost by the removal of copper atoms, was restored in the re-metalled apoenzyme and resulted similar to that of native protein. The infrared data indicated that the enzyme contains a large amount of beta-sheets and a small content of alpha-helices, and it displayed a marked thermostability showing the T(m) at 92.5 degrees C. The apoenzyme and the re-metalled apoenzyme did not show remarkable differences in the secondary structure with respect to the native protein, but the thermal stability of the apoenzyme was dramatically reduced showing a T(m) close to 72 degrees C, while the re-metalled protein displayed the T(m) at 90 degrees C. These data indicate that copper atoms, beside their role in catalytic activity, play also an important role on the stabilisation of the structure of Rl laccase. About 35% of the polypeptide chain is buried and/or constitutes a particular compact structure, which, beside copper atoms, is probably involved in the high thermal stability of the protein. Another small part of the structure is particularly sensitive to high temperatures and it could be the cause of the loss of enzymatic activity when the temperature is raised above 45-50 degrees C
Cambria-Jackson Mine cars
Date scanned: 2002-6-20.USBM #64566; Dumping roll over-type cars at shaft station, using pipe bar. Cambria-Jackson Mine, The Cleveland-Cliffs Iron Company, Negaunee, Mich. - M. S. Petersen - Sept. 1946.Held in the Russell L. and Lyn Wood Mining History Archive, Arthur Lakes Library, Colorado School of Mines.Donor: United States Bureau of Mines.The first iron ore in the Lake Superior region was found on the site of the Jackson Mine, leading to discoveries of the extensive Lake Superior iron ore deposits in the Marquette Range. The Jackson pit mine was opened in 1848 by prospectors from Jackson, Michigan who formed the Jackson Mining Company. The Cambria Mine, which opened in 1874, was eventually included in the property. The Cambria-Jackson Mine was consolidated with the Mather Mine, which began shipping ore in 1943. These properties were operated by the Cleveland-Cliffs Iron Company. The Mather Mine was the last underground iron mine operating in Michigan and finally closed in 1979. Cleveland-Cliffs Iron Company was formed with the merger of two major iron mining companies on Michigan's Marquette Range in 1891. The Company had a number of mines operating in the Upper Peninsula by the outbreak of World War I. By the 1940s the high grade iron ores mined underground were becoming depleted. The Company developed a process to concentrate low grade ores into iron ore pellets in the 1950s, and C. C. I. C.'s last underground iron mine closed in 1979
Cambria-Jackson Mine hoisting cable
Date scanned: 2002-5-23.USBM #60198; Greasing hoisting cable using compressed-air gun. Cambria-Jackson Mine, The Cleveland-Cliffs Iron Company, Negaunee, Michigan. - M. S. Petersen - May 1945.Held in the Russell L. and Lyn Wood Mining History Archive, Arthur Lakes Library, Colorado School of Mines.Donor: United States Bureau of Mines.The first iron ore in the Lake Superior region was found on the site of the Jackson Mine, leading to discoveries of the extensive Lake Superior iron ore deposits in the Marquette Range. The Jackson pit mine was opened in 1848 by prospectors from Jackson, Michigan who formed the Jackson Mining Company. The Cambria Mine, which opened in 1874, was eventually included in the property. The Cambria-Jackson Mine was consolidated with the Mather Mine, which began shipping ore in 1943. These properties were operated by the Cleveland-Cliffs Iron Company. The Mather Mine was the last underground iron mine operating in Michigan and finally closed in 1979. Cleveland-Cliffs Iron Company was formed with the merger of two major iron mining companies on Michigan's Marquette Range in 1891. The Company had a number of mines operating in the Upper Peninsula by the outbreak of World War I. By the 1940s the high grade iron ores mined underground were becoming depleted. The Company developed a process to concentrate low grade ores into iron ore pellets in the 1950s, and C. C. I. C.'s last underground iron mine closed in 1979
Cambria-Jackson Mine hoisting cable
Date scanned: 2002-5-23.USBM #60199; Greasing hoisting cable using compressed-air gun. Cambria-Jackson Mine, Cleveland-Cliffs Iron Company, Negaunee, Michigan. - M. S. Petersen - May 1945.Held in the Russell L. and Lyn Wood Mining History Archive, Arthur Lakes Library, Colorado School of Mines.Donor: United States Bureau of Mines.The first iron ore in the Lake Superior region was found on the site of the Jackson Mine, leading to discoveries of the extensive Lake Superior iron ore deposits in the Marquette Range. The Jackson pit mine was opened in 1848 by prospectors from Jackson, Michigan who formed the Jackson Mining Company. The Cambria Mine, which opened in 1874, was eventually included in the property. The Cambria-Jackson Mine was consolidated with the Mather Mine, which began shipping ore in 1943. These properties were operated by the Cleveland-Cliffs Iron Company. The Mather Mine was the last underground iron mine operating in Michigan and finally closed in 1979. Cleveland-Cliffs Iron Company was formed with the merger of two major iron mining companies on Michigan's Marquette Range in 1891. The Company had a number of mines operating in the Upper Peninsula by the outbreak of World War I. By the 1940s the high grade iron ores mined underground were becoming depleted. The Company developed a process to concentrate low grade ores into iron ore pellets in the 1950s, and C. C. I. C.'s last underground iron mine closed in 1979
