1,722,938 research outputs found
Characterization of a Ca2+-stimulated Mg2+-dependent adenosine triphosphatase in Friend murine erythroleukemia cell plasma membranes.
No full textSince agents which affect Ca2+ fluxes have been shown to affect the commitment to terminal differentiation of murine erythroleukemia cells treated with an inducer of differentiation (Bridges, K., Levenson, R., Housman, D., and Cantley, L. (1981) J. Cell Biol. 90, 542-544), we investigated the presence of Ca2+-transport systems in plasma membranes isolated from these cells. Plasma membranes from murine erythroleukemia cells exhibited a high affinity (Ca2+-Mg2+)-ATPase activity with a Vmax of 29 +/- 4 nmol/mg/min and an apparent K0.5 for free Ca2+ of 0.13 microM. This activity was strongly inhibited in a dose-dependent fashion by low concentrations of trifluoperazine, compound 48/80, lanthanum, and vanadate with I50 of 29 microM, 1.75 micrograms/ml, 1.5 microM, and 0.4 microM, respectively. The inhibitory effect of compound 48/80 was specifically reversed by exogenously added calmodulin. Phosphorylation of plasma membranes with [gamma-32P] ATP followed by sodium dodecyl sulfate electrophoretic analysis at low pH revealed a Ca2+-dependent phosphoprotein with an apparent molecular mass of 138,000 daltons which co-migrated with the Ca2+-dependent phosphoprotein from human erythrocytes and was separable from sarcoplasmic reticulum (Ca2+-Mg2+)-ATPase. The 32P incorporated into the phosphoprotein could be chased with unlabeled ATP and the protein-phosphate bond was unstable at alkaline pH suggesting an acylphosphate ATPase intermediate like that previously characterized in other (Ca2+-Mg2+)-ATPases
James Cantley (1896-1969)
Full text linkOn August 7, 1969, at the age of 73, James Cantley died, ... Some 56 years ago in the summer of 1913 ..., not yet out of his teens, ["Jim" Cantley] ... stepped ashore at Cartwright, Labrador, off the Pelican, the last of the Hudson\u27s Bay Company\u27s sailing ships. ... During his early years fur trading in Arctic Canada by small boats and dog teams he travelled extensively through the eastern Arctic. He carried out exploratory work on the east coast of Baffin Island in search of promising locations for Hudson\u27s Bay Company trading posts. He established in 1914 the first post at Ward Inlet, Frobisher Bay, some 40 miles from the present site of the community, which has now become the main centre for the eastern Arctic. This was at a period when truly Arctic posts designed specifically to trade with the Eskimos for white foxes were being opened in the Arctic. ... In 1921 Jim Cantley was transferred south and appointed District Accountant and later Assistant District Manager of the Hudson\u27s Bay Company\u27s eastern operations with headquarters first at St. John\u27s, Newfoundland and later at Montreal. In 1930 he moved to Winnipeg as Assistant Fur Trade Commissioneer and during the next eight years he made numerous trips throughout the Northwest Territories and the northern parts of all the provinces from coast to coast. He left the Hudson\u27s Bay Company in 1938 and the following year organized and, for the next ten years, managed the Baffin Trading Company Limited which was engaged in trading and transportation in the eastern Arctic. In 1950 he joined the Department of Indian Affairs and Northern Development .... Part of his work was to carry out a survey of economic conditions in the Arctic. ... he retired from the Department in 1956. In 1957 he was appointed a Fellow of the Arctic Institute of North America and around that time he helped set up and was the Director of the Ottawa office of the Institute. With the closing of this office, Jim Cantley continued his association with the north in one way and another, corresponding with many people who were doing northern research. He also served the Arctic Circle Club for many years as one of the auditors
Biotechnology : The challenge to Europe
No full textCantley Mark, Sargeant Ken. Biotechnology : The challenge to Europe . In: Revue d'économie industrielle, vol. 18, 4e trimestre 1981. Genèse et développement de la BIOINDUSTRIE. pp. 319-334
Mechanisms and control of pathologic bone loss in periodontitis
No full textP. Mark Bartold, Melissa D. Cantley and David R. Hayne
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