1,721,060 research outputs found
FIGURE 3. A in An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens
No full textFIGURE 3. A: Egg shell of M. terminalis (hologenophore C2868-N02 US2, DIC); B: Egg shell of M. terminalis by SEM (C2868); C: Egg shell of a M. terminalis paratype (C624-S44, PhC); D: Egg shell of M. cf. terminalis (C2341, PhC). Bar =10 µm (A, C, D); 1 µm (B).Published as part of Cesari, Michele, Giovannini, Ilaria, Bertolani, Roberto & Rebecchi, Lorena, 2011, An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens, pp. 42-51 in Zootaxa 3104 (1) on page 48, DOI: 10.11646/zootaxa.3104.1.3, http://zenodo.org/record/524530
Figure 11 in Somatic musculature of Tardigrada: phylogenetic signal and metameric patterns
No full textFigure 11. Phylogenetic tree obtained with Bayesian analyses based on molecular data (18S rRNA + 28S rRNA). The posterior probability values are indicated next to each node.Published as part of Marchioro, Trevor, Rebecchi, Lorena, Cesari, Michele, Hansen, Jesper Guldberg, Viotti, Giulia & Guidetti, Roberto, 2013, Somatic musculature of Tardigrada: phylogenetic signal and metameric patterns, pp. 580-603 in Zoological Journal of the Linnean Society 169 (3) on page 598, DOI: 10.1111/zoj.12079, http://zenodo.org/record/529117
Molecular taxonomy and phylogeny in the "living fossil" lineages Triops and Lepidurus (Branchiopoda: Notostraca).
No full textIdentification of the trehalose-6-phosphate synthase (tps) gene in desiccation tolerant and intolerant tardigrades
No full textThe presence of the gene coding for trehalose-6-phosphate synthase (tps) was determined for the first time in Tardigrada in twelve species belonging to both Heterotardigrada and Eutardigrada. The tps gene was found in all semiterrestrial and anhydrobiotic species examined, correlating trehalose presence with anhydrobiotic ability. In contrast, tps was not detected in two limnic and desiccation intolerant species. The surprising presence of tps in another limnic and desiccation intolerant species, Dactylobiotus parthenogeneticus, allowed us to infer that in tardigrades trehalose could be produced and involved, not just in anhydrobiosis, but also in the regulation of other biological functions, such as encystment. Our findings, integrated with literature data, indicate that anhydrobiotic ability in tardigrades can depend on the combined action of numerous different molecules, only partially identified. With nucleotide tps sequences, it is impossible to infer phylogenetic relationships inside the phylum Tardigrada, while amino acid sequences further support the relationship between Tardigrada and Pancrustacea
Fig. 5. Comparison between M in Integrative description of a new Tunisian tardigrade species, Macrobiotus azzunae sp. nov. (Eutardigrada, Macrobiotidae, hufelandi group)
No full textFig. 5. Comparison between M. azzunae sp. nov. and M. sandrae Bertolani & Rebecchi, 1993. A. Placoids in M. azzunae sp. nov., paratype (UNIMORE, slide C4218–S30). B. Placoids in M. sandrae (UNIMORE, slide C442–S79); arrowheads evidence the different constriction depth of the first macroplacoid. C. Eggshell in M. azzunae sp. nov., paratype (UNIMORE, slide C4218–S4). D. Eggshell in M. sandrae (UNIMORE, slide C2346–S2); in M. azzunae sp. nov. there are smaller processes and reticulation with thinner wires and larger net around the processes than in M. sandrae. A–D: PhC.Published as part of Marnissi, Jamila Ben, Cesari, Michele, Rebecchi, Lorena & Bertolani, Roberto, 2021, Integrative description of a new Tunisian tardigrade species, Macrobiotus azzunae sp. nov. (Eutardigrada, Macrobiotidae, hufelandi group), pp. 122-146 in European Journal of Taxonomy 758 (1) on page 136, DOI: 10.5852/ejt.2021.758.1429, http://zenodo.org/record/508810
Figure 2. A, B in Somatic musculature of Tardigrada: phylogenetic signal and metameric patterns
