86 research outputs found
Theranostic designed near-Infrared fluorescent poly (lactic-co-glycolic acid) nanoparticles and preliminary studies with functionalized VEGF-nanoparticles
No full textPoly-lactic-co-glycolic acid nanoparticles (PLGA-NPs) were approved by the Food and Drug Administration (FDA) for drug delivery in cancer. The enhanced permeability and retention (EPR) effect drives their accumulation minimizing the side effects of chemotherapeutics. Our aim was to develop a new theranostic tool for cancer diagnosis and therapy based on PLGA-NPs and to evaluate the added value of vascular endothelial growth factor (VEGF) for enhanced tumor targeting. In vitro and in vivo properties of PLGA-NPs were tested and compared with VEGF-PLGA-NPs. Dynamic light scattering (DLS) was performed to evaluate the particle size, polydispersity index (PDI), and zeta potential of both preparations. Spectroscopy was used to confirm the absorption spectra in the near-infrared (NIR). In vivo, in BALB/c mice bearing a syngeneic tumor in the right thigh, intravenously injected PLGA-NPs showed a high target-to-muscle ratio (4.2 T/M at 24 h post-injection) that increased over time, with a maximum uptake at 72 h and a retention of the NPs up to 240 h. VEGF-PLGA-NPs accumulated in tumors 1.75 times more than PLGA-NPs with a tumor-to-muscle ratio of 7.90 ± 1.61 (versus 4.49 ± 0.54 of PLGA-NPs). Our study highlights the tumor-targeting potential of PLGA-NPs for diagnostic and therapeutic applications. Such NPs can be conjugated with proteins such as VEGF to increase accumulation in tumor lesions
Ambiente, diritti e cittadinanza
No full textAfter reflecting on the environment and its multiple meanings, the author goes on to examine the rights of the environment and to the environment as a prerequisite for the construction of a good quality of life. The environmental issue from the point of view of citizenship implies rights and obligations and involves perceptions and representations of the world on the basis of responsibility, respect, sharing and collaboration. Hence, the pedagogical role of an education for the environment and of the environment
Weak solutions of a conservation law with memory
No full textIn this paper, the author obtains the existence and uniqueness of weak solutions of the Cauchy problem for a quasilinear equation of conservation type with memory ut +f(u)x +a∗ψ(u) = 0, u(0,x) = u0(x), in one space variable. The main additional hypothesesareψ(0)=0,ψ′ >0and(−1)na(n) ≥0forn=0,1,2
Gongsun Long and the Gongsun Longzi: authorship and textual variation in a multilayered text
No full textIl presente lavoro si pone come obiettivo il gettare nuova luce sulla struttura e il contenuto del Gongsun Longzi, concentrandosi in particolare sui cosiddetti “capitoli originali”, ovvero quelli considerati i più fedeli ad una ipotetica formulazione originaria – scritta e/od orale - degli argomenti discussi, il Baima Lun ed il Zhiwu Lun. Dopo aver preso il considerazione la figura pseudo-storica dell’autore putativo, Gongsun Long, viene fornita un’analisi della struttura complessiva del testo, incluso anche un accurato studio delle variati testuali esistenti tra le due versioni più antiche del testo ricevuto (le edizioni dello Shuofu e del Daozang). Infine, un’esaustiva trattazione dei contenuti filosofici del Baima Lun e del Zhiwu Lun, corredata da una traduzione commentata, chiude il lavoroThe present work aims at shading new light on the structure and content of the Gongsun Longzi, focusing in particular on the so-called “original chapters”, those who are considered more truthful to an hypothetical original formulation – written and/or oral – of the topics discussed, the Baima Lun and the Zhiwu Lun. After taking into consideration the pseudo-historical figure of the putative author, the persuader Gongsun Long, an analysis of the overall structure of the text is provided, comprehending an accurate study of textual variants existing between the two most ancients versions available of the received text (the Shuofu and the Daozang edition). Finally, an exhaustive treatment of the philosophical contents of the Baima Lun and Zhiwu Lun, accompanied with a commented translation, concludes the work
"Against Palladio: Reading The Stones of Venice in the train between Vicenza and Venice"
No full textEssay on Ruskin's influence on American author
Inhibition of Mek 1/2 kinase activity and stimulation of melanogenesis by 5,7-dimethoxycoumarin treatment of melanoma cells
No full textIn this study, the processes of differentiation and melanogenesis induced by 5.7-dimethoxycoumarin in murine (B16) and human (A375) melanoma cells were investigated. Taking into account the previously demonstrated anti-proliferative and differentiation activities of this compound, we examined Ras/Raf/Mek/Erk mitogen-activated protein kinase activity following treatment; inhibition of Mek 1/2 kinase activity and Subsequent reduction in Erk 1/2 activation were detected in both cell types. We observed melanogenesis induction associated to an increase in cAMP-response element-binding protein (CREB) and microphthalmia-associated transcription factor (Mitf) expression, both involved in its regulation. Mitf is fundamental for development, survival and differentiation of melanocyte and melanoma, since it regulates transcription of genes encoding for proteins involved in cell cycle progression or in melanogenesis, such as the enzyme tyrosinase. A significant increase of tyrosinase activity was revealed following treatment in B16 but not in A375 cells. although a strong synthesis of melanin was induced by 5,7-dimethoxycoumarin in both cell lines. The treatment induced protoporphyrine IX accumulation involved in melanogenesis since it promotes stability of cAMP. Finally, the Mek 1/2 inhibitor U0126 significantly potentiated growth inhibition of B16 cells triggered by 5,7-dimethoxycoumarin, suggesting that down-regulation of Raf/Mek/Erk pathway sensitizes melanoma cells to 5,7-dimethoxycoumarin treatment
Mycobacterial 19-kDa lipoprotein mediates Mycobacterium tuberculosis-induced apoptosis in monocytes/macrophages at early stages of infection
No full textInterazioni delle cellule del sistema immunitario naturale con il micobatterio della tubercolosi: meccanismi di controllo e di evasione nella risposta anti-tumorale
No full textDottorato di ricerca in immunologia. 11. ciclo. Coordinatore Gino Doria. Relatore Vittorio ColizziConsiglio Nazionale delle Ricerche - Biblioteca Centrale - P.le Aldo Moro, 7, Rome; Biblioteca Nazionale Centrale - P.za Cavalleggeri, 1, Florence / CNR - Consiglio Nazionale delle RichercheSIGLEITItal
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