5,491 research outputs found
Application of Gloss methodology to 3NO cod
No full textThis document reports the application of the G(loss) approach (Cook, 1998) to cod in 3NO. This method calculates the replacement line associated with the lowest observed spawning stock biomass, B(loss), and the probability that a given fishing mortality rate exceeds this replacement line. If the fishing mortality rate exceeds G(loss) then the stock will tend to decline below the lowest observed SSB
The Systematic Integrated Learning Adviser program at James Cook University
No full textThe Systematic Integrated Learning Adviser (SILA) program currently in place at James Cook University is one of this institution's responses to the numerous drivers pressing universities to examine the ways in which they provide English language development to students. Two key factors underpinned the original rationale for this program. First, the widening participation agenda flowing from the recommendations of the Bradley et al. (2009) review of higher education continues to have a tangible impact on the both the diversity and preparedness for academic study of first year students entering the institution. Second, a growing body of research indicates that the 'traditional' methods of providing academic literacies support to our students are often ineffective and may be seen as unsustainable in the current funding environment (Dunworth, 2013). At the same time, the clear signals provided by TEQSA concerning the central importance of students' English language proficiency (TEQSA, 2012) have provided impetus for internal policy makers to connect the concerns and experiences of ‘on the ground’ teaching staff (including learning advisers) to institutional actions.
This presentation details the ongoing progress of the SILA program with particular reference to the good practice principles described in the document, Good practice in developing an institutional strategy for post-entry student English language proficiency (Dunworth, Drury, Kralik, Moore & Mulligan, 2013). The presentation considers the evolving 'place' of post entry language assessments (PELA) and draws on the preliminary results of the SILA evaluation research project to explore the challenges and success experienced to date
Wellesly Sh. W. to Mr. James Meredith (2 October 1962)
No full textSigned by Wellesly Sh. W.https://egrove.olemiss.edu/mercorr_pro/1531/thumbnail.jp
Effets d'un traitement chronique par la morphine sur le protéome des cellules de neuroblastome humain SH-SY5Y
No full textAfin de mieux comprendre les adaptations cellulaires induites suite à une prise chronique de morphine, une stratégie de type protéomique différentielle combinant les techniques d'électrophorèse et de spectrométrie de masse a été entreprise dans des cellules de neuroblastome humain sh-sy5y surexprimant le récepteur mop. l'étude globale des modifications induites par la morphine sur le protéome total a permis d'identifier environ 50 protéines impliquées dans des processus tels que l'organisation du cytosquelette, le trafic vésiculaire, la dégradation des protéines et la signalisation cellulaire. de plus, nous avons montré qu'un traitement chronique à la morphine entraînait la dégradation par le protéasome de sous-unités de protéines g; la dégradation deg étant étroitement corrélée à la sensibilisation de l'adénylyl cyclase, un phénomène impliqué dans la dépendance à la morphine. nos travaux suggèrent ainsi que le protéasome est impliqué dans les effets à long terme de la morphine
Scottish Parliament Committee on Culture, Tourism, Europe and External Affairs; Inquiry on the Negotiations of the Future Relationship between the European Union and the UK Government : Submission for Professor Michael Heath, Dr Robin Cook and Professor Paul Fernandes
No full textWritten submission (CTEEA/S5/20/FR/012) to the Scottish Parliament Inquiry on the Negotiations of the Future Relationship between the European Union and the UK Government, focusing specifically on fisheries and zonal attachment as a principle for dividing fishing opportunities
Fish population dynamics, monitoring and management sustainable fisheries in the eternal ocean By I. Aoki, T. Yamakawa & A. Takasuka Springer, Japan, 2018
No full textIn the words on the cover of this book “it offers readers a broad understanding of the current methods and theory for sustainable exploitation of fisheries resources, and introduces recent findings and technological developments.” This is a fair description of its scope which is covered in just under 250 pages. Inevitably for a concise volume with such a breadth of material to include, some topics are dealt with in a very cursory manner and there are notable omissions, but it makes a good read, especially for those interested in learning about how fisheries are managed in the seas around Japan
Inclusion of discards in stock assessment models
