38,455 research outputs found
Atrial natriuretic factor and C-type natriuretic peptide induce retraction of human thyrocytes in monolayer culture via guanylyl cyclase receptors
No full textThe natriuretic peptides signal through three receptor subtypes, of which two (NPR-A and NPR-B) are membrane-bound guanylyl cyclases for which the principal ligands are respectively atrial natriuretic factor (ANF) and C-type natriuretic peptide (CNP). In the human thyroid cell, a third receptor, NPR-C, has been implicated in the regulation of thyroglobulin, but functional roles for NPR-A and NPR-B have not yet been defined. In the present study we used RT-PCR to identify transcripts of all three receptor subtypes, both in human thyroid and in HTU-5 cells, a long-term culture of thyroid-derived cells. Both ANF and CNP induced a twofold increase in intracellular cGMP content in HTU-5 cells. Morphologic changes (a significant increase in cells of the retracted phenotype) were observed in ANF- and CNP-treated cells within 3 and 5 h of treatment respectively. Significant increases in retracted cell number were induced by ANF and CNP, but not the NPR-C-specific ring-deleted ANF analog, C-ANF(4-23), during a 15-day treatment. All three natriuretic peptides, however, induced a small (15-20%) but significant (P<0.001) increase in DNA content per well. The stable analog of cGMP, 8-bromo-cGMP (8-BrcGMP; 1 mM), also increased the number of retracted HTU-5 cells, and was equipotent with the cAMP analog, 8-BrcAMP, in this effect. The cGMP-dependent protein kinase inhibitor, KT5823, however, had no significant effect on the ANF-induced increase in numbers of retracted cells. These results suggest that the actions of NPR-A and NPR-B, functional receptors in the human thyroid cell, may in part be mediated by cGMP-induced alterations in the cytoskeleton
Elastic-DF: Scaling Performance of DNN Inference in FPGA Clouds through Automatic Partitioning
No full textCustomized compute acceleration in the datacenter is key to the wider roll-out of applications based on deep neural network (DNN) inference. In this article, we investigate how to maximize the performance and scalability of field-programmable gate array (FPGA)-based pipeline dataflow DNN inference accelerators (DFAs) automatically on computing infrastructures consisting of multi-die, network-connected FPGAs. We present Elastic-DF, a novel resource partitioning tool and associated FPGA runtime infrastructure that integrates with the DNN compiler FINN. Elastic-DF allocates FPGA resources to DNN layers and layers to individual FPGA dies to maximize the total performance of the multi-FPGA system. In the resulting Elastic-DF mapping, the accelerator may be instantiated multiple times, and each instance may be segmented across multiple FPGAs transparently, whereby the segments communicate peer-to-peer through 100 Gbps Ethernet FPGA infrastructure, without host involvement. When applied to ResNet-50, Elastic-DF provides a 44% latency decrease on Alveo U280. For MobileNetV1 on Alveo U200 and U280, Elastic-DF enables a 78% throughput increase, eliminating the performance difference between these cards and the larger Alveo U250. Elastic-DF also increases operating frequency in all our experiments, on average by over 20%. Elastic-DF therefore increases performance portability between different sizes of FPGA and increases the critical throughput per cost metric of datacenter inference. Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Computer Engineerin
Desempenho agronômico de cultivares de batata-doce em sistema orgânico no Assentamento Rural Chapadinha, Brasília-DF.
