1,721,124 research outputs found
Comparative investigation of two nuclear isoforms of carrot DNA topoisomerase I:role in cell growth.
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Antisense RNA strategy applied to carrot top1a and b genes as a new tool for investigating the apoptotic response in plant cells
No full textAntisense down-regulation of carrot top1a and top1b genes coding for topoisomerases I leads to delayed entry into PCD induced by reactive oxygen species.
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Nuclease-producing bacteria in soil cultivated with herbicide resistant transgenic white poplars
No full textThis study was carried out using soil cultivated, under greenhouse conditions, with transgenic white poplars expressing the
bar gene for tolerance to the Basta® herbicide. The occurrence of extracellular nucleolytic activity was monitored in soil samples collected
at four different times over a 26-month period. The fraction of nuclease producing bacteria (NPB) ranged from 62.5 to 100%
of the total culturable bacterial population. The DNA-methyl green plate assay allowed to distinguish five groups of bacteria showing
increasing levels of extracellular DNase activity. The NPB isolates were classified by 16S rDNA sequence analysis as members of the
Bacillus, Brevibacillus, Microbacterium, Pseudomonas and Stenotrophomonas genera. For each genus, NPB isolates were cultured in
liquid medium and the nucleolytic activity during different growth phases was monitored. Production of extracellular nucleases was
observed only during the mid-exponential growth phase of the Brevibacillus, Microbacterium and Stenotrophomonas isolates, while no
activity was evidenced for isolates classified within the Bacillus and Pseudomonas genera
Requirement for topo Ia or topo Ib expression for plant cell viability revealed by antisense RNA strategy
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