No full textFigure 2. A, B, the eutardigrade Milnesium cf. tardigradum in active state (A, ventral view; B, lateral view). C, D, the eutardigrade Paramacrobiotus richtersi in active state (C; dorsal view) and in dry anhydrobiotic state (D; tun shape). Asterisk indicates front. Letters and numbers identify the muscle attachment points (see text). A–D, SEM. Scale bars: A–C = 50 μm; D = 20 μm.Published as part of Marchioro, Trevor, Rebecchi, Lorena, Cesari, Michele, Hansen, Jesper Guldberg, Viotti, Giulia & Guidetti, Roberto, 2013, Somatic musculature of Tardigrada: phylogenetic signal and metameric patterns, pp. 580-603 in Zoological Journal of the Linnean Society 169 (3) on page 585, DOI: 10.1111/zoj.12079, http://zenodo.org/record/529117
Figure 12 in Somatic musculature of Tardigrada: phylogenetic signal and metameric patterns
No full textFigure 12. Schematic representations of hypothetical ancestral serial homologies of the different muscle groups in tardigrades. A, dorsal muscle group in eutardigrades; B, dorsal muscle group in heterotardigrade echiniscids; C, lateral muscle group in tardigrades; D, ventral muscle group in tardigrades.Published as part of Marchioro, Trevor, Rebecchi, Lorena, Cesari, Michele, Hansen, Jesper Guldberg, Viotti, Giulia & Guidetti, Roberto, 2013, Somatic musculature of Tardigrada: phylogenetic signal and metameric patterns, pp. 580-603 in Zoological Journal of the Linnean Society 169 (3) on page 600, DOI: 10.1111/zoj.12079, http://zenodo.org/record/529117
FIGURE 1. A in An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens
No full textFIGURE 1. A: Fresh specimen of M. terminalis; buccal pharyngeal apparatus with dorsal buccal armature (C2868, DIC); B: Fresh specimen of M. macrocalix; buccal pharyngeal apparatus with dorsal buccal armature (C2868, DIC). C: Claws with indented lunules on a hind leg of M. terminalis (C2868, SEM); D: Claws with indented lunules (arrow heads) on the hind legs of a fresh specimen of M. terminalis (C2868, DIC). Bar =10 µm (A, B, D); 5 µm (C)Published as part of Cesari, Michele, Giovannini, Ilaria, Bertolani, Roberto & Rebecchi, Lorena, 2011, An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens, pp. 42-51 in Zootaxa 3104 (1) on page 46, DOI: 10.11646/zootaxa.3104.1.3, http://zenodo.org/record/524530
FIGURE 2. Thulinius augusti. A in Aquatic tardigrades in the Great Smoky Mountains National Park, North Carolina and Tennessee, U. S. A., with the description of a new species of Thulinius (Tardigrada, Isohypsibiidae)
No full textFIGURE 2. Thulinius augusti. A. Mouth with sub-lobes (arrow). B. Claws II. C. Claws II, cuticular bars under claws (arrows). D. Claws III, cuticular bars under claws (arrow). (PhC; bars = 10µm)Published as part of Bertolani, Roberto, Bartels, Paul J., Guidetti, Roberto, Cesari, Michele & Nelson, Diane R., 2014, Aquatic tardigrades in the Great Smoky Mountains National Park, North Carolina and Tennessee, U. S. A., with the description of a new species of Thulinius (Tardigrada, Isohypsibiidae), pp. 524-536 in Zootaxa 3764 (5) on page 529, DOI: 10.11646/zootaxa.3764.5.2, http://zenodo.org/record/23031
A model study for tardigrade identification
Full text linkUsing tardigrades from a single moss sample as a case study,we propose a new method for tardigrade species identification, which is often problematic, due to the low number of orphological characters. Identification at generic level was carried out on adults, while morphological analyses were performed on animals (LM) and eggs (LM and SEM), including hologenophores, vouchers used also for molecular analysis of COI mtDNA. This multi-approach method revealed the presence of three species of the“Macrobiotus hufelandi group” instead of the two species identified in a previous study. The validity of the method is shown, indicating that it could be applied to studies of problematic meiofauna taxa
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