1,720,964 research outputs found
La PCR come mezzo di identificazione dei fattori di virulenza di Bacillus anthracis. Dati preliminari sull’analisi del ceppo vaccinale “CARBOSAP” utilizzato nelle campagne vaccinali contro il carbonchio ematico ovino e bovino in Italia
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Test di western blotting per differenziare le infezioni da brucella spp. e Yersinia enterocolitica O:9 negli ovini
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Indagine sulla diffusione della leishmaniosi nel cane e nell'uomo sul promontorio del Gargano
No full textDevelopment of a Western blotting assay to discriminate Brucella spp. and Yersinia enterocolitica O:9 infection in sheep
No full textRicerca di Mycoplasma agalactiae nel latte di pecora mediante la Polymerase Chain Reaction
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Development of a Western Blotting assay to discriminate Brucella spp. and Yersinia enterocolitica O:9 infections in sheep
No full textOuter membrane proteins (OMPs) of Rev-1 strain of Brucella melitensis were used in a Western blotting assay for the serological diagnosis of brucellosis in ovine sera. Fifty-four sheep sera were tested and divided into the following groups: Group A) n. 9 samples from one sheep that had been experimentally infected with Y. enterocolitica O:9; Group B) n. 10 samples collected from sheep infected with Brucella melitensis and 1 sample from a sheep vaccinated with the Rev 1 strain; Group C) n. 10 samples collected in "officially brucellosis-free" herds; Group D) n. 12 samples classified as "suspicious"; Group E) n. 12 samples classified as "positive". Antibodies were detected by routine tests performed for the diagnosis of brucellosis in serum samples of the sheep infected with Y. enterocolitica O:9 after the 2nd week post infection. In the WB assay, sera of group B recognised a 17 kDa protein, whereas sera of groups A, and D and 9 out of 12 of group E exhibited no reactivity to this protein. The results obtained encourage the use of the WB assay as a confirmatory test for the diagnosis of brucellosis
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