44 research outputs found
Development of nanostructured biosensors by means of the Layer by Layer Self-Assembly technique
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Nanostructured Thin Films for the Development of Piezoelectric Immunosensors
No full textMonoclonal antibodies were immobilized onto the surface of quartz crystals for the development of piezoelectric biosensors by means of the Layer by Layer self assembly technique (LBL). Specifically, the immobilization of immunoglobulins specific to the human cytokine Transforming Growth Factor Beta1 and to taxol was investigated. To this purpose multilayered ultra-thin films composed by precursor layers of cationic poly(dimethyldiallylammonium) chloride and anionic poly(styrenesulfonate) followed by a monolayer of antibodies were assembled by LBL. A quartz crystal microbalance was used to monitor and optimize the assembly process and to test the immunological activity of the deposited antibody molecules. Atomic force microscopy was used to characterize the surface roughness of the multilayers before and after the deposition of the immunoglobulins. The results confirmed the successful deposition of the proposed immunosensors and demonstrated their high potential for the measurement of analytes of clinical interest
Human osteoblast-like cells response to nanofunctionalized surfaces for tissue engineering.
No full textCells are sensitive both to the micro/nanotopographic and chemical features of their surrounding environment. The engineering of the surface properties of biomaterials is then critical to develop bioactive devices with which to elicit appropriate cellular responses. To this regard, the layer by layer (LBL) self assembly technique represents a simple and versatile method to modify surface properties by the deposition of ultrathin films with specific and predetermined properties. In this work biomimetic coatings containing fibronectin, an adhesive glycoprotein of the extracellular matrix, were assembled by means of the LBL technique, and tested for the growth of MG63 human osteoblast-like cells, in order to evaluate their potential for the treatment of materials employed in bone-tissue engineering. As a first step the assembly process was optimized by quartz crystal microbalance measurements and subsequently was repeated on nickel/titanium, silicon and glass samples. The results obtained from the investigation of cell response to the modified surfaces, put in evidence that the deposited nanostructured ultrathin films are effective in promoting cell proliferation. Our results show the high potential of the developed bioactive coatings for the engineering of biomimetic implants and for the optimization of their integration with the surrounding tissues
Fabrication and characterization of carbon nanotubes based coatings for the repair of cartilage tissue
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Nanofunctionalisation for the Treatment of Peripheral Nervous System Injuries
No full textA construct based on the electrostatic layer-by-layer self assembly technique has been fabricated, to be used as a tailored device to encourage nerve regeneration. A multilayered nanocoating composed by three precursor bilayers of cationic and anionic polyelectrolytes followed by bilayers of poly-D-lysine (PDL) and antibody specific to transforming growth factor beta1 (anti-TGF-beta1) has been deposited on HYAFF 11reg. Initially the assembly process has been monitored by quartz crystal microbalance (QCM) in order to select the optimal working conditions for nanocoating deposition. Structural studies of the resulting multilayers confirmed stepwise deposition of anti-TGF-beta1 with an average layer thickness of 2.6 nm and an average layer mass of 117 ng. Atomic force microscopy has been used to characterize multilayer uniformity. Finally, the immunological activity of the multilayered structure has been assessed. The results show that anti-TGF-beta1 can be included in its active form in a predetermined multilayered structure onto HYAFF11reg with quantitative control of layer thickness and weight, providing a high potential tool in tissue engineerin
