1,721,266 research outputs found
Epidemiological observation on Chlamydia psittaci infections and disease of animal in Italy
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Tubercolosi e Brucellosi nel Bovino
No full textThe Authors report on epidemiology, prophylaxis and control of tuberculosis and brucellosis in dairy cattle. The main characteristics of these diseases in cattle, the times of survival of brucella and mycobacterium in different substratum and the diagnostic techniques are illustrated The epidemiological situation in Italy and the problems related to the national plans of eradication are finally reported
Cat scratch disease. Survey on the presence of Bartonella henselae among cats of Tuscany
No full textTo verify the presence of Bartonella henselae-infection in cats living in Tuscany (central Italy) serological and bacteriological surveys were carried out. The blood serum samples of 427 cats, 254 living in private houses and gardens and 173 in public or private catteries, were tested for anti-B. henselae antibodies by indirect immunofluorescence assay (IFA). Among these samples, 35 were examined by IFA to detect anti-bodies against Bartonella quintana. Bacteriological examinations were performed on the blood samples, collected in EDTA (ethylene diaminetetraacetic acid), of 18 cats (10 seropositive to B. henselae and 8 negative). From each of the same 18 specimens DNA was extracted and used as template in polymerase chain reaction (PCR). The primers p24E and p12B were employed in the PCR assay to amplify a 296 bp fragment of the Bartonella 16S rRNA gene. IFA detected 98 (22.95%) B. henselae-positive serum samples (40-40.82% from cats living in houses and gardens and 58-59.18% from cats of catteries) at different antibody titers (70 at 1:64 titer, 4 at 1:128, 22 at 1:256, 2 at 1:512). Among the 35 sera tested to detect antibodies against B. quintana, 9 (25.71%) resulted positive at 1:64 titer; all these samples showed higher antibody titers to B. henselae. Out of the 26 negative sera, 20 were negative to B. henselae too and 6 had antibodies against B. henselae at 1:64. Hemocultures gave negative results. PCR scored positive with DNA of 4 B. henselae-seropositive cats, two of which belonged to two children with cat scratch disease (CSD)
Enterobatteri patogeni nel contenuto intestinale e sulla sierosa peritoneale parietale di cinghiali selvatici
No full textIndagine sul potere tossigeno di alcuni stipiti di Yersinia enterocolitica isolati da prodotti carnei trasformati
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