96 research outputs found
Globozoospermia: the last Castle in male Infertility
No full textThe morphological and functional integrity of the acrosome in human spermatozoa is a prerequisite for attachment and binding to the ovum investments in order to allow gamete fusion. Globozoospermia is a rare condition of unknown mode of inheritance which is characterized by the complete absence of the acrosome, disorganized mid-piece, lack of zona binding and infertility. Semen analysis is normal with respect to concentration and motility. Morphological evaluation is necessary for true diagnosis of these patients. Since it is a rare disorder, consultation with andrology clinic is important in diagnostic approach. Two types of globozoospermia were described. Type I is characterized by a spherical arrangement of the chromatin and a complete lack of acrosome, therefore these spermatozoa are unable to penetrate the zona-pellucida, causing primary infertility. Type II has some acrosomal covering with a conical nucleus, which may be surrounded by large droplets of cytoplasmic material indicating secondary degenerative changes. Globozoospermia is believed to have a genetic inheritance, but the exact mode of inheritance has not yet been proven. Autosomal recessive, monogenic and polygenic inheritances were suggested to have a possible role for this unique disease. Many different investigators studied aneuploidy, Y chromosome microdeletion and structural abnormalities of chromatin, but none of them has reported definite mode of inheritance. Before IVF, these patients were completely infertile. Especially after the introduction of ICSI some authors have been reported successful pregnancies and deliveries. But the fertilization rates were lower than other patients, and in some trials pregnancies were obtained only after oocyte activation procedures
Globozoospermia - Do Y-chromosome microdeletions play a role in this rare spermatogenic disorder?
No full textOBJECTIVE: To explore the possible relationship between Y-chromosome microdeletions and a rare spermatogenic disorder, globozoospermia
Robot-assisted laparoscopic transperitoneal infrarenal lymphadenectomy in patients with locally advanced cervical cancer by single docking: Do we need a backup procedure?
No full text
Vaginal-assisted laparoscopic nerve sparing radical trachelectomy
No full textFertility-sparing surgery has gained popularity in the last three decades for the management of cervical cancer in women under 40 years of age. Radical trachelectomy is a fertility-sparing surgical technique for women who wish to retain their fertility. Vaginal-assisted laparoscopic radical trachelectomy is feasible in selected patients with early cervical cancer. The aim of this video is to present a nerve-sparing vaginal-assisted laparoscopic radical trachelectomy demonstrating pelvic anatomical structures
An unsaturated-soils approach to the bearing capacity of foundation structures
No full textUnsaturated soils are maintaining their importance for researchers and there is still much need to investigate the many engineering aspects of these soils. A new technique is proposed here to predict the variation of the bearing capacity of unsaturated soils with matric suction. The proposed method is an extension of conventional bearing-capacity theories and conceptually based on the logarithmic model of the shear strength of unsaturated soils, which only include one unknown, unsaturated parameter (the airentry value, AEV). The possibility of predicting the unsaturated bearing capacity of soils is shown by the saturated effective shear-strength parameters c\u27 and Ø\u27 and the AEV from the soil-water retention curve (SWRC). Considering the necessity of validating new methods with other researchers’ data, the proposed equation is tested using the published unsaturated experimental study by the author, in addition to some reported experimental studies on the shear strength for unsaturated soils and also a model footing loading on unsaturated sand under controlled suction conditions. The results of the study indicate that there is a good comparison between the “unsaturated bearing capacities” obtained via predicted and measured unsaturated strength parameters (ctotal , Ø) and also between the measured/ calculated bearing values of a model footing loading. Consequently, it is shown that, without needing complex unsaturated testing facilities, the proposed equation is capable of predicting the unsaturated bearing capacity for both fine-grained and sandy soils, requiring only one unsaturated parameter, which can be obtained from the SWRC or predicted using the basic soil-index properties
Failure of sentinel lymph node mapping in a patient with endometrial cancer with chronic lymphocytic leukemia: A case report
No full textWe aimed to report a detection failure of sentinel lymph node (SLN) mapping via indocyanine green in a patient with endometrial cancer with a previous history of chronic lymphocytic leukemia (CLL), which is a potential risk factor to obstruct lymphatic channels. A 64-year-old woman with a 12-year history of CLL presented to the clinic with grade 2 endometrioid carcinoma. The patient underwent laparoscopic surgical staging. Indocyanine green was used intraoperatively to locate the SLN. No lymph node or lymphatic vessels were identified during SLN mapping. At the final pathology, the morphological findings of CLL were detected in the lymph nodes without metastasis of endometrial cancer. Sentinel lymph node mapping failure due to obstruction of lymphatic channels in a patient with CLL was demonstrated in this study. This is the first report to the best of our knowledge showing SLN mapping failure in the presence of lymphoproliferative diseases
Prevalence of human papillomavirus (HPV) and HPV-16 genotyping by real-time PCR in patients with several cervical pathologies
No full textAbstractPurposeThis study was planned to evaluate the prevalence of HPV (excepting type 16) and HPV 16 by real-time PCR in colposcopy patients and to interprete the results with age, age of first sexual intercourse (FSI), parity and Pap smear results.MethodsOne hundred and two colposcopy patients (50 and 52 of the patients were classified as colposcopy positive and negative, respectively) applying to Gynecology clinic were included. HPV (excepting type 16) and HPV 16 were detected by real-time PCR using the L1 region. Real-time nested amplifications of MY09/11 products were done by GP5+/GP6+ primers and Cyanine-5 labeled HPV and HPV 16 DNA specific probe after HPV DNA extraction by phenol chloroform isoamylalcohol.ResultsHPV (excepting type 16) and HPV 16 were positive in 12% and 18% of the colposcopy positive patients respectively. HPV (excepting type 16) and HPV 16 were positive in 5.7% and 3.8% of the colposcopy negative patients, respectively.ConclusionThere was a statistically significant difference between colposcopy positive and colposcopy negative patients comparing HPV 16 with total HPV positivity (p=0.021 for type 16 and p=0.010 for total HPV) but there was not a statistically significant difference between colposcopy positive and colposcopy negative patients when we compared HPV (excepting type 16) positivity (p=0.314). In conclusion, HPV detection and typing may be helpful for cervical cancer screening and prevention
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