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Oligonucleotidi sintetici per l'identificazione dei virus Herpes simplex e la tipizzazione dei virus Herpes simplex di tipo 1 e di tipo 2 mediante la tecnica di polymerase chain reaction (PCR) ed ibridazione molecolare, loro uso e kits diagnostici che li contengono.
No full textLa presente invenzione si riferisce ad oligonucleotidi sintetici che permettono di identificare i virus Herpes simplex e di tipizzare i virus Herpes simplex di tipo 1 e di tipo 2 mediante la tecnica di polymerase chain reaction (PCR) ed ibridazione molecolare. L’invenzione si riferisce inoltre al loro uso per le determinazioni e tipizzazioni suddette e a kits diagnostici che li contengono
CV/BKV and SV40 viral load in lymphoid tissues of young immunocompetent children from an area of North-East Italy
No full textPolyomavirus infection occurring during childhood
is followed by a lifelong latency in immunocompetent
subjects. The major site of
polyomavirus persistence are the uroepithelial
cells which leads to oral transmission. It has
recently been hypothesized that tonsils could be a
possible reservoir. The role of tonsil, adenoid,
and peripheral blood mononuclear cells (PBMCs)
as possible sites of JCV, BKV, and SV40 latency
in young healthy children was assessed. Two
hundred fifteen fresh specimens, including 57
tonsil, 80 adenoid, and 78 PBMC samples from 80
immunocompetent children (mean age 4.8 years)
were analyzed to determine the viral load by
quantitative real-time PCR. The human herpes
virus 6 (HHV-6) was tested as a lymphotropic
reference virus. Polyomavirus was detected in 5/
80 (6.2%) children while HHV-6 infection affected
27/80 children (33.7%) (P<0.001). SV40 was
detected in one adenoid sample, while footprints
of BKV were found in one adenoid and three
tonsil samples. JCV was never found in all
samples. Polyomavirus sequences were not
detected in the 78 blood samples. One adenoid
and two tonsils from three children (1.4%) were
positive for both polyomavirus and HHV-6.
Infections were characterized by low replication
rates ranging typically from 110e2/5.510e4 to
6.810e3/8.510e4 viral copies/number of cells.
In conclusion, tonsils and adenoids of children
could effectively harbor BKV and SV40, although
only very few cells proved to be infected. Nevertheless,
the low prevalence of polyomavirus, in
comparison with the lymphotropic HHV-6, suggests
that these tissues are unlikely to be the
preferred site of polyomavirus latency, at least
in younger children
A quantitative procedure for monitoring expression of human immuno deficiency virus type 1.
No full text
Molecular diagnosis of opportunistic pericardial infection in a patient treated with adalimumab: the role of next-generation sequencing
No full textBiological immune-modulator drugs, especially inhibitors of tumor necrosis factor-α, are frequently encountered in modern clinical practice and opportunistic infections are therefore a common concern. Infective pericarditis has been described as a complication of these treatments with possible life-threatening consequences. In similar cases cultures may isolate multiple opportunistic bacteria from the pericardial fluid without specific identification of the responsible germ, representing a problem for targeted antibiotic therapy. We present a case of acute pericarditis evolving in pericardial constriction and cardiac tamponade in a patient treated with adalimumab for psoriatic arthritis overlapping with recurrent polychondritis. Next-generation sequencing allowed the identification of a common oral pathogen as the aetiological agent confirming its role in the identification of species that can be overlooked by common microbiological techniques
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