1,720,999 research outputs found
Bari-1, a new transposon-like family in Drosophila melanogaster with a unique heterochromatic organization
No full textdtctex-1, the Drosophila melanogaster homolog of a putative murine t-complex distorter encoding a dynein light chain, is required for production of functional sperm
No full textGenome rearrangement mediated by FB transposition: the case of ospA5 in Drosophila melanogaster
No full textCloning and characterization of a copy of Tirant transposable element in Drosophila melanogaster
No full textExalign: a new method for comparative analysis of exonintron gene structures
No full textThe evolution of genes is usually studied and reconstructed at the sequence level, that is, by comparing and aligning their genomic, transcript or protein sequences. However, including the exonintron structure of genes in the analysis can provide further and useful information, for example to draw reliable phylogenetic relationships left unsolved by traditional sequence-based evolutionary studies, or to shed further light on patterns of intron gain and loss. In spite of this, no tool especially devised for this task is currently available. In this work we present Exalign, an algorithm designed to retrieve, compare and search for the exon-intron structure of existing gene annotations, that has been implemented in a software tool freely accessible through a web interface as well as available for download. We present different applications of our method, from the reconstruction of the evolutionary history of homologous gene families to the detection of as of today unknown cases of intron loss in human and rodents, and, remarkably, two never reported intron gain events in human and mouse. The web interface for accessing Exalign is available at http:// www.pesolelab.it/exalign/ or http:// www.beacon.unimi.it/exalign/
Mutations in the glutamine syntetase I (GSI) gene produce embryo-lethal female sterility in Drosophila melanogaster.
No full textA female-sterile mutation (fs(2) PM11-19) was recovered in a screen for P-M hybrid dysgenesis induced mutations uncovered by a deletion of region 21B and was identified as an allele of the gene encoding the Drosophila glutamine synthetase I (GSI) mitochondrial isozyme. Molecular analysis has shown that fs(2)PM11-19 contains a 5 kb insert within 500 bp upstream of the transcriptional start site of the gsI gene. Mutant flies have extremely low levels of gsI transcription and GSI activity. A pre-existing deficiency (Df(2L) netPM1) with a breakpoint near the transcription start site was also found to be a female-sterile allele of gsI. All eggs laid by PM11-19 homozygous females, as well as by females heterozygous for this mutation and a deletion or any of several recessive lethal alleles of the gsI gene, fail to hatch. We conclude that an adequate level of maternally supplied GSI activity is necessary in the early stages of Drosophila embryonic development
Genetic and molecular analysis of GS genes in Drosophila melanogaster
No full textSi tratta di un poster presentato al Congresso AGI (Associazione Genetica Italiana) nel 199
Molecular mechanism for the genesis of a heterochromatic region: results of the analysis of h39 specific BAC clones in Drosophila melanogaster
No full textIntra- and interspecies variation among Bari-1 elements of the melanogaster species group
No full textThe distribution of the transposable element Bari-1 in the Drosophila melanogaster and Drosophila simulans genomes
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