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Immunochemical Methods Applied to Art-Historical Materials: Identification and Localization of Proteins by ELISA and IFM
Despite the large diffusion of natural organic substances in art-historical materials, their characterization presents many challenges due to the chemical complexity and instability with respect to degradation processes. Among natural products, proteins have been largely used in the past as binders but also as adhesives or additives in coating layers. Nevertheless, biological identification of proteins in art-historical objects is one of the most recent achievements obtained in heritage science thanks to the development of specifically tailored bio-analytical strategies. In the context of this active emerging discipline, immunological methods stand out for sensitivity, specificity and versatility for both protein recognition and localization in micro-samples. Furthermore, the growing use of immunological techniques for advanced diagnostics and clinical applications ensures continuous improvement in their analytical performance. Considering such, this review provides an overview of the most recent applications of enzyme linked immunosorbent assay and immunofluorescence microscopy techniques in the field of heritage materials. Specifically, the main strengths and potentials of the two techniques as well as their limits and drawbacks are presented and discussed herein
On the Formation of Nitrogen-Bearing Molecules in Extraplanetary Atmospheres via Neutral Reactions : A Crossed Beam Study of N(2D)+C2H2
Crossed Beam Reactive Scattering of Nitrogen Atoms: the Reaction Dynamics of N( 2 D) + CH 4 and N( 2 D) + H 2 O
Experimental studies on elementary reactions of C atoms and CN and OH radicals with molecules of importance in the interstellat medium
Crossed beam studies of the reaction dynamics of nitrogen atoms with saturated and unsaturated hydrocarbons: N( 2 D)+CH 4 , C 2 H 2 and C 2 H 4
LABORATORY STUDIES ON ELEMENTARY REACTIONS OF C ATOMS AND CN AND OH RADICALS WITH MOLECULES OF IMPORTANCE IN THE INTER-STELLAR-MEDIUM
On the Reaction Dynamics of Electronically Excited 2D Nitrogen Atoms with Polyatomic Molecules : A crossed Beam Study of N(2D)+C2H2 and C2H4
Crossed Beam Reactive Scattering of Nitrogen Atoms: the Reaction Dynamics of N( 2 D) + CH 4 and N( 2 D) + H 2 O
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