1,721,118 research outputs found
Identification of a peptide binding to the HIV-1 nucleocapsid protein (NCP7)
No full textIn HIV-1 the nucleocapsid protein (NC) is essential in the virion assembly process. The discovery of substances able to interfere with the HIV-1 NC functions could be a starting point for the design of new drugs that might inhibit the HIV-1 viral infectivity. A single peptide sequence binding to the NC was isolated using a conformationally homogeneous phage displayed-peptide library. The minimal requirement for the NCp7/peptide interaction was the NCp7 region encompassing the second zinc finger
A hypoxic signature marks tumors formed by disseminated tumor cells in the BALB-neuT mammary cancer model
Full text linkMetastasis is the final stage of cancer progression. Some evidence indicates that tumor cell dissemination occurs early in the natural history of cancer progression. Disseminated tumor cells (DTC) have been described in the bone marrow (BM) of cancer patients as well as in experimental models, where they correlate with later development of metastasis. However, little is known about the tumorigenic features of DTC obtained at different time points along tumor progression. Here, we found that early DTC isolated from BM of 15-17 week-old Her2/neu transgenic (BALB-neuT) mice were not tumorigenic in immunodeficient mice. In contrast, DTC-derived tumors were easily detectable when late DTC obtained from 19-22 week-old BALB-neuT mice were injected. Angiogenesis, which contributes to regulate tumor dormancy, appeared dispensable to reactivate late DTC, although it accelerated growth of secondary DTC tumors. Compared with parental mammary tumors, gene expression profiling disclosed a distinctive transcriptional signature of late DTC tumors which was enriched for hypoxia-related transcripts and was maintained in ex-vivo cell culture. Altogether, these findings highlight a different tumorigenic potential of early and late DTC in the BALB-neuT model and describe a HIF-1 alpha-related transcriptional signature in DTC tumors, which may render DTC angiogenesis-competent, when placed in a favourable environment
Mechanism of translational initiation in prokaryotes. Evidence for a direct effect of IF2 on the activity of the 30 S ribosomal subunit.
No full textCritical roles for Rictor/Sin1 complexes in IFN-dependent gene transcription and generation of antiproliferative responses.
No full textReinitiation of protein synthesis in Escherichia coli can be induced by mRNA cis-elements unrelated to canonical translation initiation signals.
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Characterization of the str operon genes from Spirulina platensis and their evolutionary relationship to those of other prokaryotes.
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