2,743 research outputs found
Analysis of enzymatic L/D-peptide isomerization in animals
No full textThe study of cell-to-cell signaling peptides contributes to the fundamental understanding of physiological processes and improves the effectiveness of pharmaceutical drugs. While protein synthesis in animals is stereospecific for all L-form amino acids, a unique and bioactive group of D-amino acid-containing peptides (DAACPs) have been found to exist in a broad array of animals. DAACPs result from a post-translational modification (PTM) in which an L-amino acid residue is enzymatically converted into a D-amino acid residue in the peptide chain. Isomerization leads to important changes in the three-dimensional structure and bioactivity of the DAACP and increases its resistance to degradation by peptidases. However, current research into peptide isomerization is limited because this PTM is not easily detected in mass spectrometry-based peptidomics experiments due to the lack of an associated mass shift. To address this challenge, we have developed a method for the non-targeted detection of DAACPs in biological samples and used it to explore the prevalence of DAACPs in the neurobiological model organism Aplysia californica. Two novel DAACPs were discovered using this method. Based on these results, it was hypothesized that an L/D-peptide isomerase exists in Aplysia which is responsible for the biosynthesis of DAACPs. An assay for isomerase activity was developed and used to partially purify and characterize an L/D-isomerase in the Aplysia central nervous system. Furthermore, isomerase activity was detected in multiple tissues in the rat, and a mammalian neuropeptide was found to be isomerized by this activity. These findings advance our understanding of the extent and function of enzymatic L/D-peptide isomerization as a PTM in bioactive peptides.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-08-01The student, Hua-Chia Tai, accepted the attached license on 2018-07-06 at 17:10.The student, Hua-Chia Tai, submitted this Dissertation for approval on 2018-07-06 at 17:12.This Dissertation was approved for publication on 2018-07-10 at 09:41.DSpace SAF Submission Ingestion Package generated from Vireo submission #12763 on 2018-09-27 at 11:36:22Made available in DSpace on 2018-09-27T16:47:26Z (GMT). No. of bitstreams: 2
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Previous issue date: 2018-07-10Embargo set by: Seth Robbins for item 107897
Lift date: 2020-09-27T16:47:41Z
Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemLimited Restriction Lifted for Item 107897 on 2020-09-28T09:15:16Z
Groundwater and surface water interactions in Fifteenmile Creek Watershed, Oregon - results and analysis from the 2008 seepage run
No full textby Jonathan L. La Marche, PE, and Robert L. Wood.Title from PDF title page (viewed on October 8, 2018)."Draft"--WatermarkThis archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (pages 27-29).Mode of access: Internet from the Oregon Government Publications Collection.Text in English
Focused Laser-Induced Marangoni Dewetting for Patterning Polymer Thin Films
No full textHighly-localized focused laser spike (FLaSk) heating of polymer thin films is a resist- and developer-free alternative to 2D laser direct write for creating patterns on the single micron or, by exploiting overlap effects, submicron scale. The massive temporal and spatial thermal gradients and resulting thermal Marangoni stresses generated by FLaSk are an effective means for the directed dewetting and patterning of such films. Here, the general applicability of this technique to glassy amorphous polymer thin film systems is investigated through systematic investigation of film thickness, glass transition temperature, and polymer mobility. The results reveal that the important parameters are the film thickness (coupled to the optical heating effects through anti-reflection coating effects) and the high-temperature polymer melt mobility, allowing for generation of single features with linewidths of down to ~1 μm. Further, the introduction of spatial mobility variations by using polymer brushes, bilayers, and microphase separated block copolymers leads to additional profile manipulation effects (i.e. spontaneous 2D pattern generation and flattened top profiles).Peer reviewe
Soluble Extracts from Chia Seed (Salvia hispanica L.) Affect Brush Border Membrane Functionality, Morphology and Intestinal Bacterial Populations In Vivo (Gallus gallus)
No full textThis study assessed and compared the effects of the intra-amniotic administration of various concentrations of soluble extracts from chia seed (Salvia hispanica L.) on the Fe and Zn status, brush border membrane functionality, intestinal morphology, and intestinal bacterial populations, in vivo. The hypothesis was that chia seed soluble extracts will affect the intestinal morphology, functionality and intestinal bacterial populations. By using the Gallus gallus model and the intra-amniotic administration approach, seven treatment groups (non-injected, 18 Ω H2O, 40 mg/mL inulin, non-injected, 5 mg/mL, 10 mg/mL, 25 mg/mL and 50 mg/mL of chia seed soluble extracts) were utilized. At hatch, the cecum, duodenum, liver, pectoral muscle and blood samples were collected for assessment of the relative abundance of the gut microflora, relative expression of Fe- and Zn-related genes and brush border membrane functionality and morphology, relative expression of lipids-related genes, glycogen, and hemoglobin levels, respectively. This study demonstrated that the intra-amniotic administration of chia seed soluble extracts increased (p < 0.05) the villus surface area, villus length, villus width and the number of goblet cells. Further, we observed an increase (p < 0.05) in zinc transporter 1 (ZnT1) and duodenal cytochrome b (Dcytb) proteins gene expression. Our results suggest that the dietary consumption of chia seeds may improve intestinal health and functionality and may indirectly improve iron and zinc intestinal absorption
Coos Bay tsunami modeling: toward improved maritime planning response
No full textby Jonathan C. Allan, Joseph Zhang, Fletcher E. O'Brien, and Laura L. S. Gabel.Title from PDF cover (viewed on September 10, 2020).This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (pages 74-78).Mode of access: Internet from the Oregon Government Publications Collection.Text in English
Tsunami evacuation analysis of Nehalem Bay, Tillamook County, Oregon
No full textby Laura L. S. Gabel, Fletcher O'Brien, and Jonathan Allan.Title from PDF cover (viewed on September 10, 2020).This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (pages 51-53).Mode of access: Internet from the Oregon Government Publications Collection.Text in English
GIS data and method for determining maximum-considered local and distant tsunami wave arrival data for the Oregon coast
No full textby Laura L. S. Gabel, Fletcher O'Brien, and Jonathan C. Allan.Title from PDF cover (viewed on September 10, 2020).This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (pages 22-23).Mode of access: Internet from the Oregon Government Publications Collection.Text in English
Tsunami evacuation analysis of Gold Beach and nearby unincorporated communities, Curry County, Oregon
No full textby Laura L. S. Gabel, Fletcher O'Brien, and Jonathan Allan.Title from PDF cover (viewed on May 27, 2021).This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (pages 69-71).Mode of access: Internet from the Oregon Government Publications Collection.Text in English
Earthquake and tsunami impact analysis of five Oregon coastal communities
No full textReport -- Spreadsheet.by John M. Bauer, Jonathan C. Allan, Laura L. S. Gabel, Fletcher E. O'Brien, and Jed T. Roberts.Title from PDF cover (viewed on July 7, 2020).This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references.Mode of access: Internet from the Oregon Government Publications Collection.Text in English
Tsunami evacuation analysis of Port Orford, Curry County, Oregon
No full textThis report shows modeled pedestrian evacuation routes to escape a local tsunami generated by an earthquake on the Cascadia Subduction Zone (CSZ) for the community of Port Orford, Curry County.by Laura L. S. Gabel, Jonathan C. Allan, and Fletcher E. O'Brien.Title from PDF cover (viewed on July 8, 2020).This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (pages 59-61).Mode of access: Internet from the Oregon Government Publications Collection.Text in English
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