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In vitro steroid secretion by intertubular and tubular compartments of Reptiles Podarcis s. sicula) testis
No full textIn vitro feed-back of sex hormones on hypothalamus-pituitary axis in the lizard Podarcis s. sicula Raf
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PLASMA SEX STEROID BINDING-PROTEINS (SSBP) IN THE MALE LIZARD, PODARCIS-SICULA-SICULA, DURING THE REPRODUCTIVE-CYCLE
No full textIn male Podarcis s. sicula plasma, a sex steroid binding protein [SSBP(s)] binds testosterone (T) and estradiol-17β (E2) with moderate affinity (Kd = 0.23 ± 0.08 × 10-8 for 3H-E2, and 0.24 ± 0.07 × 10-8 for 3H-T) and high capacity. The SSBP binding affinity is unchanged throughout the sexual cycle, although its capacity is higher in nonreproductive males (winter and postreproductive period). This change may be related to changes in plasma T and E2 levels, and is likely to be involved in mechanisms whereby free steroid is delivered to target organs. SSBP, under isoelectrofocusing, is distributed between pH 5.5-6.5 and pH 7.1-7.5. The concentration of these two forms varies during the annual cycle. © 1992
Progesterone and 17ß-estradiol binding molecules in the oviduct of the lizard, Podarcis s. sicula, during the sexual cycle
No full textSEX-HORMONES DURING THE ANNUAL CYCLE IN 2 LIZARD SPECIES - PODARCIS S SICULA (OVIPAROUS) AND CHALCIDES-OCELLATUS (VIVIPAROUS)
No full textOVIDUCT 17-BETA-ESTRADIOL RECEPTOR IN THE FEMALE LIZARD, PODARCIS-SICULA-SICULA, DURING THE SEXUAL CYCLE - RELATION TO PLASMA 17-BETA-ESTRADIOL CONCENTRATION AND ITS BINDING-PROTEINS
No full textIn the oviparous lizard, Podarcis s. sicula, 17beta-estradiol binding molecules were characterized in the oviduct and plasma. In addition, their concentration was evaluated throughout the annual cycle.In the oviduct, a 17beta-estradiol receptor (ER) is present. It shows high affinity for ligand (4.9-7.0 x 10(-10) M). It is decreased by ovariectomy, and induced by 17beta-estradiol treatment. 17beta-estradiol causes also a ER shift from the cytosol into the nuclei. On sucrose gradient ER behaviour is consistent with the properties of ER obtained from other 17beta-estradiol target organs. At isoelectrofocusing the labeled 17beta-estradiol binding molecules fall into three pH ranges: 5.2-5.6, 7.0-7.7, 8,0-8.7.Nuclear filled ER significantly increases as oviduct grows. This supports an ER role in oviduct stimulation by 17beta-estradiol.In the plasma, 17beta-estradiol binding molecules show many physico-chemical and behavioural properties of steroid-binding proteins (SBPs)
Rilascio in vitro di ormoni sessuali dal testicolo della lucertola Podarcis s. sicula Raf
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