1,721,070 research outputs found
VIII Conferenza Internazionale della Comunicazione Sociale GIOVANI FUTURO INNOVAZIONE SOCIALE
No full textEffect of semen preparation on casa motility results in cryopreserved bull spermatozoa
No full textComputer-assisted sperm analyzers (CASA) have become the standard tool for evaluating sperm motility and kinetic patterns because they provide objective data for thousands of sperm tracks. However, these devices are not ready-to-use and standardization of analytical practices is a fundamental requirement. In this study, we evaluated the effects of some settings, such as frame rate and frames per field, chamber and time of analysis, and samples preparations, including thawing temperature, sperm sample concentration, and media used for dilution, on the kinetic results of bovine frozen-thawed semen using a CASA. In Experiment 1, the frame rate (30-60 frame/s) significantly affected motility parameters, whereas the number of frames per field (30 or 45) did not seem to affect sperm kinetics. In Experiment 2, the thawing protocol affects sperm motility and kinetic parameters. Sperm sample concentration significantly limited the opportunity to perform the analysis and the kinetic results. A concentration of 100 and 50 x 10(6) sperm/mL limited the device's ability to perform the analysis or gave wrong results, whereas 5, 10, 20, and 30 x 10(6) sperm/mL concentrations allowed the analysis to be performed, but with different results (Experiment 3). The medium used for the dilution of the sample, which is fundamental for a correct sperm head detection, affects sperm motility results (Experiment 4). In this study, Makler and Leja chambers were used to perform the semen analysis with CASA devices. The chamber used significantly affected motility results (Experiment 5). The time between chamber loading and analysis affected sperm velocities, regardless of chamber used. Based on results recorded in this study, we propose that the CASA evaluation of motility of bovine frozen-thawed semen using Hamilton-Thorne IVOS 12.3 should be performed using a frame rate of 60 frame/s and 30 frames per field. Semen should be diluted at least at 20 x 10(6) sperm/mL using PBS. Furthermore, it is necessary to consider the type of chamber used and perform the analysis within 1 or 2 min, regardless of the chamber used.[...
Effect of sperm concentration on characteristics of frozen-thawed semen in donkeys
No full textIn this study, the effect of donkey sperm concentration in the straw during cryopreservationon the quality of thawed semen was evaluated. Samples from seven adult Martina Francajackasses were collected three times using a Missouri artificial vagina. After estimation ofvolume and concentration, raw semen was evaluated for motility using a computer-assistedsperm analyzer (CASA); viability and acrosome integrity were also determined. Fresh semenwas then centrifuged and re-suspended at five different concentrations (100, 250, 500,750, and 1000×106 sperm/ml) with a commercial extender, packaged in 0.5 ml straws, andfrozen. After thawing, motility parameters, viability, and acrosome integrity were analyzed.The analysis of the data showed similar parameters of fresh semen compared with those ofcentrifuged and cooled samples. The sperm concentration in the straw affected the semenparameters analyzed after thawing, as suggested by evidence that when the concentrationincreased, the quality of the post-thawed semen decreased. Furthermore, the differencesin total and progressive motility among samples at different concentrations are due to theimmobilization of spermatozoa, as suggested by the finding that the percentage of staticspermatozoa increased when the concentration increased. The reason for the impairmentof semen quality when the sperm concentration increased was discussed. A great variabilityin cryo-resistance was found between jackasses but not within the same male, suggestingthe presence of donkey males with semen that has acceptable and unacceptable freezingqualities.[...
Venous blood gas parameters in healthy Mediterranean buffalo calves in the first 72 hours of life
No full textEffectiveness of a modified administration protocol for the medical treatment of canine pyometra
No full textPyometra is one of the most common diseases in intact bitches. The aim of this study was to evaluate the effectiveness of a modified aglepristone protocol for the medical treatment of pyometra in the bitch. Of these, 73 bitches affected by pyometra of different breeds and age (2–14 years old) were enrolled. They were randomly assigned to a control group (CTG - 26 bitches) treated with classical protocol (aglepristone at 0, 1 and 6 days - day 0 = day of the diagnosis) and a modified treated group (MTG - 47 bitches) treated with a different administration protocol (aglepristone at 0, 2, 5 and 8 days). The classical protocol with the anti-progestagen aglepristone was effective in 88.5 % (23/26) of CTG bitches while the modified protocol was effective in all (47/47) of MTG bitches. One of the 23 CTG bitches received a further administration on day 14, which resolved the pyometra, while in the three cases of CTG bitches, in which the treatment was ineffective, an ovariohysterectomy was carried out. The modified protocol showed a success rate of 100 %, compared with the classical protocol proposed in the literature, and no recurrence of the disease was recorded in the 24 months follow up. After treatment, the oestrus onset was earlier than expected (interoestrus of 128 ± 32 days). In this study, the modified treatment protocol showed high efficacy and lack of recurrence within 24 months, suggesting a complete recovery of reproductive function in the bitch, with a normal fertility
Studio preliminare su alcune caratteristiche seminali e morfometriche testicolari dello stallone asinino di Martina Franca
No full textBreeding soundness evaluation of equine stallions is a routine component of stud farm medicine. Guidelines for assessingsatisfactory breeding potential have been well described in the horse. Instead, only few studies have been performed on thebreeding parameters in the jackass. The aim of this study is to evaluate some seminal and testicular morphometric characteristicsof Martina Franca jackass.[...
Successful use of a gonadotropin-releasing hormone (GnRH) analog for the treatment of tertiary hypogonadism (GnRH deficiency) in a 5-year-old Belgian Blue bull
No full textA bull was referred for a progressive oligoasthenotheratozoospermia that resulted in a unsuitable seminal quality for the cryopreservation. Breeding soundness evaluation results suggested gonadal dysfunction. Because of the lack of normal ranges for these hormones in the bull, in this study, the hypogonadism and the site of the dysfunction (hypothalamus) were diagnosed by the gonadotropin-releasing hormone (GnRH) stimulation test. The evaluation of pituitary and testicular responsiveness by a GnRH stimulating test revealed a responsiveness of the pituitary and testis, thus a secondary hypogonadism (hypothalamic hypogonadism) was postulated and a therapeutic approach based on the subcutaneous administration of GnRH analog was attempted. An increase in semen volume, concentration and sperm characteristics were detected 9 weeks after the start of the treatment, corroborating the hypothalamic origin of the disease and the useful of the GnRH therapy.[...
Studio preliminare sulla longevità del materiale seminale dell’asino di Martina Franca refrigerato a 5°C con diversi diluitori
No full textLa composizione in acidi grassi della membrana spermatica influenza la qualità del materiale seminale dopo crioconservazione nello stallone
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