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Metodo per la preparazione di Interleuchina 6
No full textMethod for the preparation of interleukin 6.
A process for the preparation of interleukin 6 from transformed bacterial strains which comprises:
a) the culture of a bacterial host transformed with an exspression vector coding for interleukin-6;
b) bacterial lysis, isolation by centrifugation of inclusion bodies containing interleukin-6 and their denaturation with guanidine hydrochloride;
c) dialysis of the denaturated proteins in the presence of a redox system, preferably reduced and oxidized glutathione;
d) anion-exchange chromatography of the dialysate on DEAE-Sepharose, eluting with a pH adjusted saline water-glycerol solution gradient, selecting and collecting the fractions containing interleukin-6 at the desired purity
The human Ą1-antitrypsin gene is transcribed from two different promoters in macrophages and hepatocytes.
No full textIdentification of sequences responsible for acute-phase induction of human C-reactive protein
No full textHuman C-Reactive protein (CRP) is inducible in liver cells during acute inflammation. Around 90 bp from the 5' flanking region of the human CRP gene contain, as shown here, information to induce the expression of a linked bacterial CAT gene specifically in human hepatoma (Hep3B) cells. The promoter is induced rapidly, faithfully and at high efficiency when transfected cells are exposed to conditioned medium from lipopolysaccharide stimulated peripheral monocytes. The sequences required for inducibility are located immediately upstream to the TATA element. A DNA segment from base -121 to -50 is capable of inducing transcription from the heterologous SV40 early promoter. Induction of CRP expression is probably exerted via the binding of at least one positive trans-acting factor
A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.
No full textThe emerging role of cancer cell plasticity and cell-cycle quiescence in immune escape
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A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.
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