170,006 research outputs found

    ELECTROANALYTICAL DETERMINATION OF SOME PHENOLIC ACIDS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AT GOLD ELECTRODES.

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    The electrochemical and amperometric behavior of a gold electrode was investigated towards the oxidation of several common phenolic acids in neutral phosphate solutions. Au electrodes show an appreciable stability and reproducibility of the amperometric signals by using a constant applied potential of 1.0 V vs. Ag/AgCl. Separations of selected phenolic acids using a reverse phase C-18 analytical column with a mobile phase containing 10 mM NaH2 PO4 Plus 10 mM Na2HPO4 (pH 7) and methanol as organic modifier, are achieved isocratically in less than 30 min. The detection limits at the level of nmol/L and linear ranges of four-five orders of magnitude are generally achieved. The proposed chromatographic strategy coupled with the electrochemical detection at the Au electrode was successful tested for the quantitative determination of phenolic acids in beer, red wine and brandy with good sensitivity and recover

    Comparative Metagenomics of Eight Geographically Remote Terrestrial Hot Springs.

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    Hot springs are natural habitats for thermophilic Archaea and Bacteria. In this paper, we present the metagenomic analysis of eight globally distributed terrestrial hot springs from China, Iceland, Italy, Russia, and the USA with a temperature range between 61 and 92 (∘)C and pH between 1.8 and 7. A comparison of the biodiversity and community composition generally showed a decrease in biodiversity with increasing temperature and decreasing pH. Another important factor shaping microbial diversity of the studied sites was the abundance of organic substrates. Several species of the Crenarchaeal order Thermoprotei were detected, whereas no single bacterial species was found in all samples, suggesting a better adaptation of certain archaeal species to different thermophilic environments. Two hot springs show high abundance of Acidithiobacillus, supporting the idea of a true thermophilic Acidithiobacillus species that can thrive in hyperthermophilic environments. Depending on the sample, up to 58 % of sequencing reads could not be assigned to a known phylum, reinforcing the fact that a large number of microorganisms in nature, including those thriving in hot environments remain to be isolated and characterized
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