1,721,063 research outputs found
Caratterizzazione genomica di uno stipite di Coronavirus associato ad enterite nelle quaglie di allevamento
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Validation of a seminested PCR approach for rapid detection of Salmonella enterica subsp. enterica serovar Gallinarum
No full textSalmonella enterica subsp. enterica serovar Gallinarum (S. Gallinarum) is the causative agent of fowl typhoid,
one of the major causes of mortality and morbidity on poultry farms. Even though it has been substantially
eradicated in many developed countries, the disease still remains endemic in Central and South America, in
Africa and in the Mediterranean countries of Europe. This leads to the routine screening of flocks, mainly by
cultivation and serological techniques, which are expensive, as well as time and labour-consuming. Here we
describe a simple and specific PCR-based method for detecting S. Gallinarum. It relies on two seminested PCRs
which use four pairs of primers designed on the basis of two genomic regions which appear to be exclusive to
the pathogen. Furthermore, an internal positive control was devised in order to avoid any false negative
results. We performed sensitivity and specificity tests, and our findings showed the cogency of the system and
its potential effectiveness even for routine uses
Poultry as Salmonella infection source for workers in laying hens farm and egg packaging plants
No full textAspetti epidemiologici delle infezioni da Campylobacter jejuni negli allevamenti di pollame
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