1,721,032 research outputs found
Somatic embryogenesis of Vitis vinifera L. (cv Sugraone) from stigma and style culture
No full textSomatic embryo and plant regeneration were induced from stigma and style culture of grapevine (Vitis vinifera L. cv. Sugraone). To obtain somatic embryogenesis, explants were cultured on Nitsch and Nitsch basal medium (NN) supplemented with 88 mM sucrose and various combinations of the auxin ?-naphthoxyacetic acid (NOA, 0-10 ?M) and the cytokinin 6-benzylaminopurine (BA, 0-9 ?M). Growth regulators (BA and NOA) in the culture medium were essential for induction of somatic embryogenesis since explants incubated on hormone-free medium never regenerated somatic embryos. Usually, the regenerated somatic embryos become visible as small white globular structures on the surface of the callus 3-4 months after culture initiation. The best embryogenic response (27 %) was obtained when stigma and style explants were cultured on NN medium supplemented with 5 ?M NOA and 9 ?M BA. Somatic embryos developed into plantlets when transferred to a hormone-free semisolid NN medium. 35 % of primary somatic embryos showed secondary embryogenesis
The Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) and its Role in Conservation of Cacti and Other Succulent Plants.
No full textConservazione della biodiversità e tutela dei valori eco sistemici nelle piccole isole circumsiciliane.
No full text
Effect of 2,4-D and 4-CPPU on somatic embryogenesis from stigma and style transverse thin cell layers of Citrus
No full textCallus induction, somatic embryogenesis and plant regeneration were obtained in lemon [Citrus limon (L.) Burm. cv `Femminello'] and sweet orange [C. sinensis (L.) Osb. cv `Washington Navel GS'] from cultures of stigma and style transverse thin cell layer explants [(t)TCLs]. Explants were cultured on 16 different media, based on the nutrients and vitamins of Murashige and Tucker medium (MT) supplemented with different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and N-(2-chloro-4-pyridyl)-Nprime-phenylurea (4-CPPU). Sucrose (146 mM) was used as the sole carbon source. Somatic embryos arose from callus at the surface of stigma and style (t)TCLs 3–5 months after culture initiation of both sweet orange and lemon. The percentages of embryo formation from style (t)TCLs ranged from 0% (the media containing 2,4-D) to 16.0% (the medium supplemented with 4 mgrM 4-CPPU) for C. limon. Better results were obtained when stigma (t)TCLs from C. limon were used; in fact, percentages ranged from 0% on the media containing 2,4-D, with the only exception for the medium supplemented with 0.4 mgrM 2,4-D, to 24.8% on medium with 4 mgrM 4-CPPU. The embryogenic response of lemon (t)TCLs was usually higher than for sweet orange (t)TCLs. After about 3 months, somatic embryos developed into plantlets at high frequencies ranging from 53% to 75% for sweet orange and lemon style derived embryos, respectively
Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon).
No full textCallus induction, somatic embryogenesis and plant regeneration were obtained in Citrus limon (L.) Burm. (cv. Femminello) from cultures of pistil transverse thin cell layer explants [(t)TCLs]. Explants were cultured on two different media, based on Murashige and Skoog salts and vitamins, supplemented with 500 mg l−1 malt extract (MSI), or 500 mg l−1 malt extract and 13.3 μM 6-benzylaminopurine (MSII). Sucrose (146 mM) was used as carbon source. Somatic embryos appeared 3 months after culture initiation from stigma and style (t)TCLs; they were observed at the surface of the (t)TCL-derived callus. Although ovary (t)TCLs showed the highest callus formation, they never differentiated somatic embryos. Percentages of embryo formation from (t)TCLs incubated on MSI (13% and 2% for stigma and style, respectively) were lower than those from (t)TCLs incubated on MSII (36% and 7% for stigma and style, respectively). The embryogenic response of stigma (t)TCLs was usually higher than that of style (t)TCLs. After about 3 months, somatic embryos developed into plantlets at high frequencies (57% and 62% for stigma- and style-derived somatic embryos, respectively)
Inflorescence scents of Calendula maritima, C. suffruticosa subsp. fulgida, and their hybrid
No full textPremise of research. Hybridization is an important driver of plant evolutionary processes. By attracting the same pollinators to different species, floral scents may be involved in the formation of hybrids and breakdown of species boundaries. In contrast, by attracting a different suite of pollinators to hybrids and their parents, floral scents are believed to contribute to speciation processes initiated by hybridization events. Scents may or may not differ between the hybrids and their parents, but little is known about the scent chemistry of parental species and their hybrids.
Methodology. We studied the inflorescence scents of parental Calendula maritima and C. suffruticosa subsp. fulgida (henceforth, C. fulgida) and a morphologically intermediate hybrid. Scents were collected by dynamic headspace and analyzed by thermal desorption-gas chromatography/mass spectrometry. Insects visiting inflorescences of the three taxa were also captured.
Pivotal results. Calendula maritima and C. fulgida emitted different absolute amounts of scent; the hybrid released intermediate amounts of scent. The scents of the parental and hybrid taxa were all dominated by monoterpenes, with several compounds in common among the taxa. Nevertheless, the three taxa showed differences in qualitative and semiquantitative scent patterns. Calendula maritima emitted more and a higher amount of sesquiterpenes than the other taxa. The hybrid was overall more similar in scent properties to C. fulgida than it was to C. maritima.
Conclusions. The overlap in scent compounds among the taxa may be responsible for attraction of the same insect pollinators, resulting in interspecific pollen transfer between the parents and the formation of hybrids. Indeed, preliminary observations revealed that all three taxa are visited by Panurgus siculus bees (Andrenidae)
- …
