1,720,971 research outputs found
Steroid hormone receptors in normal, dysplastic and neoplastic feline mammary tissues and their prognostic significance
No full textThe expression of oestrogen-a and progesterone receptors was determined in 13 normal, 21 dysplastic and 53 neoplastic feline mammary tissues. Expression of the receptors was correlated with cell proliferation, as assessed by the MIB-1 immunolabelling index, and with the clinical course of the disease. The expression of oestrogen receptors was significantly higher in healthy tissues and in adenosis than in neoplastic lesions, and the levels of progesterone receptors increased in fibroadenomatous changes and in 'in situ' carcinomas but decreased in invasive carcinomas. The oestrogen and progesterone receptor status of the invasive carcinomas did not correlate either with the histological parameters or with the overall survival of the cats, although the oestrogen receptor-negative tumours had a poor prognosis. Oestrogen receptor-positive neoplasms had a significantly lower MIB-1 immunolabelling index than oestrogen receptor-negative neoplasms
Valutazione comparativa di alcune metodiche per la determinazione delle IgG nel siero del puledro e nel colostro della fattrice
No full textThe role of VEGF and its receptor flk-1 (KDR) in the regulation of angiogenesis in feline mammary tumours: an inventory in search of autocrine and paracrine loops
No full textEspressione immunoistochimica di Vascular Endothelial Growth Factor (VEGF) e del suo recettore flk-1 (KDR) nelle neoplasie mammarie della gatta: studio prelieminare sul possibile meccanismo d’azione di tipo autocrino o paracrino
No full textEspressione immunoistochimica dei recettori per estrogeno e progesterone in tessuti mammari felini sani, displastici e neoplastici e significato prognostico nelle forme maligne
No full textSteroid receptor expression and cell proliferation in normal, dysplastic, and neoplastic feline mammary tissues and their prognostic significance in invasive carcinomas
No full textDensitometric analysis of Western blot assays for feline immunodeficiency virus antibodies
No full textWestern blot (WB) strips for antibodies directed to feline immunodeficiency virus (FIV) were analysed using reflectance densitometry by a semiautomatic densitometer. This method was used to quantify the antibody responses to different FIV proteins in both vaccinated and naturally or experimentally-infected cats. In order to increase reproducibility, reagents and protocols were accurately standardised and internal controls were added. In a first format, an internal control band consisting of feline IgG was added to each blot to minimise the effect of band intensity variation. In a second format, antibody concentrations were calculated from the ratio of the dens ities produced by test sera and by positive and negative standard sera. The sera under scrutiny were also examined by standard enzyme-linked immunosorbent assay (ELISA) and the results obtained compared with those of the corresponding WB. A statistically significant positive correlation was found between the results obtained with the two methods, and this was especially evident when ELISA titres were compared to corrected WB values (P = 0.001). Densitometric analysis of WB assays allowed to quantify the antibodies against FIV proteins and might be useful to investigate possible humoral immune correlates of protection in FIV vaccination studies and antibody production in the early phase of infection. The quantitation of antibodies to Gag and Env FIV antigens might be used to obtain further informations on the course of FIV disease, as previously demonstrated in human immunodeficiency virus-1 (HIV-1) infections. (C) 2001 Elsevier Science B.V. All rights reserved
Espressione immunoistochimica della cicloossigenasi-2 (COX-2) in tessuti mammari sani, iperplastici e neoplastici di gatta: correlazione con parametri clinico-patologici e prognosi
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