1,721,047 research outputs found
The arsenic hyperaccumulating Pteris vittata expresses two arsenate reductases
Full text linkEnzymatic reduction of arsenate to arsenite is the first known step in arsenate metabolism in all organisms. Although the presence of one mRNA arsenate reductase (PvACR2) has been characterized in gametophytes of P. vittata, no arsenate reductase protein has been directly observed in this arsenic hyperaccumulating fern, yet. In order to assess the possible presence of arsenate reductase in P. vittata, two recombinant proteins, ACR2-His6 and Trx-His6-S-Pv2.5-8 were prepared in Escherichia coli, purified and used to produce polyclonal antibodies. The presence of these two enzymes was evaluated by qRT-PCR, immunoblotting and direct MS analysis. Enzymatic activity was detected in crude extracts. For the first time we detected and identified two arsenate reductase proteins (PvACR2 and Pv2.5-8) in sporophytes and gametophytes of P. vittata. Despite an increase of the mRNA levels for both proteins in roots, no difference was observed at the protein level after arsenic treatment. Overall, our data demonstrate the constitutive protein expression of PvACR2 and Pv2.5-8 in P. vittata tissues and propose their specific role in the complex metabolic network of arsenic reduction
Micorrize arbuscolari come strumento biotecnologico nel risanamento da idrocarburi aromatici
No full textA novel chimeric cellulase by combining CelA of Dictyoglomus turgidum with the carbohydrate binding module CBM11 from Clostridium thermocellum
No full textCostruzione di biodegradatori fungini dei composti aromatici appartenenti al complesso BTEX (Benzene, Toluene, Etil-benzene, Xilene)
No full textAssessing gradients of genotossicity in polluted soils with Comet-test using Pisum sativum, a case study from a polluted site
No full textAssessing gradients of genotossicity in polluted soils with comet test using Pisum sativum L clones: a case study from a polluted site. EURAC book; Analisi Spazio Temporali: dinamiche e processi a confronto
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System toxicology: la proteomica nella valutazione delle sostanze chimiche in organismi animali e vegetali
No full textCross-species identification in plant proteomics: comparison of Protein Pilot and Mascot softwares for the analysis of MS/MS data
No full textUse of arbuscular mycorrhizal fungi for the bioremediation of monoaromatic hydrocarbon polluted sites.
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