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    Growth and nutrient uptake of late artichoke cv. Terom

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    The aim of this research was to determine the growth rate and the nutrient uptake (N, P, K, Ca, Mg) of artichoke during the first three planting years, using the late cultivar “Terom”. Artichoke plants showed two periods of vegetative growth, in autumn and spring. In the third year, the fresh biomass production was 106.3 t ha−1 (equal to 13.6 t ha−1 of dry weight). Total nutrient uptake of artichoke plants was 272 kg ha−1 of N, 40.2 kg ha−1 of P2O5, 342.4 kg ha−1 of K2O, 314 kg ha−1 of CaO and 8.8 kg ha−1 of MgO, while the nutrient uptake ratio was 1: 0.14 : 1.25 : 1.15 : 0.03. Nutrients removal by artichoke head harvest was 32.1 kg ha−1 of N, 6.7 kg ha−1 of P2O5, 39.7 kg ha−1 of K2O, 20.6 kg ha−1 of CaO and 0.36 kg ha−1 of MgO. The highest nutrient uptake occurred in spring, during the growth of the flower stems until the harvest of the main heads

    Quali-Quantitative Determination of Chlorogenic Acid in Artichoke Heads by Means of RP-HPLC and GC-MS

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    Chlorogenic acid (trans-5-O-caffeoyl-D-quinic acid) is the quantitatively predominant hydroxycinnamate contained in artichoke (Cynara scolymus L.) tissues, and is directly involved in many of its well-documented hepatoprotective effects. The research has concerned: (a) the quali-quantitative determination of chlorogenic acid in the edible parts (inner bracts or ‘hearts’) of the main head at harvest time in 4 early artichoke cultivars from South Italy (“Violetto di Provenza”, ”Violetto di Sicilia”, “Locale di Mola”, “Thema”) and 2 late cultivars from Central Italy (“Grato 1” and “Terom”); (b) the determination of the chlorogenic acid content during the development of the main head (cv. ”Terom” and cv. “Grato 1”); (c) the evaluation of the effect of thermal treatment on the chlorogenic acid content in the edible parts of artichoke heads (cv.”Grato1”). The analysis of the methanolic extracts were carried out by means of RP-HPLC and GC/MS using chlorogenic acid methylester as internal standard. The results of cultivar comparison shows a more limited variability (ranging from 1.003 mg/g of FW in “Thema” to 2.464 mg/g of FW in “Grato 1”) than previously reported in literature, mainly due to the analytical under/overestimation of the true chlorogenic acid content without using an internal standard. The chlorogenic acid concentration decreases during the development of the main heads (i.e. for “Grato 1” a reduction of 38 % in the chlorogenic acid content has been found, going from 26 days before harvest to harvest stage). Finally, after boiling the inner bracts in water for 20 minutes, a loss of 44% in the chlorogenic acid content has been observed

    Azospirillum baldaniorum Sp245 Induces Physiological Responses to Alleviate the Adverse Effects of Drought Stress in Purple Basil

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    Azospirillum spp. are plant growth-promoting rhizobacteria (PGPR) that exert beneficial effects on plant growth and yield of agronomically important plant species. The aim of this study was to investigate the effects of a root treatment with Azospirillum baldaniorum Sp245 on hormones in xylem sap and physiological performance in purple basil (Ocimum basilicum L. cv. Red Rubin) plants grown under well-watered conditions and after removing water. Treatments with A. baldaniorum Sp245 included inoculation with viable cells (1 center dot 10(7) CFU mL(-1)) and addition of two doses of filtered culture supernatants (non-diluted 1 center dot 10(8) CFU mL(-1), and diluted 1:1). Photosynthetic activity, endogenous level of hormones in xylem sap (salicylic acid, jasmonic acid, and abscisic acid), leaf pigments, leaf water potential, water-use efficiency (WUE), and drought tolerance were determined. Fluorescence and gas exchange parameters, as well as leaf water potential, showed that the highest dose of filtered culture supernatant improved both photosynthetic performance and leaf water status during water removal, associated with an increase in total pigments. Moreover, gas exchange analysis and carbon isotope discrimination found this bacterial treatment to be the most effective in inducing an increase of intrinsic and instantaneous WUE during water stress. We hypothesize that the benefits of bacterial treatments based on A. baldaniorum Sp245 are strongly correlated with the synthesis of phytohormones and the induction of plant-stress tolerance in purple basil
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