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Morphological characterization by SEM of Psoralea corylifolia and Psoralea drupacea fruits
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Leaf surface morphology of Atropa belladonna and of some adulterant species by scanning electron microscopy.
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Embriogenesi somatica in alcune specie rare ed a rischio di estinzione: prospettive e applicazioni
No full textThe Botanical Garden of Padua is engaged on developing in vitro methologies for long-term storage of germplasm collections of endangered plants. In vitro culture techniques have been developed for both conservation purposes and production of improved somaclones of medicinal plants. In this work, we describe the somatic embryogenesis of rare and threatened native plants from North Eastern Italy and the production of synthetic seeds via the encapsulation of somatic embryos for biodiversity conservation purposes (cryopreservation). Moreover, we report the somatic embryogenesis from sterile plantlet explants of the high alkaloid content VV27 somaclone of Catharanthus roseus (L.) G. Don previously obtained by indirect somatic embryogenesis
Organogenesis from shoot segments and via callus of endangered Kosteletzkya pentacarpos (L.) Ledeb.
No full textEfficient plant regeneration systems both from shoot segments and via callus organogenesis were developed for Kosteletzkya pentacarpos (L.) Ledeb., a rare and endangered Eurasian species. In the experiments with existing meristems, factors affecting shoot proliferation, including explant type, i. e. decapitated and intact shoots, and plant growth regulators, indole-3-acetic acid or kinetin, were investigated. Shoot proliferation was significantly affected by the type of explant, the hormones and their interaction. The highest shoot multiplication rate was obtained from decapitated shoots. Increasing kinetin concentration promoted shoot elongation regardless of explant type. In intact shoots, shoot length was also affected by increasing auxin concentration, although this effect tends to decrease with higher concentration. Decapitated shoots were not responsive to the addition of auxin. Micropropagation through organogenesis from callus was also investigated. Calli were obtained from leaf, stem internode and root explants. Only the leaf-derived calli produced shoots and indole-3-acetic acid favoured increased numbers of shoots. A number of experiments were conducted for rooting of in vitro produced shoots. All of them induced high rooting frequency, the number and the length of roots being dependent on the strength of the basal medium. The use of 1-2 mg l-1 indole-3-butyric acid resulted in refining the optimal concentration for root elongation. The regenerated plants (70%) survived and flowered in their first vegetative perio
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