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Direct electric current from low voltage for the inactivation of venom enzymes: may this be a future direction?
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Energetic metabolism of myelinated axons: a correlation among extramitochondrial atp production in myelin and the sheath development.
No full textEnergetic metabolism of myelinated axons: a correlation among extramitochondrial ATP production in myelin and the sheath development.
No full textElectrophoretic separation of purified myelin: a method to improve the protein pattern resolving.
No full textMyelin sheath is a lipid-rich membrane, consisting of 70% lipid and 30% proteins, that is involved in physiological and pathological processes. For this reason its protein composition has been often investigated, principally by two-dimensional electrophoresis; however, the consistent lipid content makes it difficult to obtain good proteins separation. To improve the resolution of myelin proteins in a denaturing monodimensional gel electrophoresis, we examined several mixtures for the denaturation of the sample, utilizing different detergents and reducing agents. The definition of the protein pattern was analyzed by both "Blue Silver" Coomassie staining and Western Blot analysis against myelin basic protein, one of the most represented myelin proteins. The best resolution is observed when the sample was incubated with a mixture containing 1.25% dithiothreitol, 4 M urea, and 1% dodecyl maltoside or 1 % 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate, prior to addition of denaturing agents. In conclusion, this work describes a novel method to improve the separation of myelin proteins in a monodimensional gel electrophoresis. It may be also useful for investigating other lipid-rich samples
A novel model for extremely low frequency electromagnetic field interactions wits menbrane-anchored enzymes
No full textExportability of the mitochondrial oxidative phosphorylation machinery into myelin sheath
No full textWhite matter comprises over half of
the brain, and its role in axonal survival is being
reconsidered, consistently with the observation
that axonal degeneration follows demyelization.
The recent evidence of an extra-mitochondrial
aerobic ATP production in isolated myelin vesicles,
thanks to the expression therein of the mitochondrial
Oxydative Phosphorylation (OXPHOS)
machinery, stands in for myelin playing a
functional bioenergetic role in ATP supply for the
axon. The observation that subunits of the OXPHOS
encoded by the mitochondrial genome are
expressed in myelin, suggests that they can be the
same as those of the inner mitochondrial membrane.
This would mean that the OXPHOS is exportable.
Here the hypothesis is exposed that the
mitochondrion is the unique site of the assembly
of the OXPHOS, so that this is exported to those
sub cellular districts displaying high energy demand,
such as myelin sheath. There the OXPHOS
would display a higher efficiency in oxidative
ATP production than inside the mitochondrion
itself. In this respect, the role of the glia in the
nervous conduction is shed new light and the
oligodendrocyte mitochondrial OXPHOS are
hypothesized to be delivered to nascent myelin
Inactivation of Phospholipase A2 and metalloprotease from Crotalus atrox venom by direct current
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