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    Dentin Permeability in vitro after application of tartaric acid solutions

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    Preliminary investigations demonstrated that salts tartrate solutions are able to create a layer of non-homogeneous crystals able to close dentainal tubules and reduce fluid flow rate calculated using a pressur apparatus. The aim of this study was to evaluate the ability of a new solution constitued by tartaric acid (TA) solutions 0.1M (Ph 3.5) to reduce in vitro the rate of fluid flow, also defined as dentin permeability (Lp). Dentin disc from human molars (N=48) were prepared and treated with 1m edta for 2 mins to remove the smearl layer and to calculate the maximum rate for each disc. The discs were connected with an hydraulic pressure apparatus working at 0.5 psi(Pashley, 1984). Fluid flow of 100% was assigned (Group A). A new smearl layer was re-created only in 24 samples (Group B). Solutions were applied for 2 mins and gently washed with water for 30 seconds, and fluid flow rate re-measured. EDTA was then re-applied for 2 mins, washed and fluid flow re-calculated. SEM analysis was performed for several samples. Results and Conclusions: The application of tartrate solution reduced the fluid flow rate (p<0.0001), and modified smearl layer morphology. These treatments my contribute the dentin protection from diet acid attacks and may reduce dentin hypersensitivity

    In vivo effects of two in-office vital tooth bleaching systems on enamel permeability

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    Tooth bleaching is a common treatment for the amelioration of the aesthetic of discoloured teeth. In this context, there are two common approaches that employ concentrated solutions (30–40 wt.%) of either hydrogen peroxide or carbamide peroxide as bleaching agents. However, there is an ongoing debate on the possible adverse effects of these different treatments on tooth health, such as variation of the enamel structure, surface morphology, and chemistry, which also affect tooth sensitivity. In the present work, a study on the effect of the two bleaching agents, a 35 wt.% solution of hydrogen peroxide and a 30 wt.% solution of carbamide peroxide, on the permeability and surface morphology of enamel is reported. The investigation was carried out on replicas of incisors obtained after different treatment times and for several patients, employing scanning electron microscopy to study the morphological features of the treated teeth. The significance of the analytical study was corroborated by a statistical analysis of the results. The collected data suggest that hydrogen peroxide treatment increases the enamel permeability, and this could be related with tooth sensitivity, whereas the carbamide peroxide solution increases the formation of precipitates on the tooth enamel

    Effects of different acid and sodium hypochlorite treatments on dentin collagen: A FEISEM analysis.

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    Purpose:To evaluate if different acid treatments of dentin surface might remove different amount of mineralized dentin, thus exposing and modifying the CF network. METHODS: Dentin disks prepared from human third molars with a low-speed diamond saw were etched for 15 seconds with the tested acids: citric acid 10%, maleic acid 10%, 2.5% oxalic acid, 35% phosphoric acid and 24% EDTA gel. Specimens were then submitted to a 5% solution of NaOCl for 2 minutes or 5 minutes, fixed, and observed utilizing a field emission in lens SEM (FEISEM). Control specimens were also prepared by omitting the etching agent and/or the NaOCl solution. RESULTS: The different acid treatments created specific dentin morphological pattern. CF exposure was in relationship with the acid used. The NaOCl solution greatly affects the acid etched dentin by removing the CF, nevertheless the effect of NaOCl was greatly influenced by the previous acid treatment

    Hydraulic Conductance in Vitro of Human Dentin after application of Tartrate Solutions.

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    Hydraulic Conductance in Vitro of Human Dentin after application of Tartrate Solutions. The aim of this study was to evaluate the ability of two solutions 0.1M NaKC4H406 and (CH3COO)2Ca to reduce in vitro the rate of fluid flow, also defined as dentin permeability (Lp). Dentin disc from human molars (N=48) were prepared and treated with 35% phosphoric acid for 2 mins to remove the smearl layer. The discs were connected with an hydraulic pressure apparatus working at 0.5 psi(Pashley, 1984). Fluid flow of 100% was assigned (Group A). A new smearl layer was re-created only in 24 samples (Group B). Solutions were applied for 2 mins and gently washed with water for 30 seconds, and fluid flow rate re-measured. Phosphoric acid was then re-applied for 2 mins, washed and fluid flow re-calculated.SEM analysis was performed for several samples. Results and Conclusions: The application of tartrate solution did not reduced the fluid flow rate, but prevented the removal of smearl layer by the second acid attack. These treatments my contribute the dentin protection from diet acid attacks
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