1,720,978 research outputs found
Use of tetraalkyl phenothiazine derivatives as photodynamic agents in the treatment of microbial infections.
No full textPhotodynamic inactivation of microbial pathogens: disinfection of water and prevention of water-borne diseases.
No full textPorphyrins have been shown to act as very efficient photosensitizing agents against a broad number of microbial pathogens, including bacteria, fungi, and protozoa. This property has promising applications at a clinical level for the treatment of infectious diseases by photodynamic therapy. Moreover, this technique is also being used to address environmental problems of high significance, such as the decontamination of wastewaters, the disinfection of fish-farming tanks, the protection of animal species (e.g., amphibians and reptiles) that are endangered by pathogens whose life cycle takes place largely in aqueous media, and the control of populations of noxious insects. Such diversified applications take advantage of the availability of a truly large number of porphyrin derivatives with chemical structures that can be tailored to comply with the physical and chemical properties as well as the biological features of several milieus. In addition, the property typical of porphyrins to absorb essentially all of the wavelengths in the sun emission spectrum allows the promotion of processes largely based on natural resources with significant energy savings and low impact on ecosystems
Porphyrin Photosensitised Processes in the Prevention and Treatment of Water- and Vector-Borne Diseases.
No full textWater- and vector-borne diseases are a global burden which is estimated to cause several million deaths and innumerable cases of sickness every year. These infectious illnesses are emerging or resurging as a result of several factors, such as changes in climate, in public health and demography policy, as well as the spread of resistance to insecticide and drug, and genetic changes in pathogens. Integrated prevention strategies must be developed and implemented in endemic disease areas to reverse the trend of emergent/resurgent water- and vector-borne diseases. With this perspective porphyrins and their analogues, that have been shown to act as very efficient photosensitising agents against a broad number of microbial pathogens (bacteria, fungi, protozoa) and parasitic animals, could represent an important tool for the prevention and control of these pathologies. The application of photosensitised processes can be exploited to address environmental problems of high significance, including the decontamination of waste waters, the disinfection of fish-farming tanks and the control of populations of noxious insects. Such diversified applications take advantage of the availability of a truly large number of porphyrin derivatives with chemical structures which can be tailored to comply with the physical and chemical properties, as well as the biological features of several milieus. In addition, the property typical of porphyrins to absorb essentially all the wavelengths in the sun emission spectrum allows the promotion of processes largely based on natural resources with significant energy saving and low impact on the ecosystems
Gene expression profile in murine 3T3 fibroblasts photosensitised by a tetracationic porphyrin.
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Sunlight-activatable porphyrin photosensitisers as environmentally safe larvicidal agents: studies with larvae of Aedes albopictus
No full textA photodynamic approach for the treatment of spontaneous microbial infections in pet animals.
No full textGene expression profile in murine 3T3 fibroblasts photosensitised by a tetracationic porphyrin
No full textThe interest regarding the study of cell death in the context of photodynamic therapy (PDT) is very deep. Phenomena of apoptosis, autophagia and necrosis in PDT-treated cells have been widely observed, the specific results can depend on the treatment parameters and cell line used.
In order to identify key genes expressed in response to photosensitisation after short term exposure to porphyrins and visible light, a study on the transcriptome of murine 3T3 fibroblast cell cultures was carried out through the microarray approach.
Toward this aim we studied the effect induced by a tetracationic meso-substituted porphyrin ( C12) in this cell line, employing different time incubations and photosensitiser concentrations.
The subcellular localisation of the photosensitiser was investigated on cells which had been incubated with 0.1 μM C12 for 1 h before and after 1 min of irradiation with white light at a fluence rate of 10 mW/cm2. Under these conditions a limited cell mortality occurs (about 5%), hence these samples are suitable for studing initial gene expression.
The analysis of microarray data allowed the identification of 531 differentially expressed genes in treated cells with respect to controls, 67 of which resulted overexpressed. Among these, some genes are involved in cell proliferation and differentiation, such as Wnt11 (7.5 fold change) whose expression in mouse is activated during artery morphogenesis and endoderm development processes. Interestingly, one of the overexpressed genes was found to be the Tumour suppressor candidate 1 (Tusc1), 3-fold more expressed in photosensitised fibroblasts. In order to confirm and validate these data, and to study the time-course accumulation of mRNAs and the dose-dependent effect of porphyrin C12, a detailed characterization of the expression profiles in murine fibroblasts subjected to photosensitisation will be carried out by quantitative real-time PCR (qRT-PCR)
Porphyrin-photosensitized processes: basic studies and applications in medicine and environment.
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