Cambria-Jackson Mine compressed-air gun
Date scanned: 2002-5-23.USBM #60200; Compressed-air gun for greasing hoisting cables. Cambria-Jackson Mine, Cleveland-Cliffs Iron Company, Negaunee, Michigan. - M. S. Petersen - May 1945.Held in the Russell L. and Lyn Wood Mining History Archive, Arthur Lakes Library, Colorado School of Mines.Donor: United States Bureau of Mines.The first iron ore in the Lake Superior region was found on the site of the Jackson Mine, leading to discoveries of the extensive Lake Superior iron ore deposits in the Marquette Range. The Jackson pit mine was opened in 1848 by prospectors from Jackson, Michigan who formed the Jackson Mining Company. The Cambria Mine, which opened in 1874, was eventually included in the property. The Cambria-Jackson Mine was consolidated with the Mather Mine, which began shipping ore in 1943. These properties were operated by the Cleveland-Cliffs Iron Company. The Mather Mine was the last underground iron mine operating in Michigan and finally closed in 1979. Cleveland-Cliffs Iron Company was formed with the merger of two major iron mining companies on Michigan's Marquette Range in 1891. The Company had a number of mines operating in the Upper Peninsula by the outbreak of World War I. By the 1940s the high grade iron ores mined underground were becoming depleted. The Company developed a process to concentrate low grade ores into iron ore pellets in the 1950s, and C. C. I. C.'s last underground iron mine closed in 1979
Mather Mine and Cambria-Jackson Mine: Caving-back operation, Cambria-Jackson Mine
Date scanned: 2002-6-20.USBM #64564; Sublevel-caving system of stoping. "Caving-back" operation 9-foot caps and pots. Ore cleaned out from right side. Ready to drill. (See USBM #64565) Cambria-Jackson Mine, The Cleveland-Cliffs Iron Company, Negaunee, Michigan - M. S. Petersen - Sept. 1946.Held in the Russell L. and Lyn Wood Mining History Archive, Arthur Lakes Library, Colorado School of Mines.Donor: United States Bureau of Mines.The first iron ore in the Lake Superior region was found on the site of the Jackson Mine, leading to discoveries of the extensive Lake Superior iron ore deposits in the Marquette Range. The Jackson pit mine was opened in 1848 by prospectors from Jackson, Michigan who formed the Jackson Mining Company. The Cambria Mine, which opened in 1874, was eventually included in the property. The Cambria-Jackson Mine was consolidated with the Mather Mine, which began shipping ore in 1943. These properties were operated by the Cleveland-Cliffs Iron Company. The Mather Mine was the last underground iron mine operating in Michigan and finally closed in 1979. Cleveland-Cliffs Iron Company was formed with the merger of two major iron mining companies on Michigan's Marquette Range in 1891. The Company had a number of mines operating in the Upper Peninsula by the outbreak of World War I. By the 1940s the high grade iron ores mined underground were becoming depleted. The Company developed a process to concentrate low grade ores into iron ore pellets in the 1950s, and C. C. I. C.'s last underground iron mine closed in 1979
sj-docx-1-pbi-10.1177_10983007231224048 – Supplemental material for The Mystery Motivator Intervention for Challenging Behavior: A Meta-Analysis
Supplemental material, sj-docx-1-pbi-10.1177_10983007231224048 for The Mystery Motivator Intervention for Challenging Behavior: A Meta-Analysis by John L. Davis, Hunter C. King, Keith C. Radley, Cambria M. Corsi, Hilary J. Jensen and William R. Jenson in Journal of Positive Behavior Interventions</p
Mather Mine and Cambria-Jackson Mine: Equipment in Mather Mine
Date scanned: 2002-6-20.USBM #64563; Safety clamp and rollers on cage for transporting rails. Mather Mine, The Cleveland-Cliffs Iron Company, Ishpeming, Michigan. (See other views USBM #64560, USBM #64561, USBM #64562) - M. S. Petersen - Sept. 1946.Held in the Russell L. and Lyn Wood Mining History Archive, Arthur Lakes Library, Colorado School of Mines.Donor: United States Bureau of Mines.The first iron ore in the Lake Superior region was found on the site of the Jackson Mine, leading to discoveries of the extensive Lake Superior iron ore deposits in the Marquette Range. The Jackson pit mine was opened in 1848 by prospectors from Jackson, Michigan who formed the Jackson Mining Company. The Cambria Mine, which opened in 1874, was eventually included in the property. The Cambria-Jackson Mine was consolidated with the Mather Mine, which began shipping ore in 1943. These properties were operated by the Cleveland-Cliffs Iron Company. The Mather Mine was the last underground iron mine operating in Michigan and finally closed in 1979. Cleveland-Cliffs Iron Company was formed with the merger of two major iron mining companies on Michigan's Marquette Range in 1891. The Company had a number of mines operating in the Upper Peninsula by the outbreak of World War I. By the 1940s the high grade iron ores mined underground were becoming depleted. The Company developed a process to concentrate low grade ores into iron ore pellets in the 1950s, and C. C. I. C.'s last underground iron mine closed in 1979
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