No full textO objetivo deste trabalho foi avaliar a produtividade de cultivares de batata-doce em sistema orgânico no assentamento Chapadinha, Brasília-DF
Radiolabelling and preclinical characterisation of [89Zr]Zr-Df-ATG-101 bispecific to PD-L1/4-1BB
No full textPurpose: ATG-101, a bispecific antibody that simultaneously targets the immune checkpoint PD-L1 and the costimulatory receptor 4-1BB, activates exhausted T cells upon PD-L1 crosslinking. Previous studies demonstrated promising anti-tumour efficacy of ATG-101 in preclinical models. Here, we labelled ATG-101 with 89Zr to confirm its tumour targeting effect and tissue biodistribution in a preclinical model. We also evaluated the use of immuno-PET to study tumour uptake of ATG-101 in vivo. Methods: ATG-101, anti-PD-L1, and an isotype control were conjugated with p-SCN-Deferoxamine (Df). The Df-conjugated antibodies were radiolabelled with 89Zr, and their radiochemical purity, immunoreactivity, and serum stability were assessed. We conducted PET/MRI and biodistribution studies on [89Zr]Zr-Df-ATG-101 in BALB/c nude mice bearing PD-L1-expressing MDA-MB-231 breast cancer xenografts for up to 10 days after intravenous administration of [89Zr]Zr-labelled antibodies. The specificity of [89Zr]Zr-Df-ATG-101 was evaluated through a competition study with unlabelled ATG-101 and anti-PD-L1 antibodies. Results: The Df-conjugation and [89Zr]Zr -radiolabelling did not affect the target binding of ATG-101. Biodistribution and imaging studies demonstrated biological similarity of [89Zr]Zr-Df-ATG-101 and [89Zr]Zr-Df-anti-PD-L1. Tumour uptake of [89Zr]Zr-Df-ATG-101 was clearly visualised using small-animal PET imaging up to 7 days post-injection. Competition studies confirmed the specificity of PD-L1 targeting in vivo. Conclusion: [89Zr]Zr-Df-ATG-101 in vivo distribution is dependent on PD-L1 expression in the MDA-MB-231 xenograft model. Immuno-PET with [89Zr]Zr-Df-ATG-101 provides real-time information about ATG-101 distribution and tumour uptake in vivo. Our data support the use of [89Zr]Zr-Df-ATG-101 to assess tumour and tissue uptake of ATG-101
Hexágono '71 - df
No full textTrabajos plásticos:
Edgardo Vigo.
Eduardo Leonetti.
Juan C. Romero.
Jorge Glusberg.
Horacio Zabala.
Amelia Toledo.
Endre Tôt.
Michael Joseph Phillips.
Guillermo Deisler.
Clemente Padín.
Windsor Mckey.
Textos:
Homage to Boredom contest number two, Chuck Stake Enterprizes.Centro de Arte Experimental Vig
(DF)-Spaces of type and
No full textSome locally convex properties of the spaces of the bounded continuous functions on a completely regular Hausdorff space X with values in a (DF-space) E are studied and applied to the (DF)-spaces of type (e.g., see [S]).The following are our main results:
1. is a (DF)-space if and only if E is a (DF)-space.
2.For a (DF)-space E, is quasi barrelled if and only if either (i)X is pseudocompact and E is quasibarrelled or (ii) X is not pseudocompact and the bounded subsets of E are metrizaable.
3. If and if each is dominated by some , then (resp., ) is a (DF)-space if and only if E is a (DF)-space.
4. Let X be a locally compact and σ-compact space, and E a (DF)-space. Then is quasibarrelled if and only if (i) E is quasibarrelled and satisfies condition or (ii) the bounded subsets of E are metrizable and satisfies condition (D)
The average DF incidence rates and increment of average DF incidence rates.
No full textA, B, &C were generated by ArcGIS 10.0 (Environmental Systems Research Institute, RedLands, California, U.S.A.). The spatial distributions for annual mean DF incidence rates during 2006±2011; (B) The spatial distributions for annual mean DF incidence rates during 2012±2014; (C) The spatial distribution for increment of annual mean DF incidence rates from 2006±2011 to 2012±2014.</p
C-075 EMBARAZO, UNO DE LOS PRINCIPALES MOTIVOS DE DISCRIMINACIÓN EN EL DF: COPRED
No full textC-075 EMBARAZO, UNO DE LOS PRINCIPALES MOTIVOS DE DISCRIMINACIÓN EN EL DF: COPREDC-075 EMBARAZO, UNO DE LOS PRINCIPALES MOTIVOS DE DISCRIMINACIÓN EN EL DF: COPREDjp
C-075 EMBARAZO, UNO DE LOS PRINCIPALES MOTIVOS DE DISCRIMINACIÓN EN EL DF: COPRED
No full textC-075 EMBARAZO, UNO DE LOS PRINCIPALES MOTIVOS DE DISCRIMINACIÓN EN EL DF: COPREDC-075 EMBARAZO, UNO DE LOS PRINCIPALES MOTIVOS DE DISCRIMINACIÓN EN EL DF: COPREDDO
Total IgE, total IgG2a, Df-specific IgE, and Df-specific IgG2a levels in serum.
No full text<p>Serum total IgE (A) and total IgG2a (C) were measured by ELISA and are shown as serum concentrations. Df-specific IgE (B) and Df-specific IgG2a (D) were measured based on optical density. Results are expressed as mean ±SD of 6 mice per group. *p<0.05 vs. CJ-1+Df/Df group.</p
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