158 research outputs found

    The mitochondrial genome sequence of Abies alba Mill. reveals a high structural and combinatorial variation

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    Abstract Background Plant mitogenomes vary widely in size and genomic architecture. Although hundreds of plant mitogenomes of angiosperm species have already been sequence-characterized, only a few mitogenomes are available from gymnosperms. Silver fir (Abies alba) is an economically important gymnosperm species that is widely distributed in Europe and occupies a large range of environmental conditions. Reference sequences of the nuclear and chloroplast genome of A. alba are available, however, the mitogenome has not yet been assembled and studied. Results Here, we used paired-end Illumina short reads generated from a single haploid megagametophyte in combination with PacBio long reads from high molecular weight DNA of needles to assemble the first mitogenome sequence of A. alba. Assembly and scaffolding resulted in 11 mitogenome scaffolds, with the largest scaffold being 0.25 Mbp long. Two of the scaffolds displayed a potential circular structure supported by PCR. The total size of the A. alba mitogenome was estimated at 1.43 Mbp, similar to the size (1.33 Mbp) of a draft assembly of the Abies firma mitogenome. In total, 53 distinct genes of known function were annotated in the A. alba mitogenome, comprising 41 protein-coding genes, nine tRNA, and three rRNA genes. The proportion of highly repetitive elements (REs) was 0.168. The mitogenome seems to have a complex and dynamic structure featured by high combinatorial variation, which was specifically confirmed by PCR for the contig with the highest mapping coverage. Comparative analysis of all sequenced mitogenomes of gymnosperms revealed a moderate, but significant positive correlation between mitogenome size and proportion of REs. Conclusions The A. alba mitogenome provides a basis for new comparative studies and will allow to answer important structural, phylogenetic and other evolutionary questions. Future long-read sequencing with higher coverage of the A. alba mitogenome will be the key to further resolve its physical structure. The observed positive correlation between mitogenome size and proportion of REs will be further validated once available mitogenomes of gymnosperms would become more numerous. To test whether a higher proportion of REs in a mitogenome leads to an increased recombination and higher structural complexity and variability is a prospective avenue for future research

    Resilient forests for the future

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    Forest ecosystems are of global importance, ecologically, economically and culturally. However, despite their fundamental role in mitigating the worst effects of climate change, to date there have been surprisingly few resources devoted to defining, conserving and planning resilient forests for the future. Progress in this field of research, which requires international and interdisciplinary cooperation, collaboration and communication, was presented and discussed at the second biannual conference of the European Research Group, Evoltree (https://www.evoltree.eu). Over four days more than 140 scientists met to share developments and to discuss forest ecology, genetics, genomics and evolution with a focus on realising “Resilient Forests for the Future”. From examining evolutionary dynamics and using the past to understand future responses, to evaluating breeding approaches and the sustainable use of forest genetic resources, the conference addressed critical themes with relevance to this topic. The role of genomics in conservation, investigation of biotic interactions and identifying climate resilient forests were also explored. Finally, innovative methods and approaches which promise to increase the scale and speed with which forest evolutionary research can progress were introduced and evaluated. The Evoltree network and conference series provides invaluable opportunities to share knowledge and increase collaboration on forest genetic research, the need for which has never been greater or more urgent

    Tailoring conservation to suit Serbian spruce

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    Mediterranean forest tree species are particularly sensitive to the global climate warming, and two Mediterranean conifers, Sicilian fir and Serbian spruce, stand out as currently the most threatened ones due to their extremely narrow distribution range. Both species are IUCN-red listed and protected by the law. Conservation of Serbian spruce (Picea omorica) was initiated in 1955, when a rigid conservation strategy was applied, without any intervention allowed in c. 30 remnant populations scattered within an area of c. 200 km2 in the Western Balkans. Such a strategy, implemented without any genetic knowledge on the species, remained to the present days, and the only advancement regarding Serbian spruce conservation over the past 15 years refers to the inclusion of seven populations (four natural populations and three planted stands) into the pan-European network of genetic conservation units (GCUs) for the dynamic conservation of forest genetic resources. In the meanwhile, a drought-induced physiological weakening of Serbian spruce trees followed by infestation of Armillaria ostoyae and, in turn, a rapid dieback of trees has been observed, suggesting that Serbian spruce is losing the race against climate warming. However, at the same time, genetic knowledge on the species improved significantly. Whole-population genetic characterizations provided in-depth understanding of within-population genetic patterns and genetic changes in natural regeneration following disturbances. Therefore, the conditions for geneticallyinformed conservation and, eventually, species genetic rescue have been met, and a ten-year program, worth 10 million EUR and comprising ten work packages, has been formulated and will be presented.Genomics and Adaptation in Forest Ecosystems. Book of Abstracts. EvolTree Conference 2021, 14 – 17 September 2021, Birmensdorf, Switzerlan

    Historia des Bombardements des Weltbekannten und schreklich gefürchteten Raubnestes Algier, wie solches unter'm Komando des hochberühmten Seehelden Don Barcelo im Jahr 1784 ausgeführt wurde

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    HISTORIA DES BOMBARDEMENTS DES WELTBEKANNTEN UND SCHREKLICH GEFÜRCHTETEN RAUBNESTES ALGIER, WIE SOLCHES UNTER'M KOMANDO DES HOCHBERÜHMTEN SEEHELDEN DON BARCELO IM JAHR 1784 AUSGEFÜHRT WURDE Historia des Bombardements des Weltbekannten und schreklich gefürchteten Raubnestes Algier, wie solches unter'm Komando des hochberühmten Seehelden Don Barcelo im Jahr 1784 ausgeführt wurde (1) Titelseite (1) Vorredner (3) Erster Gesang (5) I. (5) II. (11) Zweiter Gesang (17) I. (17) II. (20) Nachredner (28) Ankündigung (1
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