8,504 research outputs found
CHO microRNA engineering is growing up : recent successes and future challenges
No full textmicroRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines
AVERAGE SHADOW PRICES IN MATHEMATICAL-PROGRAMMING
No full textWe develop the concept of average shadow price in mathematical programming. This concept measures the value of resources along a direction in an average sense, in contrast to traditional marginal analysis; it serves as a useful standard price for management decisions about resources, particularly when there are nonconvexities. We give it an economic interpretation. We also develop simple computational schemes for obtaining and improving the bounds of the average shadow prices and illustrate them in two important classes of nonconvex programs: convex maximization problems and mixed integer programs
Real-time sonar image enhancement for AUV-based acoustic vision
No full textSonar imaging is considered the only feasible sensing solution for underwater investigations by autonomous underwater vehicles (AUVs) in zero-visibility water conditions. State-of-the-art forward-looking imaging sonars can acquire high resolution images at high frame rates, making the acquired images nearly similar to video images acquired using optical cameras. However, the sonar images are corrupted with speckle noise. In this paper, a speckle noise reduction algorithm is proposed for AUV operation. Unlike the conventional algorithms, the proposed algorithm is adequate for real-time treatment. In order to evaluate the performance of the proposed algorithm, an experiment was conducted by using a forward-looking imaging sonar. Based on the experimental results, it was confirmed that the proposed algorithm can effectively reduce the noise. (C) 2015 Elsevier Ltd. All rights reserved.1166sciescopu
cDNA cloning and characterization of an antimicrobial peptide from earthworm. Lumbricus rubellus
No full textUse of a flat-panel detector for microtomography: A feasibility study for small-animal imaging
No full textWe have applied a flat-panel detector to an X-ray cone-beam micro computed tomography (micro-CT) for small-animal imaging. The flat-panel detector consists of an active matrix of transistors and photodiodes with a pixel pitch of 50 mu m and a thallium-doped cesium iodide (CsI:Tl) scintillator as an X-ray-to-light conversion layer. The detector was fabricated with a complementary metal-oxide-semiconductor (CMOS) technology capable of a submicrometer design line width, hence, it has a pixel fill-factor as high as similar to 80%. In addition, the detector has a very fast response characteristic with an image lag less than 0.3% in the frame integration time of 5 s. The CMOS flat-panel detector has been tested in terms of modulation transfer function, noise power spectrum, and detective quantum efficiency. Tomographic imaging performances of the micro-CT system, such as voxel noise, contrast-to-noise ratio, and spatial resolution, have also been evaluated by using various quantitative phantoms. Experimental results of euthanized laboratory rat imaging suggest that the micro-CT system employing a CMOS flat-panel detector can be greatly used in small-animal imaging
A SIMPLIFIED CROSS DECOMPOSITION ALGORITHM FOR MULTIPLE RIGHT HAND CHOICE LINEAR-PROGRAMMING
No full textVARIABLE TARGET VALUE SUBGRADIENT METHOD
No full textPolyak's subgradient algorithm for nondifferentiable optimization problems requires prior knowledge of the optimal value of the objective function to find an optimal solution. In this paper we extend the convergence properties of the Polyak's subgradient algorithm with a fixed target value to a more general case with variable target values. Then a target value updating scheme is provided which finds an optimal solution without prior knowledge of the optimal objective value. The convergence proof of the scheme is provided and computational results of the scheme are reported
Measurement of isomeric yield ratios for the Sc-44m,Sc-g isomeric pairs produced from Sc-45 and Ti-nat targets at 50-, 60-, and 70-MeV bremsstrahlung
No full textWe measured the isomeric yield ratios for the Sc-44m,Sc-g isomeric pairs produced from different reaction channels Sc-45(gamma,n)Sc-44m,Sc-g and Ti-nat(gamma,xnp)Sc-44m,Sc-g by using the activation method and gamma-ray spectroscopic methods at 50-, 60-, and 70-MeV bremsstrahlung energies. The high-purity natural Sc and Ti foils in disc shape were irradiated with uncollimated bremsstrahlung beams generated from an electron linear accelerator at Pohang Accelerator Laboratory. The induced activities in the irradiated foils were measured by the high-resolution gamma-ray spectrometric system which consists of a high-purity Germanium detector and a multichannel analyzer. In order to improve the accuracy of the experimental results the necessary corrections were made in the gamma-ray activity measurements and data analysis. The measured isomeric yield ratios for the Sc-45(gamma,n)Sc-44m,Sc-g reaction are 0.20 +/- A 0.02, 0.21 +/- A 0.02, and 0.21 +/- A 0.02 and those for the Ti-nat(gamma,xnp)Sc-44m,Sc-g reaction are 0.063 +/- A 0.012, 0.079 +/- A 0.014, and 0.124 +/- A 0.022 at 50-, 60-, and 70-MeV bremsstrahlung energies, respectively. The obtained results are compared with the corresponding values found in the literature. We observed that the isomeric yield ratios for the Sc-45(gamma,n)Sc-44m,Sc-g reaction increase rapidly with the increasing bremsstrahlung energies from the reaction threshold up to giant resonance region, and then those are almost constant in the energy range from about 30 to 2.5 GeV. The isomeric yield ratios for the Ti-nat(gamma,xnp)Sc-44m,Sc-g reaction increase with increasing bremsstrahlung energies in a wide range of bremsstrahlung energies from 50 to 2.5 GeV.X1149sciescopu
Use of a Flat-Panel Detector for Microtomography: A Feasibility Study for Small-Animal Imaging
No full textDevelopment of hovering type AUV "Cyclops" and its performance evaluation using image mosaicing
No full textIn this paper, a hovering-type autonomous underwater vehicle called Cyclops is introduced. Because of the symmetric body structure and thruster configuration of Cyclops, it is specially designed to utilize a lawnmower trajectory without changing its heading direction. This movement is effective at reducing the dead reckoning error and obtaining source images with homogeneous optical characteristics for underwater image mosaicing. (C) 2015 Elsevier Ltd. All rights reserved.1116sciescopu
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