No full textA large portion of the catch in many stocks may comprise discards which need to be accounted for in assessments in order to avoid bias in estimates of fishing mortality, stock biomass and reference points. In age structured assessment models, discards are sometimes treated as a separate fleet or are added to the landings before fitting so that information about discard behavior and sampling error is lost. In this paper an assessment model is developed to describe the discard process with size as a covariate while retaining age structured population dynamics. Discard size selection, high grading and bulk dumping of fish at sea are modelled so that the temporal dynamics of the process can be quantified within the assessment. The model is used to show that discarding practices have changed over time in a range of Northeast Atlantic demersal fish. In some stocks there is a substantial increase in high grading and evidence for bulk discarding which can be related to regulatory measures. The model offers a means of identifying transient effects in the discard process that should be removed from both short-term forecasts and equilibrium reference point calculation
SMA-SH: Modified styrene maleic acid copolymer for functionalization of lipid nanodiscs
No full textChallenges in purification and subsequent functionalization of membrane proteins often complicate their biochemical and biophysical characterization. Purification of membrane proteins generally involves replacing the lipids surrounding the protein with detergent molecules, which can affect protein structure and function. Recently, it was shown that styrene–maleic acid copolymers (SMA) can dissolve integral membrane proteins from biological membranes into nanosized discs. Within these nanoparticles, proteins are embedded in a patch of their native lipid bilayer that is stabilized in solution by the amphipathic polymer that wraps the disc like a bracelet. This approach for detergent-free purification of membrane proteins has the potential to greatly simplify purification but does not facilitate conjugation of functional compounds to the membrane proteins. Often, such functionalization involves laborious preparation of protein variants and optimization of labeling procedures to ensure only minimal perturbation of the protein. Here, we present a strategy that circumvents several of these complications through modifying SMA by grafting the polymer with cysteamine. The reaction results in SMA that has solvent-exposed sulfhydrils (SMA-SH) and allows tuning of the coverage with SH groups. Size exclusion chromatography, dynamic light scattering, and transmission electron microscopy demonstrate that SMA-SH dissolves lipid bilayer membranes into lipid nanodiscs, just like SMA. In addition, we demonstrate that, just like SMA, SMA-SH solubilizes proteoliposomes into protein-loaded nanodiscs. We covalently modify SMA-SH-lipid nanodiscs using thiol-reactive derivatives of Alexa Fluor 488 and biotin. Thus, SMA-SH promises to simultaneously tackle challenges in purification and functionalization of membrane proteins.BN/Marie-Eve Aubin-Tam LabBN/Andreas Engel La
Omega-3 fatty acid eicospentaenoic acid attenuates MPP+-induced neurodegeneration in fully differentiated human SH- SY5Y and primary mesencephalic cells
No full textEicosapentaenoic acid ( EPA), a neuroactive omega-3 fatty acid, has been demonstrated to exert neuroprotective effects in experimental models of Parkinson's disease ( PD), but the cellular mechanisms of protection are unknown. Here, we studied the effects of EPA in fully differentiated human SH-SY5Y cells and primary mesencephalic neurons treated with MPP+. In both in-vitro models of PD, EPA attenuated an MPP+-induced reduction in cell viability. EPA also prevented the presence of electron-dense cytoplasmic inclusions in SH-SY5Y cells. Then, possible mechanisms of the neuroprotection were studied. In primary neurons, EPA attenuated an MPP+-induced increase in Tyrosine-related kinase B (TrkB) receptors. In SH-SY5Y cells, EPA down-regulated reactive oxygen species and nitric oxide. This antioxidant effect of EPA may have been mediated by its inhibition of neuronal NADPH oxidase and cyclo-oxygenase-2 ( COX-2), as MPP+ increased the expression of these enzymes. Furthermore, EPA prevented an increase in cytosolic phospholipase A2 ( cPLA2), an enzyme linked with COX-2 in the potentially pro-inflammatory arachidonic acid cascade. Lastly, EPA attenuated an increase in the bax:bcl-2 ratio, and cytochrome c release. However, EPA did not prevent mitochondrial enlargement or a decrease in mitochondrial membrane potential. This study demonstrated cellular mechanisms by which EPA provided neuroprotective effects in experimental P
An assessment of scallops, Pecten maximus Linnaeus, 1758), in Scotland west of Kintyre
No full textThis paper describes an assessment of a fishery for Pecten maximus using virtual population analysis, a method much used in ICES fish stock assessments. Despite obvious differences between bivalves and fish in both biology and the natures of the fisheries, the method performed adequately on data aggregated over large areas. Long term trends in yield, stock biomass and recruitment calculated from annual VPA are described. Total stock biomass and recruitment are clearly related, high biomass following years of good recruitment. Recruitment appears more important than fishing mortality in determining stock biomass. It is tentatively suggested that recruitment a year ahead might be predicted; if so, prospects of forecasting catches would be good. The yield per recruit curve is flat-topped, indicating that the present realized values are near the maximum likely to be obtained. It is concluded that there is no special reason for concern about the present level of exploitation of the stock. -Author
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