1,721,085 research outputs found
Fotosintesi
No full textLa vita sulla Terra è strettamente legata e dipendente dall’energia del Sole.
Il nostro pianeta è immerso nella luce e gli organismi che l’hanno abitato in passato e attualmente lo abitano
hanno evoluto molteplici interazioni con la luce solare.Tra queste, la Fotosintesi, e cioè la capacità di catturare e usare l’energia del Sole, rappresenta certamente il processo biologico fondamentale e unico dal quale
dipendono tutti gli organismi vivent
Myocardial blood flow reserve and absolute myocardial blood flow for the assessment of patients with coronary artery disease with or without microvascular dysfunction
No full textThe Arabidopsis Salt Tolerant Related Protein (STRP) is involved Abscisic Acid signaling and biosynthesis
No full textEnvironmental stresses are the main cause of crop loss and reduction in agricultural yield. The alteration of gene expression pattern and the accumulation of stress-related protein are the main strategies activated by plants under unfavourable conditions. Here we characterized the Salt Tolerance Related Protein (STRP) that is a new player in stress response in Arabidopsis thaliana. STRP has been identified in a proteomic screening of temperature stress-responsive proteins of A. thaliana. This protein increases strongly under different abiotic stress conditions and knock out mutants are hypersensitive to cold stress damages. The presence of an ABA responsive element (ABRE) in the STRP promoter led us to investigate the role of STRP in abscisic acid signaling. Abscisic acid (ABA) is a phytohormone rapidly accumulated under different abiotic stress conditions and is also involved in different physiological processes such as seed germination, seed dormancy and, stomata closure. ABA induces the increase of STRP into a total cellular extract of A. thaliana and is able to activate STRP expression. We analysed the effects of the hormone plant growth and development by means strp knock-out mutant. The mutant is hyposensitive to the effects of ABA on seed germination and dormancy, primary root elongation, induction of senescence and stomata closure. strp mutant has an altered expression of the ABA-responsive genes and also the ABA biosynthetic genes are impaired. Our results suggest a role for STRP in ABA signaling and perception
Salt Tolerance-Related Protein (STRP): a new player involved in cold stress response in Arabidopsis thaliana
No full textSalt Tolerance-Related Protein (STRP): a new player involved in cold stress response in Arabidopsis thaliana
No full textThe Salt Tolerance Related Protein (STRP) Mediates Cold Stress Responses and Abscisic Acid Signalling in Arabidopsis thaliana
Full text linkLow temperature stress is one of the major causes of crop yield reduction in agriculture. The alteration of gene expression pattern and the accumulation of stress-related proteins are the main strategies activated by plants under this unfavourable condition. Here we characterize the Arabidopsis thaliana Salt Tolerance Related Protein (STRP). The protein rapidly accumulates under cold treatment, and this effect is not dependent on transcriptional activation of the STRP gene, but on the inhibition of proteasome-mediated degradation. Subcellular localization of STRP was determined by the transient expression of STRP-YFP in A. thaliana protoplasts. STRP is localized into the cytosol, nucleus, and associated to the plasma membrane. Under cold stress, the membrane-associated fraction decreases, while in the cytosol and in the nucleus STRP levels strongly increase. STRP has high similarity with WCI16, a wheat Late Embryogenesis Abundant (LEA)-like protein. Despite no canonical LEA motifs in the STRP sequence are present, physicochemical characterization demonstrated that STRP shares common features with LEA proteins, being a high hydrophilic unstructured protein, highly soluble after boiling and with cryoprotectant activity. To clarify the physiological function of STRP, we characterized the phenotype and the response to low temperature stress of the strp knockout mutant. The mutation causes an equal impairment of plant growth and development both in physiological and cold stress conditions. The strp mutant is more susceptible to oxidative damage respect to the wild type, showing increased lipid peroxidation and altered membrane integrity. Furthermore, the analysis of Abscisic acid (ABA) effects on protein levels demonstrated that the hormone induces the increase of STRP levels, an effect in part ascribable to its ability to activate STRP expression. ABA treatments showed that the strp mutant displays an ABA hyposensitive phenotype in terms of seed germination, root development, stomata closure and in the expression of ABA-responsive genes. In conclusion, our results demonstrate that STRP acts as a multifunctional protein in the response mechanisms to low temperature, suggesting a crucial role for this protein in stress perception and in the translation of extracellular stimuli in an intracellular response
Prognostic Value of 18F-FDG PET/CT Metabolic Parameters in Splenic Marginal Zone Lymphoma
No full textThe present study investigated the potential value of fluorine-18 fluorodeoxyglucose positron emission tomography/computed tomography metabolic features in estimating progression-free survival (PFS) and overall survival (OS) in patients affected by splenic marginal zone lymphoma. The estimated 2-year PFS and OS rates were 78% and 90%, whereas 5-year PFS and OS rates were 63% and 82%. Only lesion to liver maximum standardized uptake value ratio and lesion to blood-pool maximum standardized uptake value ratio were independent prognostic factors for PFS. By contrast, for OS, no positron emission tomography/computed tomography features were correlated with outcome
Spodoptera littoralis oral secretions inhibit the activity of Phaseolus lunatus plasma membrane H+-ATPase
Full text linkBiotic stresses induced by herbivores result in diverse physiological changes in plants. In the interaction between the Lima bean (Phaseolus lunatus) and the herbivore Spodoptera littoralis, the earliest event induced by feeding on leaves is the depolarization of the plasma membrane potential (Vm), which is the results of both mechanical damage and insect oral secretions (OS). Although this herbivore-induced Vm depolarization depends on a calcium-dependent opening of potassium channels, the attacked leaf remains depolarized for an extended period, which cannot be explained by the sole action of potassium channels. Here we show that the plasma membrane H+-ATPase of P. lunatus leaves is strongly inhibited by S. littoralis OS. Inhibition of the H+-ATPase was also found in plasma membranes purified from leaf sections located distally from the application zone of OS, thus suggesting a long-distance transport of a signaling molecule(s). S. littoralis' OS did not influence the amount of the plasma membrane H+-ATPase, whereas the levels of membrane-bound 14-3-3 proteins were significantly decreased in membranes purified from treated leaves. Furthermore, OS strongly reduced the in vitro interaction between P. lunatus H+-ATPase and 14-3-3 proteins. The results of this work demonstrate that inhibition of the plasma membrane H+-ATPase is a key component of the S. littoralis OS mechanism leading to an enduring Vm depolarization in P. lunatus wounded leaves
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